Bioactive compounds from Hypericum humifusum and Hypericum perfoliatum: inhibition potential of polyphenols with acetylcholinesterase and key enzymes linked to type-2 diabetes

Abstract

Context: Natural products are reported to have a wide spectrum of pharmacological properties such as antimicrobial, anti-inflammatory and anti-cholinesterase. The genus Hypericum (Hypericaceae) is a source of a variety of molecules with different biological activities, notably hypericin and various phenolics.Objectives: The goals of the present work were the determination of total phenolic and flavonoid content, hypericin and hyperforin concentration as well as the evaluation of biological of Hypericum humifusum L. (Hhu) and Hypericum perfoliatum L. (Hper).Materials and methods: The various extracts of aerial parts were powdered, and then extracted with methanol. Antibacterial activity was performed according to minimum inhibitory concentration (MIC) and minimum bactericidal (MBC) methods against four Gram-positive bacteria, four Gram-negative bacteria and yeast.Results: The results revealed that H. humifusum, bear the highest total phenolic and flavonoid content (48–113 mg GAE/g and 8–41 mg RE/g, respectively) as well as hypericin (60–90 mg/g) and hyperforin (8–30 mg/g) concentration. Both species showed significant antioxidant activity as revealed by DPPH, FRAP, ABTS, and metal chelating assays. H. humifusum exhibited a strong acetylcholinesterase (3.86–4.57 mg GALAEs/g), α-glucosidase (0.73–2.55 mmol ACEs/g) and α-amylase (3–8 mmol ACEs/g) inhibitory activity. The extract of H. humifusum exhibited strong antibacterial activity mainly against Staphylococcus epidermidis, Staphylococus aureus, and Enterococcus faecium (MIC values ranging from 200 to 250 μg/mL). The highest antifungal activity was showed for H. perfoliatum extract (MIC value = 250 μg/mL).Conclusion: The data suggest that H. humifusum could be used as valuable new natural agents with functional properties for pharmacology industries.

Chemical Composition, Antioxidant Potential and Enzymes Inhibitory Properties of Globe Artichoke By‐Products

“…In previous works, authors have found positive correlation between flavonoid amounts and enzyme inhibitory effects . On the other hand, there was no correlation between antioxidant activity and enzyme inhibitory effect of extracts.…”

Screening of Peruvian Medicinal Plants for Tyrosinase Inhibitory Properties: Identification of Tyrosinase Inhibitors in Hypericum laricifolium Juss

“…Its known that genus Hypericum has a variety of molecules with different biological activities, among them the content of hypericin and various phenolics is considerable, exhibiting wide pharmacological activities such as anti-inflammatory, anti-cholinesterase, antimicrobial, antiviral and antioxidant properties [ 40 ]. The species Hypericum humifusum and Hypericum perfoliatum have great content of hypericin and phloglucinols.…”

Chemical Composition and Biological Activities of the Essential Oils of Leptospermum petersonii and Eucalyptus gunnii

“…E. gunnii EO showed good activity against E. coli, although it was weaker than that exerted on other E. coli strains (Bugarin et al, 2014). It is well known that the resistance/sensitivity to an antimicrobial agent may vary among different strains of the same species (Del Monte et al, 2015). However, we should stress that the inhibition zone induced by E. gunnii EO was almost double than tetracycline (Figure 4A).…”