Bio-removal of PtCl62− complex by Galdieria sulphuraria

Sun, Yabo; Shi, Menghan; Lü, Tao; Ding, Dawei; Sun, Yingqiang; Yuan, Yupeng

doi:10.1016/j.scitotenv.2021.149021

Cited by 17 publications

(5 citation statements)

References 52 publications

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“…Despite having a rigid cell wall, G. sulphuraria has been reported to retain the ability to form an extracellular mucilage matrix, although possibly at very low amounts and not always (Gaignard et al, 2019; Lang et al, 2020; Oesterhelt et al, 2008; Vis & Necchi, 2021; Weber et al, 2007). In fact, EPSs have been only recently measured in this species and their functions have not been investigated yet (Sun et al, 2021; Zhu et al, 2022). In other photosynthetic microorganisms, EPSs serve a plethora of functions, including providing structural support, external nutrient storage, or protection against environmental stresses (Flemming & Wingender, 2010).…”

Section: Resultsmentioning

confidence: 99%

Scale‐down of oxygen and glucose fluctuations in a tubular photobioreactor operated under oxygen‐balanced mixotrophy

Fernández

García

Jansen

et al. 2023

Biotech & Bioengineering

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Oxygen-balanced mixotrophy (OBM) is a novel type of microalgal cultivation that improves autotrophic productivity while reducing aeration costs and achieving high biomass yields on substrate. The scale-up of this process is not straightforward, as nonideal mixing in large photobioreactors might have unwanted effects in cell physiology. We simulated at lab scale dissolved oxygen and glucose fluctuations in a tubular photobioreactor operated under OBM where glucose is injected at the beginning of the tubular section. We ran repeated batch experiments with the strain Galdieria sulphuraria ACUF 064 under glucose pulse feeding of different lengths, representing different retention times: 112, 71, and 21 min. During the long and medium tube retention time simulations, dissolved oxygen was depleted 15-25 min after every glucose pulse. These periods of oxygen limitation resulted in the accumulation of coproporphyrin III in the supernatant, an indication of disruption in the chlorophyll synthesis pathway. Accordingly, the absorption cross-section of the cultures decreased steeply, going from values of 150-180 m 2 kg −1 at the end of the first batch down to 50-70 m 2 kg −1 in the last batches of both conditions. In the short tube retention time simulation, dissolved oxygen always stayed above 10% air saturation and no pigment reduction nor coproporphyrin III accumulation were observed. Concerning glucose utilization efficiency, glucose pulse feeding caused a reduction of biomass yield on substrate in the range of 4%-22% compared to the maximum levels previously obtained with continuous glucose feeding (0.9 C-g C-g −1 ).The missing carbon was excreted to the supernatant as extracellular polymeric substances constituted by carbohydrates and proteins. Overall, the results point out the importance of studying large-scale conditions in a controlled environment and the need for a highly controlled glucose feeding strategy in the scale-up of mixotrophic cultivation.

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Section: Resultsmentioning

confidence: 99%

Scale‐down of oxygen and glucose fluctuations in a tubular photobioreactor operated under oxygen‐balanced mixotrophy

Fernández

García

Jansen

et al. 2023

Biotech & Bioengineering

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“…Other studies reported that this alga could also be used in metal removal, such as for gold, rare elements, palladium, and negative charged platinum (PtCl 6 2− ). Moreover, it is a good candidate for producing bio-oils through hydrothermal liquefaction [41][42][43][44][45]. Nevertheless, nothing has been reported on its antiviral properties.…”

Section: Discussionmentioning

confidence: 99%

Galdieria sulphuraria: An Extremophilic Alga as a Source of Antiviral Bioactive Compounds

et al. 2023

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In the last decades, the interest in bioactive compounds derived from natural sources including bacteria, fungi, plants, and algae has significantly increased. It is well-known that aquatic or terrestrial organisms can produce, in special conditions, secondary metabolites with a wide range of biological properties, such as anticancer, antioxidant, anti-inflammatory, and antimicrobial activities. In this study, we focused on the extremophilic microalga Galdieria sulphuraria as a possible producer of bioactive compounds with antiviral activity. The algal culture was subjected to organic extraction with acetone. The cytotoxicity effect of the extract was evaluated by the 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The antiviral activity was assessed through a plaque assay against herpesviruses and coronaviruses as enveloped viruses and poliovirus as a naked one. The monolayer was treated with different concentrations of extract, ranging from 1 µg/mL to 200 µg/mL, and infected with viruses. The algal extract displayed strong antiviral activity at non-toxic concentrations against all tested enveloped viruses, in particular in the virus pre-treatment against HSV-2 and HCoV-229E, with IC50 values of 1.7 µg/mL and IC90 of 1.8 µg/mL, respectively. However, no activity against the non-enveloped poliovirus has been detected. The inhibitory effect of the algal extract was confirmed by the quantitative RT-PCR of viral genes. Preliminary chemical profiling of the extract was performed using ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS), revealing the enrichment in primary fatty acid amides (PFAA), such as oleamide, palmitamide, and pheophorbide A. These promising results pave the way for the further purification of the mixture to explore its potential role as an antiviral agent.

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“…30 mL of methanol (≥99.5%, Xilong Scientific, Guangdong, China) was then added into the mixture and magnetically stirred for 30 min. Afterward, 15 mL of chloroform and an equivalent amount of distilled water were added and stirred for another 30 min. , The mixture obtained was filtrated with a filter cake washed with certain amount of chloroform three times, ensuring all lipids were extracted out. The filtrate was poured out and placed in a separatory funnel for liquid separation.…”

Section: Methodsmentioning

confidence: 99%

Competition of Dual Roles of Ionic Liquids during In Situ Transesterification of Wet Algae

Ding

Shen

et al. 2022

ACS Sustainable Chem. Eng.

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Ionic liquid (IL)-catalyzed in situ transesterification (IST) of wet algae is a promising strategy for energy-efficient biodiesel production owing to the dual roles of ILs as both solvents of the cell wall and catalysts of transesterification, while their variations and interactions within different water contents, which subsequently affect biodiesel production, are still out of knowledge. Accordingly, the variations of fatty acid methyl ester (FAME) yields and cellulose solubilities of [Bmim][HSO 4 ], [Bmim]-[H 2 PO 4 ], and [Bmim] 2 [HPO 4 ] under different water conditions are experimentally and theoretically studied. Results indicate that [Bmim][HSO 4 ], [Bmim][H 2 PO 4 ], and [Bmim] 2 [HPO 4 ] are acid, weak alkaline, and strong alkaline catalysts of transesterification of lipid with methanol, respectively. FAME yields of both [Bmim]-[HSO 4 ]-and [Bmim][H 2 PO 4 ]-catalyzed reactions increase initially with the addition of 1.5 g of water, while they decrease with the increase of water content because of the consequent decline of the nucleophilic index and electrophilic index of the OH groups of [Bmim][HSO 4 ] and [Bmim][H 2 PO 4 ], respectively. Analogously, slight decreases of FAME yields of [Bmim] 2 [HPO 4 ]-catalyzed transesterification are observed within 0.0−3.0 g of water, while a notable decrease is presented when 6.0 g of water is added. Contrary to the variation of catalytic properties, the cellulose solubilities of [Bmim][HSO 4 ], [Bmim][H 2 PO 4 ], and [Bmim] 2 [HPO 4 ] all decrease with the addition of 1.5 g of water, as the water molecules occupy the H-bonding sites of [Bmim][HSO 4 ]−cellobiose (clb), while the H-bonding interactions of water−clb and water− [Bmim][H 2 PO 4 ] formed by shared oxygen atoms are enhanced with the increase of water content, subsequently enhancing the Hbonding interactions of ILs−clb and relative cellulose solubilities. The variations of cellulose solubilities of ILs with the addition of 0.0−6.0 g of water are opposite to those of their catalytic properties. This study indicates that the cellulose solubilities and catalytic properties of [Bmim][HSO 4 ], [Bmim][H 2 PO 4 ], and [Bmim] 2 [HPO 4 ] compete with each other under different water conditions, providing a potential strategy to offset the negative effect of water content on IST of wet algae.

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Bio-removal of PtCl62− complex by Galdieria sulphuraria

Cited by 17 publications

References 52 publications

Scale‐down of oxygen and glucose fluctuations in a tubular photobioreactor operated under oxygen‐balanced mixotrophy

Scale‐down of oxygen and glucose fluctuations in a tubular photobioreactor operated under oxygen‐balanced mixotrophy

Galdieria sulphuraria: An Extremophilic Alga as a Source of Antiviral Bioactive Compounds

Competition of Dual Roles of Ionic Liquids during In Situ Transesterification of Wet Algae

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