Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach

Alves, Ricardo; Rambla-Alegre, Maria; Braga, Ana Catarina; Maulvault, Ana Luísa; Barbosa, Vera; Campàs, Mònica; Reverté, Laia; Flores, Cintia; Caixach, Josep; Kilcoyne, Jane; Costa, Pedro Reis; Diogène, Jorge; Marques, António

doi:10.1016/j.fct.2019.04.041

Cited by 22 publications

(24 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the surveillance plan conducted from January 2016 to December 2019, less than 1% of all samples resulted contaminated by lipophilic marine biotoxins belonging to the OA-group at concentrations ranging from 25.6 to 87.0 µg/kg, and 65% of samples positive only to OA showed this compound in the esteri ed form (Table 6). It is well known that both OA and DTX2 can be found in free or esteri ed forms (Alves et al 2019) and the different proportion of them could arise from the genetic variability of bivalve molluscs, since the OA esteri cation is an enzymatic mechanism associated with the detoxi cation process (Prassopoulou et al 2009). OA was found to be present at higher concentrations in the hydrolysed extracts, with relative proportions of free and esteri ed form showing the dominance of the last.…”

Section: Resultsmentioning

confidence: 99%

“…They are produced by harmful algal blooms due to climate and sea surface temperature variations (Young et al 2020) and are distinguished in hydrophilic and lipophilic marine biotoxins (Schirone et al 2011). Many different compounds belong to the last group, such as okadaic acid (OA) and its analogues dinophysistoxins (DTX1, DTX2 and DTX3), that are the most common in the European countries (Alves et al 2019) and together with azaspiracids (AZA1, AZA2 and AZA3) cause the diarrhetic shell sh poisoning (DSP) outbreaks, while pectenotoxins (PTXs) and yessotoxins (YTXs) are not proven to provoke such symptoms after their ingestion (Hassoun et al 2021). The main symptoms of OA intoxication are nausea, vomiting, abdominal pain and diarrhea appearing within 30 min up to few hours and declining generally after 3 days (Dietrich et al 2019).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Surveillance Plan of Lipophilic Marine Biotoxins in Molluscs from the Croatian Coast

et al. 2021

View full text Add to dashboard Cite

In the present study, a 4-year surveillance plan for the detection of lipophilic marine biotoxins in Mytilus galloprovincialis and Callista chione from different aquaculture farms and natural beds along the Central Adriatic coasts of Croatia was reported. The samples were analyzed by a validated LC-MS/MS method in accordance with the Regulation (EU) No 2019/627. Lipophilic marine biotoxins belonging to the okadaic acid group, except for azaspiracids, as well as yessotoxins were found at different concentrations, always below the maximum limits established by the Regulation (EC) No 853/2004. The seasonal distribution showed the highest values in summer months, and an interannual variability was also observed, probably due to environmental conditions aging on the density of harmful algal blooms. The comprehensive data reported in this study should help the future research in making advancing prediction models along the Eastern Adriatic coast.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surveillance Plan of Lipophilic Marine Biotoxins in Molluscs from the Croatian Coast

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The difference between the in vitro simulated digestion model and the existing static in vitro model is mainly reflected in the pH of gastric phase. The importance of pH values with in vitro simulated digestion in the bioaccessibility has been previously suggested (51,52). Gastric pH is not a constant parameter and its value changes continuously during digestion (37).…”

Section: Del Bioaccessibility and Changes After In Vitro Digestion And Cell Monolayersmentioning

confidence: 99%

Bioaccessibility and Intestinal Transport of Deltamethrin in Pacific Oyster (Magallana Gigas) Using Simulated Digestion/NCM460 Cell Models

et al. 2021

View full text Add to dashboard Cite

Deltamethrin (DEL) can be introduced into the food chain through bioaccumulation in Pacific oysters, and then potentially threaten human health. The objective of this study was to investigate the bioaccessibility of DEL in oysters with different cooking methods after simulated digestion. DEL content in different tissues of oysters going from high to low were gills, mantle, viscera, and adductor muscle. Bioaccessibility of DEL in oysters decreased after steaming (65%) or roasting (51%) treatments compared with raw oysters (82%), which indicated that roasting can be used as a recommended cooking method for oysters. In the simulated digestion process, the concentration of DEL in the digestive juice and the bioaccessibility of DEL were affected by the pH in the gastric phase. And the transport efficiency of DEL through the monolayer molecular membrane of NCM460 cells ranged from 35 to 45%. These results can help assess the potential harm to consumers of DEL in shellfish. Furthermore, it provides a reference for the impact of lipophilic toxins in seafood.

show abstract

“…Most relevant research topics include DSTs mechanisms of action, molecular and cellular targets and toxicokinectics. Other relevant research topics are the transfer of DSTs in the food chain and DSTs bioaccessibility and bioavailability [ 16 , 17 ]. Most of the studies carried out in these topics are contributing to improve the prevention and/or mitigation of the adverse effects of DSTs in human health, environment and aquaculture and fisheries.…”

Section: Introductionmentioning

confidence: 99%

OMICs Approaches in Diarrhetic Shellfish Toxins Research

Campos

Freitas

Almeida

et al. 2020

Toxins

Self Cite

View full text Add to dashboard Cite

Diarrhetic shellfish toxins (DSTs) are among the most prevalent marine toxins in Europe’s and in other temperate coastal regions. These toxins are produced by several dinoflagellate species; however, the contamination of the marine trophic chain is often attributed to species of the genus Dinophysis. This group of toxins, constituted by okadaic acid (OA) and analogous molecules (dinophysistoxins, DTXs), are highly harmful to humans, causing severe poisoning symptoms caused by the ingestion of contaminated seafood. Knowledge on the mode of action and toxicology of OA and the chemical characterization and accumulation of DSTs in seafood species (bivalves, gastropods and crustaceans) has significantly contributed to understand the impacts of these toxins in humans. Considerable information is however missing, particularly at the molecular and metabolic levels involving toxin uptake, distribution, compartmentalization and biotransformation and the interaction of DSTs with aquatic organisms. Recent contributions to the knowledge of DSTs arise from transcriptomics and proteomics research. Indeed, OMICs constitute a research field dedicated to the systematic analysis on the organisms’ metabolisms. The methodologies used in OMICs are also highly effective to identify critical metabolic pathways affecting the physiology of the organisms. In this review, we analyze the main contributions provided so far by OMICs to DSTs research and discuss the prospects of OMICs with regard to the DSTs toxicology and the significance of these toxins to public health, food safety and aquaculture.

show abstract

Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach

Cited by 22 publications

References 97 publications

Surveillance Plan of Lipophilic Marine Biotoxins in Molluscs from the Croatian Coast

Surveillance Plan of Lipophilic Marine Biotoxins in Molluscs from the Croatian Coast

Bioaccessibility and Intestinal Transport of Deltamethrin in Pacific Oyster (Magallana Gigas) Using Simulated Digestion/NCM460 Cell Models

OMICs Approaches in Diarrhetic Shellfish Toxins Research

Contact Info

Product

Resources

About