Bio-inspired metal ions regulate the structure evolution of self-assembled peptide-based nanoparticles

Xu, Anping; Yang, Peipei; Yang, Chao; Gao, Yujuan; Zhao, Xiaoxiao; Luo, Qiang; Li, Xiang‐Dan; Li, Lizhong; Wang, Lei; Wang, Hao

doi:10.1039/c6nr03580a

Cited by 50 publications

(39 citation statements)

References 41 publications

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“…The BKR initially self‐assembled into nanoparticles due to hydrophobic interactions, which further transformed into H bond stabilized nanofibers in situ induced by coordination with Ca 2+ . More importantly, the BKR nanoparticles could bind to U87 cell membrane through the interaction of RGD with Ca 2+ in α v β 3 integrins that result in morphology of BKR assemblies transformed from nanoparticles into nanofibers on U87 cell surfaces, leading to the cell death . Consequently, the same group constructed transformable NPs which could in situ transform into NFs and inhibit tumor invasion and metastasis in vivo.…”

Section: In Situ Constructed Peptide‐based Nanomaterialsmentioning

confidence: 84%

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Self‐Assembled Peptide‐Based Nanomaterials for Biomedical Imaging and Therapy

et al. 2018

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Peptide-based materials are one of the most important biomaterials, with diverse structures and functionalities. Over the past few decades, a self-assembly strategy is introduced to construct peptide-based nanomaterials, which can form well-controlled superstructures with high stability and multivalent effect. More recently, peptide-based functional biomaterials are widely utilized in clinical applications. However, there is no comprehensive review article that summarizes this growing area, from fundamental research to clinic translation. In this review, the recent progress of peptide-based materials, from molecular building block peptides and self-assembly driving forces, to biomedical and clinical applications is systematically summarized. Ex situ and in situ constructed nanomaterials based on functional peptides are presented. The advantages of intelligent in situ construction of peptide-based nanomaterials in vivo are emphasized, including construction strategy, nanostructure modulation, and biomedical effects. This review highlights the importance of self-assembled peptide nanostructures for nanomedicine and can facilitate further knowledge and understanding of these nanosystems toward clinical translation.

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Section: In Situ Constructed Peptide‐based Nanomaterialsmentioning

confidence: 84%

“…Schematic representation of metal ion binding induced reconstruction of BKR nanoassemblies from nanoparticles to nanofibers in solution and on the cell surface. Reproduced with permission . Copyright 2016, Royal Society of Chemistry.…”

Section: In Situ Constructed Peptide‐based Nanomaterialsmentioning

confidence: 99%

Self‐Assembled Peptide‐Based Nanomaterials for Biomedical Imaging and Therapy

et al. 2018

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“…Two absorption peaks centered at 265 and 480 nm appeared (Figure 1 A), representing the peptide backbone and DOX molecule, respectively, and the decrease in absorption over time revealed the formation of assembled aggregates. In particular, the absorbance at 265 nm decreased in a time-dependent manner, suggesting that DPC molecules aggregated through π-π interactions between aromatic residues 27 , 30 .…”

Section: Resultsmentioning

confidence: 99%

“…NIRF imaging was performed using a PE IVIS Spectrum imaging system for each group of mice at selected postinjection time points i.e. 0.5, 1, 2, 4, 6, 8, 10, 12, 18, 24, 36, 48, 60, 72 and 96 h. Three optical channels were recorded with selected excitation and emission bandpass filters 27 : Cy5.5 (λ ex =640 nm and λ em =720 nm); Cy7 (λ ex =745 and λ em = 800 nm); and FRET (λ ex =640 nm and λ em = 800 nm). The exposure time for each image was 0.1s.…”

Section: Methodsmentioning

confidence: 99%

Morphological transformation enhances Tumor Retention by Regulating the Self-assembly of Doxorubicin-peptide Conjugates

et al. 2020

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Rationale: Both spatial accuracy and temporal persistence are crucial in drug delivery, especially for anti-tumor intravenous nanomedicines, which have limited persistence due to their small particle sizes and easy removal from tumors. The present study takes advantage of morphological transformation strategy to regulate intravenous nanomedicines to display different sizes in different areas, achieving high efficient enrichment and long retention in lesions. Methods: We designed and synthesized functional doxorubicin-peptide conjugate nanoparticles (FDPC-NPs) consisting of self-assembled doxorubicin-peptide conjugates (DPCs) and an acidic-responsive shielding layer named the functional polylysine graft (FPG), which can regulate the assembly morphology of the DPCs from spherical DPC nanoparticles (DPC-NPs) to DPC-nanofibers (DPC-NFs) by preventing the assembly force from π-π stacking and hydrogen bond between the DPC-NPs. The morphology transformation and particle changes of FDPC-NPs in different environments were determined with DLS, TEM and SEM. We used FRET to explore the enhanced retention effect of FDPC-NPs in tumor site in vivo . HPLC-MS/MS analytical method was established to analyze the biodistribution of FDPC-NPs in H22 hepatoma xenograft mouse model. Finally, the antitumor effect and safety of FDPC-NPs was evaluated. Results: The FDPC-NPs were stable in blood circulation and responsively self-assembled into DPC-NFs when the FDPC-NPs underwent the acid-sensitive separation of the shielding layer in a mildly acidic microenvironment. The FDPC-NPs maintained a uniform spherical size of 80 nm and exhibited good morphological stability in neutral aqueous solution (pH 7.4) but aggregated into a long necklace-like chain structure or a crosslinked fiber structure over time in a weakly acidic solution (pH 6.5). These acidity-triggered transformable FDPC-NPs prolonged the accumulation in tumor tissue for more than 5 days after a single injection and improved the relative uptake rate of doxorubicin in tumors 31-fold. As a result, FDPC-NPs exhibited a preferable anti-tumor efficacy and a reduced side effect in vivo compared with free DOX solution and DOX liposomes. Conclusions: Morphology-transformable FDPC-NPs represent a promising therapeutic approach for prolonging the residence time of drugs at the target site to reduce side effect and enhance therapeutic efficacy. Our studies provide a new and simple idea for the design of long-term delivery systems for intravenous chemotherapeutic drugs.

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“…In addition, the self‐assembly of the short peptides to nanofibers could be mediated by metal ion such as Ca 2+ if the peptides are coupled with RGD . RGD is reported to coordinate with metal ions like Ca 2+ and Mg 2+ and this coordination was confirmed to stabilize the hydrogen bond between the peptide molecules and is the driving force to induce fiber formation.…”

Section: Hydrogels and Nanofibersmentioning

confidence: 98%

Molecular Self‐Assembly Constructed in Physiological Conditions for Cancer Diagnosis and Therapy

Qiao

Wang

2018

Advanced Therapeutics

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For the purpose of cancer theranostics, the performance and potential biosafety of drug/imaging agents remain major challenges. Although passive and active targeting strategies have been reported for effectively enhanced bioimaging or drug delivery performance, researchers are still looking for emerging methods for better targeting, accumulation, and penetration of solid tumors. The strategy for constructing supramolecular self-assemblies in biologically relevant conditions may partially overcome these challenges. Regarding the biomedical applications, supramolecular entities with naturally dynamic and modular properties, are capable of constructing structurally and functionally diversified materials and meeting the requirements of biological complexity. Modules and integrated systems enable response to bio-environment by the modification of their constitution through component exchange or reorganization. In this review, the authors summarize the self-assembled nanosystems (nanoparticles, nanoaggregates, micelles, vesicles, nanofibers, hydrogels, etc.) in physiological/pathological environments and their applications in cancer theranostics. The authors focus on chemical structures, stimuli-responsive bonds, and self-assembly property of nanomaterials, which may offer a guide for the optimal design of self-assembled nanomaterials in clinical cancer treatment and imaging.

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Bio-inspired metal ions regulate the structure evolution of self-assembled peptide-based nanoparticles

Cited by 50 publications

References 41 publications

Self‐Assembled Peptide‐Based Nanomaterials for Biomedical Imaging and Therapy

Self‐Assembled Peptide‐Based Nanomaterials for Biomedical Imaging and Therapy

Morphological transformation enhances Tumor Retention by Regulating the Self-assembly of Doxorubicin-peptide Conjugates

Molecular Self‐Assembly Constructed in Physiological Conditions for Cancer Diagnosis and Therapy

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