Bioactive Compounds From Purple Roselle Calyx (Hibiscus Sabdariffa L.) Extract Using Multistage Countercurrent Method

Triyastuti, Meilya Suzan; Anwar, Nadiem

doi:10.20473/mgi.v17i1.1-10

Cited by 2 publications

(2 citation statements)

References 42 publications

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“…The production of the enriched extract using the two-stage countercurrent method can be attributed to the higher concentration gradient that is established during the dynamic circulation of fresh material and water. This phenomenon is in agreement with the findings from Triyastuti and Anwar [83] in the three-stage countercurrent extraction at 70 • C of anthocyanins (2815.43 mg/L) and quercetin (59.25 mg/L) from purple roselle calyxes.…”

Section: Quantification Of Phenolics In Cabernet Sauvignon Grape Poma...supporting

confidence: 92%

Revitalizing Unfermented Cabernet Sauvignon Pomace Using an Eco-Friendly, Two-Stage Countercurrent Process: Role of pH on the Extractability of Bioactive Phenolics

et al. 2022

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As the major byproduct of the winemaking industry, grape pomace remains an untapped source of valuable bioactive phenolic compounds. This study elucidated the optimal aqueous extraction parameters for maximizing phenolic extractability, while avoiding the use of harsh conventional solvents and limiting water usage, from Cabernet Sauvignon grape pomace in which the red grape was processed for white wine. In the single-stage aqueous extraction process (AEP), the concurrent impact of pH (2.64–9.36), solids-to-liquid ratio (SLR, g pomace/mL water) (1:50–1:5), and temperature (41.6–58.4 °C) on the total phenolic content (TPC) of Cabernet Sauvignon pomace was evaluated alongside a kinetic study (15–90 min). Optimal single-stage extraction conditions (pH 9.36, 1:50 SLR, 50 °C, 75 min) guided the development of a two-stage countercurrent extraction process (pH 9.36, 1:10 SLR, 50 °C, 75 min) to further reduce water consumption without compromising overall extractability. The countercurrent process reduced fresh water usage by 80%, increased the TPC of the extracts by 18%, and improved the in vitro antioxidant activities (ABTS and ORAC) of the extracts. Untargeted metabolomics enabled the identification of a diverse pool of phenolics, especially flavonol glycosides, associated with grape pomace, while further phenolic quantitation detected improvements in the release of commonly bound phenolics such as ferulic acid, p-coumaric acid, syringic acid, and protocatechuic acid in alkaline extracts compared to the ethanolic extract. This investigation provides an efficient, eco-friendly extraction strategy suitable for applications in functional food, beverage, nutraceutical, and cosmetic industries.

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Section: Quantification Of Phenolics In Cabernet Sauvignon Grape Poma...supporting

confidence: 92%

Revitalizing Unfermented Cabernet Sauvignon Pomace Using an Eco-Friendly, Two-Stage Countercurrent Process: Role of pH on the Extractability of Bioactive Phenolics

et al. 2022

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“…As it is known that roselle plant flower rich in organic acids, phenolic compounds, flavonoids and anthocyanins and antioxidant [25].Amongst these, flavonoids, known as polyphenolic compounds, show antibacterial property aginst a wide range of pathogenic microorganisms, including multidrug resistant bacteria due to their tendency to retard the microbial growth [26].…”

Section: Total Plate Countmentioning

confidence: 99%

Effect of flower extract of Roselle (Hibiscus sabdariffa L) on microbial quality of raw milk of Etawa goats

Huda¹,

Nuraini²,

Setyaningrum³

et al. 2023

GSC Biol. Pharm. Sci.

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Etawa goat milk is the name for dairy products from a hybrid goat named Etawa. In Indonesia, goat milk is usually marketed and consumed fresh, without proper sterilization in the production process. As a raw product, Etawa goat milk has the potential to be contaminated with various microbes. In order to improve the microbial quality of Etawa's raw milk, we conducted a study aimed to evaluate the effect of adding aqueous extract of Roselle flower Hibiscus sabdarrifa L on the milk content of bacteria. Five groups of fresh milk samples, each 25 ml, were given roselle flower extract sequentially of 0.5 ml, 1.0 ml, 1.5 ml, 2.0 ml and 2.5 ml with a repetition of 5 times. Before adding the extract, the type of bacteria identified was Escherichia coli, Lactobacillus sp, and Staphylococcus sp with a total bacterial content of 1,569,586 CFU/ml. After adding extracts of 0.5 ml, 1 ml, 1.5 ml, 2 ml, and 2.2 ml, the total number of bacteria decreased to 1,078,660 CFU/ml, 1,010,423 CFU/ml, 938,602 CFU/ml, 882,.873 CFU/ml, and 874,238 CFU/ml respectively. Thus it can be concluded that roselle flower extract has the potential to be used as an ingredient to improve the microbial quality of fresh milk of Etawa goats.

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Bioactive Compounds From Purple Roselle Calyx (Hibiscus Sabdariffa L.) Extract Using Multistage Countercurrent Method

Cited by 2 publications

References 42 publications

Revitalizing Unfermented Cabernet Sauvignon Pomace Using an Eco-Friendly, Two-Stage Countercurrent Process: Role of pH on the Extractability of Bioactive Phenolics

Revitalizing Unfermented Cabernet Sauvignon Pomace Using an Eco-Friendly, Two-Stage Countercurrent Process: Role of pH on the Extractability of Bioactive Phenolics

Effect of flower extract of Roselle (Hibiscus sabdariffa L) on microbial quality of raw milk of Etawa goats

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