Bio‐positive effects of ionizing radiation on pollen: The role of ROS

Abstract

The concept of ‘hormesis’ is defined as a dose–response relationship whereby low doses of various toxic substances or physical stressors trigger bio‐positive effects in diverse biological systems, whereas high doses cause inhibition of cellular performance (e.g. growth, viability). The two‐sided phenomenon of specific low‐dose stimulation and high‐dose inhibition imposed by a ‘hormetic‐factor’ has been well documented in toxicology and pharmacology. Multitudinous factors have been identified that correspondingly cause hormetic effects in diverse taxa of animals, fungi, and plants. This study particularly aims to elucidate the molecular basis for stimulatory implications of ionizing radiation (IR) on plant male gametophytes (pollen). Beyond that, this analysis impacts general research on cell growth, plant breeding, radiation protection, and, in a wider sense, medical treatment. For this purpose, IR‐related data were surveyed and discussed in connection with the present knowledge about pollen physiology. It is concluded that IR‐induced reactive oxygen species (ROS) have a key role here. Moreover, it is hypothesized that IR‐exposure shifts the ratio between diverse types of ROS in the cell. The interrelation between ROS, intracellular Ca2+‐gradient, NADPH oxidases, ROS‐scavengers, actin dynamics, and cell wall properties are most probably involved in IR‐hormesis of pollen germination and tube growth. Modulation of gene expression, phytohormone signalling, and cellular antioxidant capacity are also implicated in IR‐hormesis.