Bio-vaterite formation by glycoproteins from freshwater pearls

Natoli, Antonino; Wiens, Matthias; Schröder, Heinz C.; Štifanić, Mauro; Batel, Renato; Soldati, Analía Leticia; Jacob, Dorrit E.; Müller, Wernér E.G.

doi:10.1016/j.micron.2010.01.002

Cited by 43 publications

(29 citation statements)

References 50 publications

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“…These studies were in vitro in nature and utilized corresponding calcium carbonate mineralization assays as described for aragonite studies [48,52,53]. Again, as per the aragonite studies, these studies confirmed the formation of metastable vaterite crystals in the presence of the proteins under investigation [48,52,53].…”

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 63%

“…In comparison to the aragonite studies, there have been fewer published studies that examine the role of proteins in metastable vaterite formation [48,52,53], most likely because of fewer organisms utilizing this polymorph over calcite or aragonite [52,53,57]. These studies were in vitro in nature and utilized corresponding calcium carbonate mineralization assays as described for aragonite studies [48,52,53].…”

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

“…The major reason for this is the lack of standardization: even though conditions for studying non-classical nucleation schemes were published nearly 8 years ago [30] there are few examples in the protein literature that utilize these methods of generating ion supersaturation and subsequent nucleation. Further, many studies relied on different ion concentration levels, control conditions, time periods, temperatures, pH values, and most importantly, protein quantities, in their assay experiments [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53]. Typically, in most cases protein quantities have been reported as mass/volume values [34][35][36][37][38][39][40][41][42][43][44][45]52,53] rather than mole values [46][47][48][49][50][51], and this lack of standardization prevents cross-comparisons between protein studies.…”

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

“…Second, and perhaps most importantly, genomic and proteomic studies of biomineralization proteins have uncovered a vast repertory of proteins [6][7][8]13,14,[26][27][28] that could potentially manage many aspects of proposed nucleation processes, including polymorphism. However, although many protein studies have confirmed that polymorphs do form when proteins are present [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53], these studies have not provided information that is needed for detailing the mechanisms of polymorph formation, or, have utilized a variety of testing methods that prevent cross-comparisons of datasets necessary for mechanism building [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]…”

Section: Introductionmentioning

confidence: 99%

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Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

Evans

2017

Minerals

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Over the last eight years new theories regarding nucleation, crystal growth, and polymorphism have emerged. Many of these theories were developed in response to observations in nature, where classical nucleation theory failed to account for amorphous mineral precursors, phases, and particle assembly processes that are responsible for the formation of invertebrate mineralized skeletal elements, such as the mollusk shell nacre layer (aragonite polymorph) and the sea urchin spicule (calcite polymorph). Here, we summarize these existing nucleation theories and place them within the context of what we know about biomineralization proteins, which are likely participants in the management of mineral precursor formation, stabilization, and assembly into polymorphs. With few exceptions, much of the protein literature confirms that polymorph-specific proteins, such as those from mollusk shell nacre aragonite, can promote polymorph formation. However, past studies fail to provide important mechanistic insights into this process, owing to variations in techniques, methodologies, and the lack of standardization in mineral assay experimentation. We propose that the way forward past this roadblock is for the protein community to adopt standardized nucleation assays and approaches that are compatible with current and emerging nucleation precursor studies. This will allow cross-comparisons, kinetic observations, and hopefully provide the information that will explain how proteins manage polymorph formation and stabilization.

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Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 63%

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

Evans

2017

Minerals

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“…Moreover, a comparison of the microstructure and composition of aragonite and vaterite pearls with the nacre layer of H. cumingii shells revealed that vaterite tablets are packed with a greater number of organic matrices and exhibited a more irregular texture compared to that of aragonite tablets . A 48-kDa acidic and putative calcium-binding glycoprotein, believed to affect crystal growth, was isolated and characterized from H. cumingii pearls (Natoli et al, 2010). The recently cloned nacrein gene reportedly codes for a protein that could accelerate the growth of prismatic-shaped crystals by influencing the secretion of mantle tissue (Han et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a novel acidic matrix protein hic22 from the nacreous layer of the freshwater mussel, Hyriopsis cumingii

Liu

Jin

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Matrix proteins that either weakly acidic or unusually highly acidic have important roles in shell biomineralization. In this study, we have identified and characterized hic22, a weakly acidic matrix protein, from the nacreous layer of Hyriopsis cumingii. Total protein was extracted from the nacre using 5 M EDTA and hic22 was purified using a DEAE-sepharose column. The N-terminal amino acid sequence of hic22 was determined and the complete cDNA encoding hic22 was cloned and sequenced by rapid amplification of cDNA endspolymerase chain reaction. Finally, the localization and distribution of hic22 was determined by in situ hybridization. Our results revealed that 2 X.J. Liu et al. Genetics and Molecular Research 15 (3): gmr.15038656 hic22 encodes a 22-kDa protein composed of 185 amino acids. Tissue expression analysis and in situ hybridization indicated that hic22 is expressed in the dorsal epithelial cells of the mantle pallial; moreover, significant expression levels of hic22 were observed after the early formation of the pearl sac (days 19-77), implying that hic22 may play an important role in biomineralization of the nacreous layer.

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Stimulation by Pearl of Mineralization and Biocompatibility of PLA

et al. 2015

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Biodegradable materials used for bone repair have recently gained greater attention. The improvement of the mineralization rate and the biocompatibility of are critical. In this study, natural pearl powder is added to polylactic acid (PLA) to accelerate both two properties of PLA. The results show that the amount of hydroxyapatite (HA) deposition on PLA/pearl composite increased with pearl content increasing up to 15 wt%, especially at the beginning of mineralization. The HA is a poorly crystallized calcium-deficient type as indicated by FTIR, EDS, and XRD. Osteoblasts are used to evaluate cellular proliferation. An MTT assay reveals that composites are non-toxic and show better proliferation than pristine PLA. It is concluded that PLA/pearl could be more appropriate for bone repairs than PLA.

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Bio-vaterite formation by glycoproteins from freshwater pearls

Cited by 43 publications

References 50 publications

Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

Isolation and characterization of a novel acidic matrix protein hic22 from the nacreous layer of the freshwater mussel, Hyriopsis cumingii

Stimulation by Pearl of Mineralization and Biocompatibility of PLA

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