Bioaccumulation of antibiotics and resistance genes in lettuce following cattle manure and digestate fertilization and their effects on soil and phyllosphere microbial communities

Anna, Barra Caracciolo; Visca, Andrea; Rauseo, Jasmin; Spataro, Francesca; Luigi, Garbini Gian; Grenni, Paola; Mariani, Livia; Miritana, Valentina Mazzurco; Massini, Giulia; Patrolecco, Luisa

doi:10.1016/j.envpol.2022.120413

Cited by 10 publications

(9 citation statements)

References 87 publications

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“…Antimicrobial resistance genes are to be naturally present in soil microbiota, but their occurrence is also related to soil contamination by antibiotics. A direct link between antibiotic concentration in soil and a specific gene is difficult to observe given the complexity of natural ecosystems (Caracciolo et al 2022 ). In fact, studies have shown that ARGs can spread or persist even when the antibiotic is fully or partially degraded (Parente et al 2019a , b ; Wang et al 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water

Fučík,

Amrichová,

Brabcová

et al. 2024

Environ Sci Pollut Res

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The practice of incorporating animal manure into soil is supported within the European Circular economy as a possible substitute for mineral fertilizers and will become crucial for the sustainability of agriculture. However, this practice may indirectly contribute to the dissemination of antibiotics, resistance bacteria, and resistance genes. In this study, medicated drinking water and poultry litter samples were obtained from a broiler-chick farm. The obtained poultry litter was incorporated into the soil at the experimental field site. The objectives of this research project were first to develop analytical methods able to quantify fluoroquinolones (FQs) in medicated drinking water, poultry litter, and soil samples by LC–MS; second to study the fate of these FQs in the soil environment after incorporation of poultry litter from flock medicated by enrofloxacin (ENR); and third to screen the occurrence of selected fluoroquinolone resistance encoding genes in poultry litter and soil samples (PCR analysis). FQs were quantified in the broiler farm’s medicated drinking water (41.0 ± 0.3 mg∙L−1 of ENR) and poultry litter (up to 70 mg∙kg−1 of FQs). The persistence of FQs in the soil environment over 112 days was monitored and evaluated (ENR concentrations ranged from 36 μg∙kg−1 to 9 μg∙kg−1 after 100 days). The presence of resistance genes was confirmed in both poultry litter and soil samples, in agreement with the risk assessment for the selection of AMR in soil based on ENR concentrations. This work provides a new, comprehensive perspective on the entry and long-term fate of antimicrobials in the terrestrial environment and their consequences after the incorporation of poultry litter into agricultural fields. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water

Fučík,

Amrichová,

Brabcová

et al. 2024

Environ Sci Pollut Res

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“…The two most abundant phyla in the vegetable roots were Proteobacteria and Actinobacteria , which can promote plant growth and produce secondary metabolites with antimicrobial properties [ 56 ]. The dominant bacterial phyla in vegetable roots were observed to be significantly grown in experiments involving veterinary antibiotics exposure, which are known to contain bacteria with tolerance and degradation capabilities [ 16 , 17 , 18 ]. In addition, Bacteroidetes were abundant in the vegetable roots, which facilitates the degradation of macromolecules, such as starch, cellulose, or proteins [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Antibiotic effects can be attributed to single species such as Aeromonashydrophila , Aeromonassalmonicida , and Ediwardsiellatarda , as well as larger microbial communities such as fungi and bacteria [ 15 , 16 ]. Observations have shown that the percentages of some classes, including Bacilli , Bacteroidia and Gammaproteobacteria , have significantly grown in experiments involving veterinary antibiotics exposure, which are known to contain bacteria with tolerance and degradation capabilities [ 16 , 17 , 18 ]. Proteobacteria and Bacteroidetes , both of which are known to contain antibiotic resistance genes, are often seen to be forming communities that are resistant to antibiotics after a prolonged period of antibiotic usage [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Adaptation of Rhizosphere Microbial Communities to Continuous Exposure to Multiple Residual Antibiotics in Vegetable Farms

Qiu

Chen

Feng

et al. 2023

IJERPH

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The constant application of manure-based fertilizers in vegetable farms leads to antibiotic residue accumulation in soils, which has become a major stressor affecting agroecosystem stability. The present study investigated the adaptation profiles of rhizosphere microbial communities in different vegetable farms to multiple residual antibiotics. Multiple antibiotics, including trimethoprim, sulfonamides, quinolones, tetracyclines, macrolides, lincomycins, and chloramphenicols, were detected in the vegetable farms; the dominant antibiotic (trimethoprim) had a maximum concentration of 36.7 ng/g. Quinolones and tetracyclines were the most prevalent antibiotics in the vegetable farms. The five most abundant phyla in soil samples were Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi and Firmicutes, while the five most abundant phyla in root samples were Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Myxococcota. Macrolides were significantly correlated with microbial community composition changes in soil samples, while sulfonamides were significantly correlated with microbial community composition changes in root samples. Soil properties (total carbon and nitrogen contents and pH) influenced the shifts in microbial communities in rhizosphere soils and roots. This study provides evidence that low residual antibiotic levels in vegetable farms can shift microbial community structures, potentially affecting agroecosystem stability. However, the degree to which the shift occurs could be regulated by environmental factors, such as soil nutrient conditions.

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“…The number of bacteria in fresh black soil samples was determined using the standard dilution plate counting method (Barra Caracciolo et al, 2022). Dilution plate counting was used to count soil fungus in Martin's AGAR medium.…”

Section: Determination Of the Number Of Soil Microorganisms Detectabl...mentioning

confidence: 99%

Effects of microbial inoculants combined with maize straw on physicochemical properties and microbial community structure in black soil during soybean growth

Hou¹,

Yu²,

Du³

et al. 2023

Preprint

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Aim Traditionally, increased crop yield has been supported by applying a large amount of chemical fertilizer. However, using excessive chemical fertilizers on soils diminishes soil fertility and crop yield while contaminating the ecological environment. Method To replace the fertilizer, microbial inoculants, and maize straws were introduced to the soil as environmental fertilizers in this study. During soybean cultivation, their effects on physicochemical properties, enzyme activity, and microbial community structure in the soil were investigated. Compared to fertilization alone, adding microbial inoculants mixed with maize straw (CSF) during soybean cultivation boosted agronomic traits such as soybean fresh weight, plant height, and stem thickness. Result This increased the physicochemical parameters of the soil, such as pH, available Potassium (AP), and water content, affecting the soil bacterial population. Bacteria abundance and diversity significantly increased, with acidobacter, Proteobacteria, Candidatus_ Solibacte, and Sphingomonas dominating. Redundancy analysis revealed that pH and AP were environmental factors that influenced microbial community structure. CSF boosted microbial diversity and community structure while increasing soybean yield from 3.91–5.46%. Conclusion In conclusion, soil improvement using maize straw and composite compound inoculants produced better outcomes. Our findings offer theoretical and experimental references for soil erosion.

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Bioaccumulation of antibiotics and resistance genes in lettuce following cattle manure and digestate fertilization and their effects on soil and phyllosphere microbial communities

Cited by 10 publications

References 87 publications

Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water

Fate of fluoroquinolones in field soil environment after incorporation of poultry litter from a farm with enrofloxacin administration via drinking water

Adaptation of Rhizosphere Microbial Communities to Continuous Exposure to Multiple Residual Antibiotics in Vegetable Farms

Effects of microbial inoculants combined with maize straw on physicochemical properties and microbial community structure in black soil during soybean growth

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