Bioactive components and mechanisms of Chinese poplar propolis alleviates oxidized low-density lipoprotein-induced endothelial cells injury

Chang, Huasong; Yuan, Wenwen; Wu, Haizhu; Yin, Xusheng; Han, Xuan

doi:10.1186/s12906-018-2215-8

Cited by 24 publications

(16 citation statements)

References 35 publications

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“…Our results clearly show that M. trifoliata extracts from plants derived from in vitro cultures play an important role in DNA protection of the tested cells. This is consistent with other literature data showing that polyphenol-rich natural products also inhibit the increases in cleaved PARP level [61, 62]. Similarly, the level of phosphorylated H2A.X, another determinant of DNA damage, has previously been found to be reduced under the influence of the black tea or Camptosorus sibiricus extract [63] in other cells.…”

Section: Discussionsupporting

confidence: 92%

An Evaluation of the DNA-Protective Effects of Extracts fromMenyanthes trifoliataL. Plants Derived fromIn VitroCulture Associated with Redox Balance and Other Biological Activities

Kowalczyk

Sitarek

Skała

et al. 2019

Oxidative Medicine and Cellular Longevity

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Menyanthes trifoliata L. is a valuable medical plant found in Europe, North America, and Asia, which grows on peat bogs and swamps. It has long been used in folk medicine as a remedy for various ailments. This is the first report to demonstrate the protective antioxidant and anti-inflammatory properties of aqueous methanolic extracts derived from the aerial parts (MtAPV) and roots (MtRV) of in vitro grown plants on human umbilical vein endothelial cells (HUVECs). It describes the influence of the tested extracts on the expression of antioxidant (HO-1, NQO1, NRF2, kEAP1, and GCLC) and inflammation-related genes (IL-1α, IL-1β, IL-6, TNF-α, and IFN-γ) in cells stimulated with H2O2 or LPS, respectively. In addition, M. trifoliata extracts were found to moderately affect the growth of certain bacterial and fungal pathogens, with the strongest antibacterial effect found against Pseudomonas aeruginosa and Enterococcus faecalis. M. trifoliata extracts demonstrated protective effects against mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) damage caused by ROS, decreasing the numbers of mtDNA lesions in the ND1 and ND2 genes and nDNA damage in the TP53 and HPRT1 genes and reducing cleavage in PARP1- and γ-H2A.X-positive cells. The root extract of in vitro M. trifoliata (MtRV) appears to have better anti-inflammatory, antioxidant, antimicrobial, and protective properties than the extract from the aerial part (MtAPV). These differences in biological properties may result from the higher content of selected phenolic compounds and betulinic acid in the MtRV than in the MtAPV extract.

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Section: Discussionsupporting

confidence: 92%

An Evaluation of the DNA-Protective Effects of Extracts fromMenyanthes trifoliataL. Plants Derived fromIn VitroCulture Associated with Redox Balance and Other Biological Activities

Kowalczyk

Sitarek

Skała

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…The extract was able to protect cells from ox‐LDL‐induced cell death after 48 hr (Figure 3c), which was not observed after 72 hr (Figure 3d) possibly due to the instability of the phenolic antioxidant compounds in the extract, suggesting an optimal time of action in these conditions and a cardiovascular protective effect. Polyphenols such as resveratrol (Zhang et al, ), vitexin (Zhang et al, ), and others like those present in propolis processed by bees (Chang, Yuan, Wu, Yin, & Xuan, ), attenuated the cell injury caused by ox‐LDL‐mediated oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro

Silva

Morelli

Pavan

et al. 2020

Phytotherapy Research

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Beetroot is an herb used worldwide as a food product, raw material for food industry, ethanol production and source of food coloring. Beet leaves are an unconventional food with antioxidant properties, which might neutralize reactive oxygen species (ROS) induced by oxidized Low‐Density Lipoprotein (LDL) present in dyslipidemias. This study aimed to elucidate the effects of beet leaves on the suppression of LDL oxidative processes. Beet leaves extract was produced, characterized, and tested for its antioxidant capacity using endothelial cells in vitro. A model of human umbilical vein endothelial cells was used in various tests, including viability assay, molecular analysis of antioxidant genes, ROS labeling, and macrophage adhesion assay. The extract improved the antioxidative protection of endothelial cells against different agents including oxidized LDL‐cholesterol and H2O2. It acted on ROS directly due to its high content of natural antioxidants, but also due to the activation and improvement of cellular defenses such as Superoxide dismutase 1, Superoxide dismutase 2, and catalase. The inhibition of LDL‐mediated oxidative effects on endothelial cells may turn this unconventional food a functional food with great potential for phytotherapy of atherosclerosis as an adjuvant for medicinal treatments.

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“…The SRB assay was used to determine cell viability as described in the literature . Briefly, cells were precipitated with 100 μL 10% trichloroacetic acid for 1 hour at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of ulinastatin on hepatic ischemia reperfusion injury through autophagy activation in Chang liver cells

Zhao

Cai

Zhou

et al. 2019

J of Cellular Biochemistry

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This study aimed to investigate the protective effect of ulinastatin in hepatic ischemia‐reperfusion progress, involving its association with the role of autophagy during hypoxia‐induced hypoxia‐reoxygenation injury in vitro. The model of hepatic hypoxia/reoxygenation (H/R) injury in Chang liver cells was established. After treatment with ulinastatin at the doses of 10, 100, and 1000 U/mL in H/R liver cells, the cell proliferation was significantly increased, morphological damage was reduced, and the cell apoptosis rate was decreased. The protein levels of antiapoptotic myeloid cell leukemia‐1 (Mcl‐1) and caspase‐3 were upregulated, and C‐PARP protein was downregulated. Meanwhile, ulinastatin led to an increase in the messenger RNA and protein levels of autophagy maker Unc‐like kinase 1 (ULK1), Beclin‐1, and microtubule‐associated protein 1 light chain 3 (LC‐3) and a decrease in p62. Then, 3‐methyladenine (3‐MA), an inhibitor of autophagy, made morphological damage and cell apoptosis worsen in ulinastatin‐treated H/R liver cells. And the expression levels of caspase‐3, C‐PARP, p62, Beclin‐1, and LC‐3, proteins were also reversed by 3‐MA. Taken together, our results demonstrate that ulinastatin inhibited the hepatic H/R injury in Chang liver cells, which was, to some extent, related to the autophagy activation.

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Bioactive components and mechanisms of Chinese poplar propolis alleviates oxidized low-density lipoprotein-induced endothelial cells injury

Cited by 24 publications

References 35 publications

An Evaluation of the DNA-Protective Effects of Extracts fromMenyanthes trifoliataL. Plants Derived fromIn VitroCulture Associated with Redox Balance and Other Biological Activities

An Evaluation of the DNA-Protective Effects of Extracts fromMenyanthes trifoliataL. Plants Derived fromIn VitroCulture Associated with Redox Balance and Other Biological Activities

Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro

Protective effect of ulinastatin on hepatic ischemia reperfusion injury through autophagy activation in Chang liver cells

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