Insect consumption by humans has been encouraged as an alternative to conventional protein sources in light of the scenario predicted by the United Nations, in which the population will reach almost 10 billion people by 2050. It is considered an interesting alternative from the environmental and nutritional points of view. Tenebrio molitor, in the larval stage, is one of the most studied insects in the literature. To expand knowledge about the potential use of insects, in this study an evaluation if the composition of T. molitor and Zophobas morio larvae was evaluated, focusing on the composition of proteins and minerals, and in vitro bioaccessibility of Fe, Ca, Mg, and Zn. T. molitor and Z. morio have the protein as the most abundant component (49.7 g / 100 g for T. molitor and 44.5 g / 100 g for Z. morio), providing the majority of the indispensable amino acids, similarly to foods such as beans and soybeans, with methionine and cysteine as the limiting amino acids. Histidine also appears as a limiting amino acid for Z. morio. The most abundant protein fractions identified by SDS-PAGE were those responsible for melanization. Phosphorus and potassium were the major minerals found, followed by calcium, which showed an amount that exceeds almost three times the calcium content found in beef, pork and chicken, and iron exceeding almost twice the content found in beef. The bioaccessibility of the minerals Ca, Fe, Mg, Zn was assessed by two methods -solubility and dialysis -, which presented different results. When evaluated by solubility, bioaccessibility showed values much higher than dialysis, 43 times higher for Fe. This discrepancy can be attributed to the differences in the methodology. According to the dialysis method results, Mg was the most bioaccessible mineral in both insects, followed by calcium. By solubility, magnesium was the most bioaccessible mineral in T. molitor, and Zn was the most bioaccessible in Z. morio. Both insects can be considered sources of Mg, Zn and Fe, with Mg and Zn having the greatest nutritional potential due to their bioaccessibility.