Bioaccumulation, oxidative stress, and neurotoxicity in <i>Danio rerio</i> exposed to different isotopic compositions of uranium

Barillet, Sabrina; Adam, Christelle; Palluel, Olivier; Devaux, Alain

doi:10.1897/06-243r.1

Cited by 90 publications

(42 citation statements)

References 39 publications

(55 reference statements)

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“…Such results were not expected since other authors such as Kelly and Janz (2009) registered alterations, at least in liver GPx activity in Esox lucius exposed to uranium. Furthermore Barillet et al (2007) also found alterations in CAT and superoxide dismutase activities in Danio rerio exposed to different isotopic compositions of uranium. Nonetheless the biomarker/metal correlation data revealed distinct patterns between livers of animals from both sites, with significantly positive correlations only in the M site, and mainly related with nonessential metals.…”

Section: Discussionmentioning

confidence: 76%

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

et al. 2011

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Human mining activities tend often to generate greatly impacted areas which remain contaminated for long periods of time, giving rise to extreme habitats. Mining sites are usually characterized for the production of metal rich effluents with very low pH. In this work we analyzed physical and chemical parameters of water from a deactivated uranium mine pond (M) and a reference site (REF) as well as their metal content. Furthermore, we determined and compared metal accumulation in liver, kidney, bones, muscle and skin of Pelophylax perezi from REF with P. perezi from M. We also determined the enzymatic activities of glutathione-S-transferases (GSTs), catalase (CAT), glutathione reductase (Gred), and glutathione peroxidase (GPx; both selenium-dependent and selenium-independent) in liver, kidney, lung and heart. Additionally, lipoperoxidation (LPO) was also assessed in the same tissues via thiobarbituric acid reactive substances (TBARS) assay and lactate dehydrogenase (LDH) activity was determined in muscle. Our results revealed that the majority of metals were in higher concentrations in tissues of organisms from M. This trend was especially evident for U whose content reached a difference of 1350 fold between REF and M organisms. None of the organs tested for antioxidant defenses revealed LPO, nonetheless, with exception for liver, all organs from the M frogs presented increased total GPx activity and selenium-dependent GPx. However, this response was significant only for the lung, probably as a consequence of the significant inhibition of CAT upstream and to cope with the subsequent increase in H(2)O(2). Lungs were the organs displaying greater responsiveness of the anti-oxidant stress system in frogs from the uranium mine area.

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Section: Discussionmentioning

confidence: 76%

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

et al. 2011

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“…Uranium is radioactive and the heaviest naturally occurring element that is found in varying but small amounts in soil, rocks, water, plants and animals [6]. Less than 0.1-6% of the uranium is absorbed by the digestive tract, depending on the solubility of the uranium compound [7].…”

Section: Uraniummentioning

confidence: 99%

The transfer and accumulation of trace metals from the Wonderfonteinspruit into the surrounding environment

Hamman

Rensburg

2011

Water and Society

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The Wonderfonteinspruit flows through the richest gold mining region in the world and has subsequently been exposed to the related pollution for more than a century. The issue of pollution in the WFS catchment was raised in 1967 and lingered on with varying intensity ever since, mostly focussing on radioactive pollution. This study attempts to detect and quantify the transfer and accumulation of trace elements and heavy metals from the WFS into the soil, vegetation, water from dams and boreholes and cattle grazing in the area. ICP-MS analysis was used to quantify the differences in the elemental concentrations within the different organs and structures of the cattle and the environment. The possible associations between the elemental concentrations of the water sources, vegetation and that of the soil were determined by using Redundancy analysis. Additional objectives were to assess the water quality of the WFS and the adjacent boreholes for livestock watering purposes. The results illustrate that elements such as uranium, cadmium and chromium, amongst others, are found at levels much higher than the normal levels found in the control group and accumulate in the soil, vegetation and cattle grazing in the area. This suggests that there is a transfer and accumulation of elements from the water into the surrounding environment of the WFS.

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“…The rest of the body (25%), skin, and gonads (20%) contained the highest relative burdens. The other tissues represented a comparatively large mass (from 23 to 40%) and contained organs such as the kidney, spleen, and heart, which may explain the relatively high U burden observed, particularly since the kidney is considered to be a target organ for U accumulation [18]. Uranium accumulation also occurred in other organs (Fig.…”

Section: Accumulationmentioning

confidence: 99%

“…Although data exists on the transfer and effects of U-contaminated water on adult and larvae stages of D. rerio [18,19] (a commonly used biological model in ecotoxicology [20,21]), the effects of U on reproduction and the developing embryo have not yet been studied in fish. Furthermore, dietary exposures have not been examined from the perspective of chemical versus radiological toxicity.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary uranium on reproductive endpoints—fecundity, survival, reproductive success—of the fish Danio rerio

Simon

Mottin

Geffroy

et al. 2010

Enviro Toxic and Chemistry

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Exposure to metal-contaminated water has been shown to result in a number of reproductive abnormalities in adult and larvae fish, such as failure of oocyte maturation and teratogenic effects. Recently, dietary uptake of metals by fish has been recognized as a critical route of exposure, however, the mechanisms of metal uptake and toxicity are poorly understood and in need of further investigation. The objectives of the present study are to quantify uranium (U dietary transfers from spiked artificial diets) in Danio rerio tissues and embryos, as well as establish its effect on reproduction and embryonic development. Uranium's environmental prominence is currently increasing because of new mining and milling activities. Uranium concentrations range from 0.02 µg/L in natural waters to 2 mg/L. The focus of this study was to examine the trophic transfer and effects of U following exposure modalities (dose, exposure duration 1 to 20 d). Two different isotopes were used to distinguish between chemical and radioactivity toxicity of U. Results showed that U trophic transfer was low (0.52%). Uranium tissue distributions showed that accumulation occurred in digestive organs (liver, digestive tract) following dietary exposure. High levels of U were measured in the gonads (female in particular, >20% of relative burden). High U accumulation levels in eggs indicated maternal transfer of the contaminant. Moreover, U trophic exposure led to a reduction in reproduction success as a function of U accumulated levels. High U exposure conditions strongly reduced the total number of eggs (50%) and their viability at 10 d (reduction of the clutch number, low quality of eggs).

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Bioaccumulation, oxidative stress, and neurotoxicity in Danio rerio exposed to different isotopic compositions of uranium

Cited by 90 publications

References 39 publications

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

The transfer and accumulation of trace metals from the Wonderfonteinspruit into the surrounding environment

Effects of dietary uranium on reproductive endpoints—fecundity, survival, reproductive success—of the fish Danio rerio

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