Bioactive coatings on Portland cement substrates: Surface precipitation of apatite-like crystals

Gallego, Daniel; Higuita, Natalia; García, Felipe Legorreta; Ferrell, Nicholas; Hansford, Derek J.

doi:10.1016/j.msec.2007.04.020

Cited by 33 publications

(26 citation statements)

References 25 publications

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“…In previous research, we were able to induce surface precipitation of apatite-like crystals on carbonated cement substrates via exposure to a concentrated CaP solution to potentially increase or impart a bioactive response. 32 The results of the present study suggest that carbonated Portland cement could exhibit bioactive behavior in physiologic solutions by itself. However, pre-precipitation of apatite-like phases could help to induce a stronger bioactive response in this material.…”

Section: Discussionmentioning

confidence: 51%

Portland cement for bone tissue engineering: Effects of processing and metakaolin blends

Gallego‐Perez

Higuita‐Castro

Quiroz³

et al. 2011

J Biomed Mater Res

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The need for a suitable scaffolding material for load bearing bone tissue engineering still has yet to be met satisfactorily. In this study, Portland cement and Portland cement/metakaolin (MK) blends were processed to render them biologically and mechanically suitable for such application. Portland cement was mixed with MK at different ratios. The slurries were hydrated under atmospheric (noncarbonated samples) and high-CO₂ conditions (carbonated samples). The mechanical properties were characterized via compressive tests. The bioactivity was analyzed in a simulated body fluid solution. Scanning electron microscopy and energy dispersive spectroscopy were used to evaluate sample morphology and chemistry. The cytocompatibility (direct contact assay, MTT test, and alkaline phosphatase activity) was tested using human osteoblast-like cells. Cell responses were observed via conventional and electron microscopy. The results showed that the implementation of MK did not significantly influence the mechanical properties. All the samples evidenced bioactive behavior. Cell experiments confirmed a highly cytotoxic response to the noncarbonated specimens. The introduction of MK as well as the CO₂ pretreatment significantly improved the cytocompatibility of the specimens. These results show that properly processed Portland cement and Portland cement/MK blends could present suitable properties for the development of load-bearing scaffolding structures in bone tissue-engineering applications.

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Section: Discussionmentioning

confidence: 51%

Portland cement for bone tissue engineering: Effects of processing and metakaolin blends

Gallego‐Perez

Higuita‐Castro

Quiroz³

et al. 2011

J Biomed Mater Res

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“…Early CaP precipitation on non-carbonated samples could probably be attributed to the higher pH of these specimens [4,13]. In previous research, we were able to induce surface precipitation of apatite-like crystals on carbonated cement substrates via exposure to a concentrated calcium phosphate solution to potentially enhance the bioactivity of the material [16]. The results presented in this work showed, however, that carbonated Portland cement is capable of inducing CaP precipitation when exposed to physiologic-like solutions (indicator of bioactivity).…”

Section: Resultsmentioning

confidence: 92%

Biocompatibility, Bioactivity and Mechanical Properties of Portland Cement and Portland Cement-Metakaolin Blends for Bone Tissue Engineering Applications

Gallego

Higuita

García³

et al. 2008

MRS Proc.

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We studied the potential applications of Portland cement and Portland cementMetakaolin blends as scaffolding materials for load bearing bone tissue engineering. Cementitious pastes were prepared by mixing Portland cement and Metakaolin at different ratios (100:0, 85:15), and hydrated under a concentrated CO 2 atmosphere (carbonated pastes). Pastes fabricated similarly, but hydrated under normal atmospheric conditions were used for comparison (non-carbonated pastes). Compressive tests were run to evaluate the mechanical properties of the pastes. The bioactivity of the samples was tested in a simulated body fluid (SBF) solution for 1 and 4 days. Sample morphology and chemistry were characterized via scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), respectively. The cytocompatibility was studied using human osteosarcoma (HOS) cell cultures and the direct contact assay. Mechanical characterization did not show significant differences in the compressive strength of the blends compared to pure cement controls. The bioactivity test revealed that the pastes induced surface precipitation of calcium phosphate (CaP) when exposed to the SBF solution (as confirmed by SEM and EDS). Non-carbonated pastes induced early CaP precipitation. Cytocompatibility experiments showed that the carbonated blends allowed adequate cell growth. Non-carbonated blends presented a highly cytotoxic behavior. The introduction of Metakaolin did not affect the cytocompatibility of the samples. These results show that Portland cement and Portland cement-Metakaolin blends could present suitable characteristics for applications as scaffolding materials in load bearing bone tissue engineering.

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“…However, as a material containing a large amount of calcium hydroxide, which is known to produce a pH of around 12.4 when in contact with water, the hydrated cement paste may induce cytotoxicity 45 . Such a result has been found in previous studies on biocompatibility and cytotoxicity of calcium hydroxide based root canal sealers 46,47 .…”

Section: Discussionmentioning

confidence: 99%

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Torkittikul¹,

Chaipanich²

2009

ScienceAsia

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Biomaterials containing calcium silicate have been widely used in dental and bone repaired applications. This study investigated mechanical and in vitro biological properties of ordinary and white Portland cement both of which are composed mainly of calcium silicates. The time of setting was determined using a Vicat needle. Compressive strength was measured at the age of 1, 3, and 7 days. The 1 day set Portland cement pastes were soaked in a simulated body fluid to investigate the ability of the material to form a bond with living bone tissue. X-ray diffraction traces of samples following immersion in simulated body fluid demonstrated that both cement pastes form a hydroxyapatite layer within 7 days but white Portland cement shows a noticeably higher amount of apatite crystal than that of ordinary Portland cement. Scanning electron micrographs reveal a clear presence of hydroxyapatite on the surface of white, but not in ordinary Portland cement. In addition, white Portland cement can achieve a compressive strength of 30 MPa within 24 h after curing in distilled water at human temperature.

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Bioactive coatings on Portland cement substrates: Surface precipitation of apatite-like crystals

Cited by 33 publications

References 25 publications

Portland cement for bone tissue engineering: Effects of processing and metakaolin blends

Portland cement for bone tissue engineering: Effects of processing and metakaolin blends

Biocompatibility, Bioactivity and Mechanical Properties of Portland Cement and Portland Cement-Metakaolin Blends for Bone Tissue Engineering Applications

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