Bio‐interfaces—Interaction of PLL/HA Thick Films with Nanoparticles and Microcapsules

Abstract

The interaction of biocompatible, exponentially grown films composed of poly-L-lysine (PLL) and hyaluronic acid (HA) polymers with gold nanoparticles and microcapsules is studied. Both aggregated and non-aggregated nanoparticle states are achieved; desorption of PLL accounts for aggregation of nanoparticles. The presence of aggregates of gold nanoparticles on films enables remote activation by near-infrared irradiation due to local, nanometer confined heating. Thermally shrunk microcapsules, which are remarkably monodisperse upon preparation but gain polydispersity after months of storage, are also adsorbed onto films. PLL polymers desorbed from films interact with microcapsules introducing a charge imbalance which leads to an increase of the microcapsule size, thus films amplify this effect. Multifunctional, biocompatible, thick gel films with remote activation and release capabilities are targeted for cell cultures in biology and tissue engineering in medicine.

Polyelectrolytes: Influence on Evaporative Self-Assembly of Particles and Assembly of Multilayers with Polymers, Nanoparticles and Carbon Nanotubes

“…Adsorption of MWCNTs on polyelectrolyte multilayer can be unambiguously concluded from these data. We note that broad absorption of carbon nanotubes in the visible spectral range [61][62][63][64] and their high light-to-heat conversion efficiency [67] makes them an attractive alternative to non-spherical nanoparticles [94] and nanorods [95,96] in regard to controlling the permeability of polymeric microcapsules [37][38][39][40][41][42][43] and activation of planar films [21][22][23]. We note that results of our work can be used for producing polyelectrolyte multilayers simultaneously functionalized not only with gold and magnetic nanoparticles [97], but also with these nanoparticles and carbon nanotubes.…”

“…during the build-up. First and foremost, planar films [18][19][20][21][22][23], as well as spherical particles and capsules [24][25][26][27][28][29][30], benefit from the multifunctionality that incorporation of diverse materials brings.…”

“…Demonstration of remote release inside living cells [43] enabled tracing intracellular processes relevant to immunology [44]. Besides, aggregates of nanoparticles embedded within biocompatible films can be activated by a near-IR laser [21][22][23]; these effects are based on nanoplasmonic heating of nanoparticles [45][46][47][48][49][50][51][52][53][54][55][56][57]. Additionally, magnetic nanoparticles enable positioning of polyelectrolyte capsules [58], while on the other hand activation is also possible [59].…”

Bio-interfaces: At the Junction of Material Science and Biology

“…A very big advantage of such films is their reservoir-like capacity to enclose bio-molecules as well as controllable diffusion of biomolecules in such films. Modification of such films with silver nanoparticles for SERS detection has been recently shown [6], while we have recently demonstrated functionalization of such films by gold nanoparticles [7]. Such a modification allows for selective surface modification of these films by remote laser irradiation [7], which can also serve the purpose of controlling mechanical properties of such films.…”

“…Modification of such films with silver nanoparticles for SERS detection has been recently shown [6], while we have recently demonstrated functionalization of such films by gold nanoparticles [7]. Such a modification allows for selective surface modification of these films by remote laser irradiation [7], which can also serve the purpose of controlling mechanical properties of such films. Mechanical properties, which are investigate by the so-called colloidal probe approach [8], are very important parameter of substrates and coatings since they alone can differentiate growth and proliferation of cells and tissue cells [9].…”