Bioaccessibility, safety, and antidiabetic effect of phenolic-rich extract from fermented Psidium guajava Linn. leaves

Huang, Zhenfeng; Luo, You; Xia, Xuesen; Wu, Anping; Wu, Zhenqiang

doi:10.1016/j.jff.2021.104723

Cited by 13 publications

(7 citation statements)

References 61 publications

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“…The identified compounds as shown in Figure 1 were assorted into different chemical classes. Identification of the compounds was achieved based on HR-ESIMS data and comparison with literature data as follows: Cis-3-Hexenyl isobutyrate (1) [37], cinnamyl acetate (2) [38], coumaric acid (3) [39], β-sitosterol (4) [39], α-tocopherol (5) [39], linoleic acid (6) [39], palmitic acid (7) [39], stearic acid (8) [39], oleic acid (9) [39], linolenic acid (10) [39], vanillic acid (11) [39], 4-Hydroxybenzaldehyde (12) [39], vanillin (13) [39], syringaldehyde (14) [39], coniferylaldehyde (15) [39], sinapaldehyde (16) [39], abscisic acid (17) [39], cinnamic acid (18) [39], cinnamaldehyde (19) [39], campesterol (20) [39], stigmastanol (21) [39], stigmasterol (22) [39] and quercetin 4 -glucuronide (23) [40]. Guava seed extract provided substantial protection against the ulcerogenic effects of indomethacin in a dose-dependent manner (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Guava leaves and bark were assigned to treat gastrointestinal disorders; particularly diarrhea [ 11 ]. In the last decades, several studies were performed using the decocted or infused extracts of leaves, fruits, stems, and flower buds to study their different biological activities [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. The anti-inflammatory, antihypertensive, blood glucose lowering effect, antiviral and cough sedative activities are the key factors which labelled the guava fruit to be a perfect candidate to protect against COVID-19 infection especially for chronic diseased individuals [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Antiulcer Potential of Psidium guajava Seed Extract Supported by Metabolic Profiling and Molecular Docking

et al. 2022

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One of the most severe human health problems is gastric ulceration. The main aim of our study is to explore the gastroprotective effect of the Psidium guajava seeds extract (PGE). Metabolic profiling based on LC-HRMS for the extract led to the dereplication of 23 compounds (1–23). We carried out a gastric ulcer model induced by indomethacin in male albino rats in vivo and the extract of PGE was investigated at a dose of 300 mg/kg in comparison to cimetidine (100 mg/kg). Furthermore, the assessment of gastric mucosal lesions and histopathology investigation of gastric tissue was done. It has been proved that Psidium guajava seeds significantly decreased the ulcer index and protected the mucosa from lesions. The antiulcer effect of Psidium guajava seed extract, which has the power of reducing the ensuing inflammatory reactions, can counteract the inflammation induced by indomethacin by the downregulation of relative genes expression (IL-1β, IL-6, and TNF-α). Moreover, PGE significantly downregulated the increased COX-2, TGF-β, and IGF-1 relative genes expression, confirming its beneficial effect in ulcer healing. Moreover, the possible PGE antioxidant potential was determined by in vitro assays using hydrogen peroxide and superoxide radical scavenging and revealed high antioxidant potential. Additionally, on the putatively annotated metabolites, an in silico study was conducted, which emphasized the extract’s antiulcer properties might be attributed to several sterols such as stigmasterol and campesterol. The present study provided evidence of Psidium guajava seeds considered as a potential natural gastroprotective agent.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiulcer Potential of Psidium guajava Seed Extract Supported by Metabolic Profiling and Molecular Docking

et al. 2022

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“…Flavonoid glycoside, isoquercetin exhibited more α ‐glucosidase inhibitory activity than flavonoid galactoside, hyperoside and flavonoid rhamnoside, quercetin (Flores‐Bocanegra et al, 2015), which was also illustrated in this study. Avicularin showed good inhibitory effect against α ‐glucosidase enzyme (Huang et al, 2021; Tasnuva et al, 2017). Flavonoid galactoside was more active than flavonoid galactoside, hyperoside in the current study.…”

Section: Resultsmentioning

confidence: 99%

Rapid screening of α‐glucosidase inhibitors in Hypericum perforatum L. using bio‐affinity chromatography coupled with UPLC/MS

Dong

Yue

et al. 2022

Biomedical Chromatography

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α‐glucosidase inhibitors (AGIs) are widely used for the treatment of type 2 diabetes, but their side effects have made it to develop novel and alternative AGIs immediately. In this study, the extract of Hypericum perforatum L. (HPE) has been confirmed to have α‐glucosidase inhibitory activity in vitro and in vivo. Seven active compounds, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, quercetin, and biapigenin, were screened based on a bio‐affinity chromatography column with α‐glucosidase enzyme‐conjugated solid phase and UPLC/MS, which exhibited excellent α‐glycosidase inhibitory effects by the determined IC50 values. The mechanism of α‐glycosidase inhibitory activity of biapigenin was studied for the first time. The results showed that biapigenin was a high‐potential, reversible, and mixed enzyme inhibitor. Analysis by molecular docking further revealed that hydrophobic interactions were generated by interactions between biapigenin and amino acid residues LYS156, PHE303, PHE314, and LEU313. In addition, hydrogen bonding occurred between biapigenin and α‐glucosidase amino acid residues ASP307, SER241, and LYS156. This research identified that biapigenin could be a novel AGI and further applied to the development of potential anti‐diabetic drugs. Furthermore, our studies established a rapid in vitro screening method for AGIs from plants.

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“…The decrease of phenolic compounds in gastrointestinal digestion can be attributed to degradation, interaction with other compounds or to precipitation formation by binding with enzymes during the digestion process, as they can combine with digestive enzymes such as pepsin, for example, which decreases their bioaccessibility (Huang et al, 2021). In addition, polyphenols are bound to macromolecular compounds that are not dialyzable, and can form mineral complexes, further reducing their solubility (Silva et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Cold extraction of minimally processed Stevia UEM‐13 leaves: Amylase inhibition and increased bioaccessibility of bioactive compounds by microencapsulation

Zorzenon

Cabeça

Nogueira

et al. 2022

Food Processing Preservation

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Sweeteners and other bioactives present in the leaves of Stevia rebaudiana have shown functional and nutritional benefits in several pathologies. The number of consumers of teas and plant extracts has grown significantly, but little is known about cold extraction, especially of Stevia. Thus, the objectives of this study were to perform a semi-continuous cold percolation extraction of minimally processed Stevia UEM-13 leaves and to microencapsulate this extract with maltodextrin by Spray Dryer, in order to evaluate possible functional effects of the free and microencapsulated extract. The extract had 25.27 g/100 g of steviol glycosides, quantified by HPLC (11.9% rebaudioside A and 10.2% stevioside) and 72.36 μgGAE/mg of total phenolic compounds. The antioxidant capacity was 50.2% for DPPH radical and 28.4% for ABTS •+ .The microencapsulation presented efficiency of 70.59%, with well-defined spheres by visualization through scanning electron microscopy. The free and microencapsulated extract (0.5 mg/ml) decreased the enzymatic activity of α-amylase with possible noncompetitive or mixed inhibition in a dose-independent manner and also showed good acceptance in the preliminary sensory test with little bitter and herbaceous residual.The bioaccessibility of bioactive compounds (sweeteners, total phenolics, antioxidant activity for DPPH and ABTS •+ radicals) was evaluated by simulating in vitro digestion and those that were microencapsulated showed higher bioaccessibility for all conditions (mouth, gastric, and intestinal conditions). Thus, it is suggested the direct use or application in food of the microencapsulated extract obtained in cold of Stevia leaves, for the preservation of bioactive compounds and possible functional activities. Novelty impact statement:• Cold extraction of Stevia leaves shows similar yields to hot extraction and contains significant contents of phenolic compounds and antioxidant activity.• The cold extract 0.5 mg/ml contains possible antidiabetic property presenting α-amylase enzyme inhibition.• The microencapsulation of the crude stevia extract preserves the bioactive compounds and antioxidant activity on simulated in vitro digestion.

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Bioaccessibility, safety, and antidiabetic effect of phenolic-rich extract from fermented Psidium guajava Linn. leaves

Cited by 13 publications

References 61 publications

Antiulcer Potential of Psidium guajava Seed Extract Supported by Metabolic Profiling and Molecular Docking

Antiulcer Potential of Psidium guajava Seed Extract Supported by Metabolic Profiling and Molecular Docking

Rapid screening of α‐glucosidase inhibitors in Hypericum perforatum L. using bio‐affinity chromatography coupled with UPLC/MS

Cold extraction of minimally processed Stevia UEM‐13 leaves: Amylase inhibition and increased bioaccessibility of bioactive compounds by microencapsulation

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