Bio-inspired silica–collagen materials: applications and perspectives in the medical field

Heinemann, Sascha; Coradin, Thibaud; Desimone, Martín Federico

doi:10.1039/c3bm00014a

Cited by 87 publications

(65 citation statements)

References 167 publications

(175 reference statements)

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“…As far as we know, the naturally occurring exposure concentration of silicon ions keep changed dynamically in different tissues or organs. A body of evidences indicates that serum Si concentration is ~0.1-0.2 μg/ml for most healthy adults 9,[46][47][48][49] , is peaked at ~0.8-1.2 μg/ml after intake of silicon-containing food 9, 46, 47 , and even yields 15-fold higher for hemodialysis patients up to ~3.3 μg/ml (their basal level is 0.9 μg/ml) when they take in silicon food 50 . In the current work, the plain medium used has similar silicon concentration in human physiological serum.…”

Section: Discussionmentioning

confidence: 99%

“…Si is an important element for mammals. For example, reduced amounts of collagen were found in silicon-deficient chicks and dietary silicon intake was good for bone mineral density in men and premenopausal women 9 . A serial of calcium-silicate based bioceramics, including bioglass, calcium silicate, dicalcium silicate, tricalcium silicate, akermanite, and others is known to affect the behaviors of stem cells in the past two decades.…”

Section: Introductionmentioning

confidence: 99%

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Bioactive calcium silicate extracts regulate the morphology and stemness of human embryonic stem cells at the initial stage

Zhang

Lü

et al. 2016

RSC Adv.

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Embryonic stem cells (ESCs) are undifferentiated cells that have the capacity to self-renew and differentiate into a variety of cells and provide cell sources for regenerative medicine or biological specimens for cytotoxicity tests. Calcium silicate (CS), a bioactive silicate ceramic, can stimulate the osteogenic differentiation of various types of stem cells, but its role in regulating the biological phenotypes of ESCs remains unclear. Here, the impact of CS on human ESCs was investigated using CS-supplemented medium. The cytotoxicity of CS to hESCs and its effects on apoptosis, growth, proliferation, and differentiation were quantified systematically. Morphological analysis of hESC colonies indicated that the bioactive ions released from CS have little cytotoxicity to hESCs at two CS concentrations. Immunofluorescence and flow cytometry analyses showed that apoptosis was time-independent at early or late stages of hESC growth. In contrast, CS ion extracts regulated hESC differentiation in a time-dependent manner: ESC stemness was preserved by enhancing Oct-4, Sox-2, and Nanog gene expression at day 3, while the cells tended to differentiate at day 6. Combined tests on gene and protein levels further indicated that hESCs tended to differentiate into mesoderm in the presence of CS ion extracts, especially at low CS concentrations. These results demonstrate the effects of CS extracts on hESC stemness and differentiation at the molecular and cellular levels, suggesting that CS-based biomaterials could serve as a potential regulator for ESCs in regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioactive calcium silicate extracts regulate the morphology and stemness of human embryonic stem cells at the initial stage

Zhang

Lü

et al. 2016

RSC Adv.

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“…Usually, approaches related to the in vitro silicification of organic templates under biomimetic conditions in vitro are carried out at temperatures between 20 and 37°C and at near-neutral pH (for review see [25]). However, nature exhibits several examples indicating that biological silicification also takes place under very extreme conditions, as reported in the introduction.…”

Section: Silicification Of Chitinous Scaffoldsmentioning

confidence: 99%

Preparation of monolithic silica–chitin composite under extreme biomimetic conditions

Bazhenov¹,

Wysokowski²,

Petrenko³

et al. 2015

International Journal of Biological Macromolecules

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“…The potentialities of silica nanoparticles for biological applications have now been well-identified, including molecular and gene delivery [1,2], small animals and cell imaging [3], photodynamic therapy [4], biomaterials surface functionalization [5] and hydrogels reinforcement [6,7]. Currently, strategies for optimizing drug loading, stealthiness, cell targeting as well as controlled release properties are being developed at a fast rate [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Evaluation of Median Lethal Concentrations of Stöber Silica Particles with Various Sizes and Surface Functionalities Towards Fibroblast Cells

Gonzalez

Álvarez

Camporotondi

et al. 2014

Silicon

Self Cite

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International audienceThe application of silica particles in the biomedical field has experienced a great development in recent years, especially in the design of nanoparticles having homogeneous size, structure and amenable to specific grafting. In this way, it becomes possible to control the interaction of nanoparticles with cells in order to meet the requirements for desired applications. This work explores the cytotoxicity of silica particles of various sizes and surface functionality towards L929 fibroblast cells. In particular, the median lethal concentration of the different silica particles has been established. Preliminary investigations of silica nanoparticles prepared by the Stöber method with sizes ranging from 100 nm to 500 nm showed that the largest particles are less harmful for the cells. Moreover, cytotoxicity towards L929 fibroblasts was mainly observed for bare particles, whereas sulfonate-, amine- and thiol-grafted particles had less detrimental effects. This shows the key influence of particle surface curvature and chemistry on nanomaterials cytotoxicity

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Bio-inspired silica–collagen materials: applications and perspectives in the medical field

Cited by 87 publications

References 167 publications

Bioactive calcium silicate extracts regulate the morphology and stemness of human embryonic stem cells at the initial stage

Bioactive calcium silicate extracts regulate the morphology and stemness of human embryonic stem cells at the initial stage

Preparation of monolithic silica–chitin composite under extreme biomimetic conditions

Preliminary Evaluation of Median Lethal Concentrations of Stöber Silica Particles with Various Sizes and Surface Functionalities Towards Fibroblast Cells

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