Bioaccumulation and biomarkers in the sea star Asterias rubens (Echinodermata: Asteroidea): a North Sea field study

Besten, P.J. den; Valk, S. de; Weerlee, Evaline van; Nolting, R.F.; Postma, J.F.; Everaarts, J.M.

doi:10.1016/s0141-1136(00)00134-3

Cited by 53 publications

(23 citation statements)

References 27 publications

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“…Everaarts et al 1998, den Besten et al 2001. It is noteworthy that the total PCB#153 concentrations (background + incorporated) reached in the pyloric caeca at the end of our experiments matched the concentrations reported in the same organs of sea stars from moderate to highly contaminated North Sea locations (Table 6).…”

Section: Discussionsupporting

confidence: 85%

“…Being easily dissected and constituting 70 to 80% of the total body weight, the body wall is of particular interest with respect to field surveys, and should be recommended as a body compartment to monitor complementarily to pyloric caeca-the only body compartment used in previous studies (e.g. den Besten et al 1993, 2001, Everaarts et al 1998.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Delineation of PCB uptake pathways in a benthic sea star using a radiolabelled congener

Danis¹,

Cotret²,

Teyssié³

et al. 2003

Mar. Ecol. Prog. Ser.

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Asterias rubens (Linnaeus, 1758), a common sea star in North Sea waters, was selected to study the bioaccumulation of an important polychlorinated biphenyl congener, 14 C-labelled PCB#153, from 2 contrasting sources: seawater and sediments. After 4 wk acclimation to laboratory conditions, sea stars were exposed for 34 d to realistic concentrations (30 ng l -1 in seawater and 9.5 ng g -1 dry wt in sediments) of the contaminant during which time bioaccumulation of PCB#153 was followed in 6 body compartments. The results showed that (1) for each body compartment, PCB uptake kinetics were generally asymptotic and bioaccumulation was far greater when A. rubens was exposed via seawater than via sediments; (2) body wall and podia were the body compartments showing the greatest affinity for the PCB congener, making them ideal tissues for biomonitoring purposes; (3) the concentrations reached in body compartments were within the range of values reported in several field studies. Because radioisotopic techniques are extremely sensitive, they allow key organs that are sometimes too small for standard analysis of PCBs to be taken into account. KEY WORDS: Polychlorinated biphenyls · PCB#153 · Bioaccumulation · Kinetics · Asterias rubens · EchinodermResale or republication not permitted without written consent of the publisher Mar Ecol Prog Ser 253: 155-163, 2003, Everaarts et al. 1998. However, to the best of our knowledge, no study has investigated PCB bioaccumulation processes in A. rubens. The only 2 experimental studies investigating PCB bioaccumulation in echinoderms concern sea urchins exposed to contaminated sediments (Weisberg et al. 1996, Schweitzer et al. 2000, and only Weisberg et al. (1996) examined the kinetic aspects of PCB uptake.Such data are however needed to further assess the value of Asterias rubens as a bioindicator of PCB contamination. Therefore, in the present study, we have investigated the kinetics of PCB uptake in A. rubens exposed either to the contaminant in seawater or associated with sediments, i.e. the 2 extreme pathways of contamination from the viewpoint of absolute PCB concentrations. Indeed, the high hydrophobicity of PCBs result in a characteristic partitioning, with concentrations in natural seawater typically in the range of pg to ng l -1 while sediment concentrations are in the range of µg to mg kg -1 (see Table 1). The PCB congener IUPAC #153 (2, 2', 4, 4', 5, 5' hexachlorobiphenyl) was selected because it is the most abundant in marine biota (Stebbing et al. 1992) and has been shown to be an excellent indicator of total PCB contamination (Atuma et al. 1996). (Linnaeus, 1758) were collected in April 1999 from the intertidal zone at Audresselles (Pas-de-Calais, France). Prior to experimentation, specimens were acclimated to laboratory conditions for 1 mo in constantly aerated closed-circuit aquaria (salinity 36 ‰, T 16 ± 0.5 °C, 12/12 h dark/light cycle). MATERIALS AND METHODS Sampling. Sea stars Asterias rubensIn order to follow PCB#153 bioaccumulation under realistically ...

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Delineation of PCB uptake pathways in a benthic sea star using a radiolabelled congener

Danis¹,

Cotret²,

Teyssié³

et al. 2003

Mar. Ecol. Prog. Ser.

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“…In echinoderms, levels of cadmium in the different tissues (mainly the intestine and gonads) are related to the typically high metabolic activity of these organs. The levels of cadmium found in them tend to increase during their reproduction cycle (Warnau et al, 1995a,b;Den Besten et al, 2001). The concentration of a metal and the way it acts can change depending on the physiological state of the organism and the season that determines the reproduction cycles (Quiniou et al, 1999).…”

Section: Metal Content In the Internal Tissue And Sampling Areasmentioning

confidence: 99%

Accumulation of toxic metals (Pb and Cd) in the sea urchin Diadema aff. antillarum Philippi, 1845, in an oceanic island (Tenerife, Canary Islands)

Hernández

Gutiérrez

González-Weller³

et al. 2010

Environmental Toxicology

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This document shows the results obtained from a study on the concentration of toxic heavy metals in the internal tissue and exoskeleton of sea urchins, collected from their natural habitat. The levels of lead and cadmium were measured by Graphite Furnace Atomic Absorption Spectrometry. The mean concentrations of lead and cadmium in the internal tissue were 304.04 and 260.54 lg/kg respectively, whereas in the shell they were 185.02 and 142.48 lg/kg. We also performed a statistical analysis of the differences in the distribution of metals between their exoskeleton and their internal content, a correlation study of the metal content in internal tissue and shell and sampling areas, and a correlation study between the metal content and sample size. Since the sea urchin Diadema antillarum presents a wide range of variation in metal content, this study suggests that this species is an excellent bioindicator of heavy metal contamination. # 2009 Wiley Periodicals, Inc. Environ Toxicol 25: 227-233, 2010

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“…Regardless the types, ChEs are strongly inhibited by anticholinesterases such as organophosphates (OPs) and cabamates (CBs). This allows them to serve as effective biomarkers in the field (SanchezHernandez et al, 1998;Stien et al, 1998;Kirby et al, 2000;den Besten et al, 2001;Forget et al, 2003;Quintaneiro et al, 2006;Jung et al, 2008;Elhalwagy et al, 2010;Sáenz et al, 2010;van Oosterom et al, 2010;Printes et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Purification and studies on characteristics of cholinesterases from Daphnia magna

Yang

Niu

2013

J. Zhejiang Univ. Sci. B

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Due to their significant value in both economy and ecology, Daphnia had long been employed to investigate in vivo response of cholinesterase (ChE) in anticholinesterase exposures, whereas the type constitution and property of the enzyme remained unclear. A type of ChE was purified from Daphnia magna using a three-step procedure, i.e., Triton X-100 extraction, ammonium sulfate precipitation, and diethylaminoethyl (DEAE)-Sepharose™-Fast-Flow chromatography. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), molecular mass of the purified ChE was estimated to be 84 kDa. Based on substrate studies, the purified enzyme preferred butyrylthiocholine iodide (BTCh) [with maximum velocity (V max )/Michaelis constant (K m )=8.428 L/(min·mg protein)] to acetylthiocholine iodide (ATCh) [with V max /K m =5.346 L/(min·mg protein)] as its substrate. Activity of the purified enzyme was suppressed by high concentrations of either ATCh or BTCh. Inhibitor studies showed that the purified enzyme was more sensitive towards inhibition by tetraisopropylpyrophosphoramide (iso-OMPA) than by 1,5-bis(4-allyldimethylammoniumphenyl) pentan-3-one dibromide (BW284C51). Result of the study suggested that the purified ChE was more like a type of pseudocholinesterase, and it also suggested that Daphnia magna contained multiple types of ChE in their bodies.

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Bioaccumulation and biomarkers in the sea star Asterias rubens (Echinodermata: Asteroidea): a North Sea field study

Cited by 53 publications

References 27 publications

Delineation of PCB uptake pathways in a benthic sea star using a radiolabelled congener

Delineation of PCB uptake pathways in a benthic sea star using a radiolabelled congener

Accumulation of toxic metals (Pb and Cd) in the sea urchin Diadema aff. antillarum Philippi, 1845, in an oceanic island (Tenerife, Canary Islands)

Purification and studies on characteristics of cholinesterases from Daphnia magna

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