Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration

Hwangbo, Hanjun; Lee, Hyeongjin; Jin, Eun‐Ju; Lee, JaeYoon; Jo, Yunju; Ryu, Dongryeol; Kim, GeunHyung

doi:10.1016/j.bioactmat.2021.06.031

Cited by 62 publications

(50 citation statements)

References 45 publications

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“…Therefore, because gelatin has relatively low yield stress on 37 °C, we have opted for alginate instead of the gelatin as the support medium to achieve the aforementioned static crevasse formation and broad stable processing conditions. 31,32 In the submerged printing process, the self-healing or elastic recoverable property of the supporting solution can be a significant factor. 28 To assess the elastic recovery of the alginate supporting solution, a strain sweep was conducted with different strain conditions (low strain = 10%, whereas high = 1000%) (Figure 2E).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Engineered Myoblast-Laden Collagen Filaments Fabricated Using a Submerged Bioprinting Process to Obtain Efficient Myogenic Activities

2021

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The skeletal muscle tissue comprises a hierarchical fibrous structure with fully aligned myofibers. To obtain a unique aligned engineering construct for regenerating muscle tissue, we adopted a submerged bioprinting process. Here, 3 wt % collagen and 6 wt % alginate solutions were used as a matrix cell-encapsulating bioink and supporting solution in the printing bath, respectively. By manipulating the processing parameters (various alginate weight fractions in the bath, nozzle moving speed, and hydrostatic pressure), cell-laden filaments (∼50 μm in diameter) were successfully fabricated. They presented a high degree of alignment of the fibrillated collagen and meaningful initial viability (∼90%) of the C2C12 myoblasts. In vitro cellular responses indicated that fully aligned F-actin filaments of myoblasts were developed, resulting in a high degree of alignment/formation of myotubes, compared to that in the controls (>100 μm diameter of cell-laden filaments). Furthermore, the expression levels of various myogenic genes (Myod1, Myh2, and Myog) were measured using a reverse transcription polymerase chain reaction on day 21 of the cell culture, and the results showed that the cell-laden filaments with a small diameter had considerably greater gene expression levels (2.2−8-fold) than those with a relatively large diameter. Thus, the printing process described herein can provide a new potential biofabricating platform to obtain cell-laden engineering constructs for various tissues.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Engineered Myoblast-Laden Collagen Filaments Fabricated Using a Submerged Bioprinting Process to Obtain Efficient Myogenic Activities

2021

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“…[166][167][168] Moreover, several studies have been conducted to examine the capacity of the HUA scaffolds seeded with the myoblasts for regeneration of volumetric muscle defects in vivo. [59,[165][166]168,189] Gilbert-Honick et al have developed a fibrin hydrogel microfiber bundle as HUA scaffolds seeded with C2C12 cells to assess its functionality to treat volumetric muscle loss in a murine model of removing ≈30-50% of the tibialis anterior muscle (Figure 2A). After 4 weeks treated with the cell-seeded scaffolds, the removed defects recovered significant volume to approximate the morphology of native uninjured muscle, with a high myofiber density but without fibrosis.…”

Section: Annulus Fibrosusmentioning

confidence: 99%

“…Moreover, several studies have been conducted to examine the capacity of the HUA scaffolds seeded with the myoblasts for regeneration of volumetric muscle defects in vivo. [ 59,165–166,168,189 ] Gilbert‐Honick et al. have developed a fibrin hydrogel microfiber bundle as HUA scaffolds seeded with C2C12 cells to assess its functionality to treat volumetric muscle loss in a murine model of removing ≈30–50% of the tibialis anterior muscle ( Figure A).…”

Section: Applications In Tissue Engineeringmentioning

confidence: 99%

Engineering Complex Anisotropic Scaffolds beyond Simply Uniaxial Alignment for Tissue Engineering

Xing

Liu

Xu³

et al. 2021

Adv Funct Materials

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Anisotropic microarchitectures arising from an aligned organization of threadlike extracellular matrix (ECM) components or cells are ubiquitous in the human body, such as skeletal muscle, corneal stroma, and meniscus, for executing tissue-specific physiological functions. It is widely recognized that tissue engineering, whereby growing the implanted or endogenous cells in anisotropic scaffolds with geometrical resemblance to the ECM of targeted tissues, represents a promising solution for the structural and functional restoration of these anisotropic tissues. However, remarkable challenges remain in recapitulating the anisotropic complexities of native tissues beyond simply uniaxial alignment. Through unremitting endeavors over the past decade, some innovative bioengineering approaches are developed to tackle these challenges. This review focuses on the recent progress in modular assembly and 3D printing techniques exploited to construct complex anisotropic scaffolds with a key highlight on their accessibility and features for different types of anisotropies, based on understanding the whole picture of anisotropies beyond simply uniaxial alignment in native tissues, which are geometrically divided into three categories. Finally, the applications of these complex scaffolds in anisotropic tissue engineering, either in vitro modeling or in vivo regeneration, are explored.

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“…Gene network analysis presenting the pattern of co-expression in each group was generated based on Spearman's correlation. The visualization of Gene Network was conducted using RStudio (RStudio Desktop v1.4.1717 with R v4.1.1), as previously described [24].…”

Section: Bioinformatic Analysismentioning

confidence: 99%

Topical Application of Galgeunhwanggeumhwangryeon-Tang Recovers Skin-Lipid Barrier and Ameliorates Inflammation via Filaggrin-Thymic Stromal Lymphopoietin-Interleukin 4 Pathway

Ahn

Shin

et al. 2021

Medicina

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Background and objectives: The purpose of this study was to confirm the effect of Galgeunhwanggeumhwangryeon-tang (GGRT) on the skin barrier integrity and inflammation in an atopic dermatitis-like animal model. Materials and Methods: The model was established using lipid barrier elimination (LBE) in BALB/c mice. Ceramide 3B, a control drug, and GGRT were applied to the skin of LBE mice. Gross observation and histological examination were combined with measurement of skin score, trans-epidermal water loss, and pH. The expression of filaggrin, kallikrein-related peptidase 7 (KLK7), protease-activated receptor-2 (PAR-2), thymic stromal lymphopoietin (TSLP), and interleukin 4 (IL-4) was examined. Results: The effect of GGRT on atopic dermatitis was estimated in silico using two individual gene sets of human atopic dermatitis. In animal experiments, GGRT treatment reduced atopic dermatitis-like symptoms, as confirmed via gross and histological observations, skin score, pH change, and trans-epidermal water loss. The expression level of filaggrin increased in the skin of GGRT-treated mice compared to that in the LBE group. The expression levels of KLK7, PAR2, TSLP, and IL-4 were decreased in GGRT-treated mice skin compared to those in LBE mice. Conclusions: We demonstrated that GGRT restored the skin barrier and reduced inflammatory reactions in a murine model of atopic dermatitis.

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Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration

Cited by 62 publications

References 45 publications

Engineered Myoblast-Laden Collagen Filaments Fabricated Using a Submerged Bioprinting Process to Obtain Efficient Myogenic Activities

Engineered Myoblast-Laden Collagen Filaments Fabricated Using a Submerged Bioprinting Process to Obtain Efficient Myogenic Activities

Engineering Complex Anisotropic Scaffolds beyond Simply Uniaxial Alignment for Tissue Engineering

Topical Application of Galgeunhwanggeumhwangryeon-Tang Recovers Skin-Lipid Barrier and Ameliorates Inflammation via Filaggrin-Thymic Stromal Lymphopoietin-Interleukin 4 Pathway

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