Bioactive and ACE binding properties of three synthetic peptides assessed by various spectroscopy techniques

Tanzadehpanah, Hamid; Asoodeh, Ahmad; Mahaki, Hanieh; Mostajabodave, Zeinab; Chamani, Jamshidkhan; Mojallal-Tabatabaei, Zahra; Emtenani, Shirin; Emtenani, Shamsi; Moradi, Mohammadreza

doi:10.1016/j.procbio.2016.09.017

Cited by 26 publications

(9 citation statements)

References 34 publications

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“…Several forces play a considerable role in forming the NP–protein complex. These forces include hydrophilic and hydrophobic interactions . Therefore, to explore the exact nature of binding force present in the SiO 2 NPs–Hb interaction, thermodynamic parameters were determined.…”

Section: Resultsmentioning

confidence: 99%

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Sabziparvar¹,

Saeedi²,

Nouri³

et al. 2018

J. Phys. Chem. B

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Nanoparticles (NPs) have received great attention in biological and medical applications because of their unique features. However, their induced adverse effects on the biological system are not well-explored. Herein, the interaction of silicon dioxide nanoparticles (SiO NPs) with human hemoglobin (Hb) and lymphocyte cell line was evaluated under physiological conditions by multispectroscopic [intrinsic and synchronous fluorescence spectroscopy and circular dichrosim (CD)], molecular docking, and cellular [3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) and acridine orange/ethidium bromide (AO/EB) staining] methods. Transmission electron microscopy and dynamic light scattering revealed the nanosized and spherical shaped SiO particle. The fluorescence and lifetime decay results indicated that SiO NPs quenched the intrinsic intensity of Hb through a static quenching mechanism. The binding affinity of SiO NPs toward Hb was directly correlated with temperature. The sign of thermodynamic parameters demonstrated that hydrophobic forces played a pivotal role in the interaction of SiO NPs with Hb. The results of synchronous fluorescence experiments displayed that Tyr residues are moved to a more hydrophilic microenvironment. Molecular docking studies exhibited that SiO and Si NPs were bound to Hb primarily by hydrophobic residues. The findings from CD data verified no alteration in the secondary structure of Hb upon binding to SiO NPs. The human lymphocyte cell line was treated with SiO NPs at varying concentrations and time intervals and the cytotoxicity assays by MTT and AO/EB staining showed that cell viability was reduced by the SiO NP-induced apoptosis mechanism in a dose and time-dependent manner. Therefore, it may be suggested that comprehensive details regarding the interaction of NPs and biological systems such as cells and proteins can provide useful information in the development of NP-based systems.

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Section: Resultsmentioning

confidence: 99%

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Sabziparvar¹,

Saeedi²,

Nouri³

et al. 2018

J. Phys. Chem. B

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“…Increased GSH levels in tissues might indicate an enhancement of GSH biosynthesis to supplement its depletion caused by the formation of Hg-GSH conjugates for detoxification (Guilherme, Válega, Pereira, Santos, & Pacheco, 2008;Monteiro et al, 2010). AHR activity is an important indicator for evaluating the ability to scavenge •OH, the most reactive species of ROS that can react easily with biomolecules (such as amino acids, proteins and DNA) (Tanzadehpanah et al, 2016). Our results showed that Hg 2+ exposure improved the AHR activity in the spleen and head kidney, which might be related to the reinforcement of antioxidant defense mechanisms of immune organs.…”

Section: Discussionmentioning

confidence: 99%

Effects of inorganic mercury exposure on histological structure, antioxidant status and immune response of immune organs in yellow catfish (Pelteobagrus fulvidraco)

Sun

Rao

et al. 2018

J of Applied Toxicology

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Mercury (Hg) is well recognized as a highly toxic substance to fish. Nevertheless, little is known about the toxic effects of Hg on immune organs. In this study, we investigated histology, antioxidant status and immune response of the spleen and head kidney in yellow catfish following 6 weeks of exposure to environmentally relevant concentrations of inorganic Hg (2 and 10 μg l Hg ). As expected, Hg accumulation and histological injury in both tissues were observed. Meanwhile, Hg exposure induced oxidative stress, which increased antioxidant enzyme (superoxide dismutase, catalase and glutathione peroxidase) activities, glutathione content, anti-hydroxyl radical capacity and the expression of genes associated with antioxidant (sod1, cat, gpx1, nrf2 and mt) and stress (hsp70) responses in dose- and tissue-specific manners. In the spleen, the mRNA levels of immune-related genes (il-1β, il-8, tnf-α, il-10, tgf-β, lys and c3) were upregulated by Hg exposure. However, in the head kidney, upregulation of tnf-α, il-10 and tgf-β mRNAs and downregulation of il-1β and lys expressions were observed, while transcriptions of il-8 and c3 were remarkably upregulated only in the 2 μg l group. Overall, our study indicated that Hg exposure could result in Hg accumulation and thereby induced histological impairment, oxidative stress and immunotoxicity in immune organs of yellow catfish, but some enzymes and/or genes involved in antioxidant and immune systems would be activated to resist Hg -induced damage.

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“…The dipeptide VY was also effective in vivo in mild hypertensive subjects (Kawasaki et al, 2000). In addition, the β-casein derived tripeptide HLP and the dipeptide VM are fragments of the ACE-inhibitor peptides LHLP and TEQESGVPVM (Kohmura et al, 1989;Tanzadehpanah et al, 2016).…”

Section: Identification Of Ace-inhibitory and Additional Bioactive Pe...mentioning

confidence: 98%

Angiotensin-converting enzyme inhibitory peptides from goats' milk released by in vitro gastro-intestinal digestion

Tagliazucchi

Shamsia

Helal

et al. 2017

International Dairy Journal

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Bioactive and ACE binding properties of three synthetic peptides assessed by various spectroscopy techniques

Cited by 26 publications

References 34 publications

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Effects of inorganic mercury exposure on histological structure, antioxidant status and immune response of immune organs in yellow catfish (Pelteobagrus fulvidraco)

Angiotensin-converting enzyme inhibitory peptides from goats' milk released by in vitro gastro-intestinal digestion

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