Bio-production of gaseous alkenes: ethylene, isoprene, isobutene

Wilson, James N.; Gering, Sarah; Pinard, Jessica; Lucas, Ryan; Briggs, Brandon R.

doi:10.1186/s13068-018-1230-9

Cited by 21 publications

(13 citation statements)

References 102 publications

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“…It has several advantages such as a higher energy density, compatibility with existing engines, lower vapor pressure and volatility, as well as a lower corrosivity compared to bio‐ethanol . Furthermore, isobutanol is used in the chemical industry and can be used to produce the gaseous alkene precursor isobutene .…”

Section: Introductionmentioning

confidence: 99%

Engineering Pseudomonas putida KT2440 for the production of isobutanol

Nitschel

Ankenbauer

Welsch

et al. 2020

Engineering in Life Sciences

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We engineered P. putida for the production of isobutanol from glucose by preventing product and precursor degradation, inactivation of the soluble transhydrogenase SthA, overexpression of the native ilvC and ilvD genes, and implementation of the feedbackresistant acetolactate synthase AlsS from Bacillus subtilis, ketoacid decarboxylase KivD from Lactococcus lactis, and aldehyde dehydrogenase YqhD from Escherichia coli. The resulting strain P. putida Iso2 produced isobutanol with a substrate specific product yield (Y Iso/S ) of 22 ± 2 mg per gram of glucose under aerobic conditions. Furthermore, we identified the ketoacid decarboxylase from Carnobacterium maltaromaticum to be a suitable alternative for isobutanol production, since replacement of kivD from L. lactis in P. putida Iso2 by the variant from C. maltaromaticum yielded an identical Y Iso/S . Although P. putida is regarded as obligate aerobic, we show that under oxygen deprivation conditions this bacterium does not grow, remains metabolically active, and that engineered producer strains secreted isobutanol also under the non-growing conditions. K E Y W O R D Sisobutanol, ketoacid decarboxylase, metabolic engineering, microaerobic, Pseudomonas putida Abbreviations: 2-KIV, 2-ketoisovalerate; AlsS, acetolactate synthase; BHI, brain-heart infusion; KDC, ketoacid decarboxylase; LB, Lysogeny broth.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Introductionmentioning

confidence: 99%

Engineering Pseudomonas putida KT2440 for the production of isobutanol

Nitschel

Ankenbauer

Welsch

et al. 2020

Engineering in Life Sciences

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“…However, extensive metabolic engineering is necessary to achieve viable productivity, with the best throughput to date being 34 mg L −1 h −1 , whereas 2–4 g L −1 h −1 would be required to be economically feasible. [ 98 ]…”

Section: Production Of Biobased Acrylates and Analogsmentioning

confidence: 99%

Production and Polymerization of Biobased Acrylates and Analogs

Fouilloux

Thomas

2021

Macromol. Rapid Commun.

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To prepare biobased polymers, particular attention must be paid to the obtention of the monomers from which they are derived. (Meth)acrylates and their analogs constitute such a class of monomers that have been extensively studied due to the wide range of polymers accessible from them. This review therefore aims to highlight the progresses made in the production and polymerization of (meth)acrylates and their analogs. Acrylic acid production from biomass is close to commercialization, as three different high‐potential intermediates are identified: glycerol, lactic acid, and 3‐hydroxypropionic acid. Biobased methacrylic acid is less common, but several promising options are available, such as the decarboxylation of itaconic acid or the dehydration of 2‐hydroxyisobutyric acid. Itaconic acid is also a vinylic monomer of great interest, and polymers derived from it have already found commercial applications. Methylene butyrolactones are promising monomers, obtained from bioresources via three different intermediates: levulinic, succinic, or itaconic acid. Although expensive, methylene butyrolactones have a strong potential for the production of high‐performance polymers. Finally, β‐substituted acrylic monomers, such as cinnamic, fumaric, muconic, or crotonic acid, are also examined, as they provide an original access to biobased materials from various renewable raw materials, such as protein waste, lignin, or wastewater.

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“…Интересно отметить, что многие растения и микроорганизмы имеют биохимические пути синтеза углеводородов из сахаров. Эти пути могут быть улучшены методами метаболической инженерии [1]. Все же, несмотря на усилия ученых и инженеров, уровень продукции биоалканов остается достаточно низким для создания прямой конкуренции алканам, получаемым из нефти и газа.…”

Section: биотехнологический изобутилен продолжает путь к рынкуunclassified

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2019

Biiotehnologiâ (Mosk.)

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Bio-production of gaseous alkenes: ethylene, isoprene, isobutene

Cited by 21 publications

References 102 publications

Engineering Pseudomonas putida KT2440 for the production of isobutanol

Engineering Pseudomonas putida KT2440 for the production of isobutanol

Production and Polymerization of Biobased Acrylates and Analogs

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