Bioactive compound isolated from marine Bacillus safensis MB8 and their cytotoxicity potential

Abstract

Background: Marine bacteria represent a potential source for the production of medically useful compounds against cancer, diabetes, inflammation, infection, etc. In the present study, isolation, characterization and identification of Objectives: bioactive compound from marine MB8. Breast cancer (MCF-7) cell lines to determine the Bacillus safensis Methods: cytotoxicity activity of bioactive compound produced from marine MB8. Bacillus safensis Results: Marine Bacillus safensis MB8 (Genbank accession no. KJ531643) isolated from deep sea sediments of the Bay of Bengal, India, produced a bioactive compound, Bis (2-ethylhexyl) benzene-1,2-dicarboxylate (BEHBD) with a molecular formula of C H O. The molecular 24 38 4 ion m/z 391 (M). The MTT assay based cytotoxicity assessment of BEHBD in MCF-7 cancer cells revealed the IC peak at + 50 to be 49.8µg/ml. The acridine-orange and ethidium bromide (AO/EB) staining of the BEHBD treated cancer cells showed typical characteristic of apoptosis such as nuclear condensation, cell shrinkage and formation of apoptotic bodies. Conclusion: Potent cytotoxicity compound of Bis (2-ethylhexyl) benzene-1, 2-dicarboxylate (BEHBD) produced from marine MB8. It was undoubtedly confirmed to BEHBD is a proved cytotoxicity compound. This is the first Bacillus safensis reported to BEHBD produced by marine MB8.