Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering

Dhand, Chetna; Ong, Seow Theng; Dwivedi, Neeraj; Diaz, Silvia Marrero; Venugopal, Jayarama Reddy; Navaneethan, Balchandar; Fazil, Mobashar Hussain Urf Turabe; Liu, Shouping; Seitz, Vera; Wintermantel, E.; Beuerman, Roger W.; Ramakrishna, Seeram; Verma, Navin Kumar; Lakshminarayanan, Rajamani

doi:10.1016/j.biomaterials.2016.07.007

Cited by 172 publications

(103 citation statements)

References 52 publications

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“…Overall, in situ surface polymerization technique is an efficient strategy to increase mineralization via chemical interactions between bioactive molecules and polymers during the polymerization process . Dhand et al exploited this technique to increase surface mineralization of substrate using CaCl 2 and collagen mats doped with catecholamines such as dopamine and norepinephrine . They observed that oxidative polymerization of catecholamines in the presence of divalent cations during electrospinning resulted in fabrication of collagen network with soldered junctions.…”

Section: Surface Modification Methodsmentioning

confidence: 99%

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Amani

Arzaghi

Bayandori

et al. 2019

Adv Materials Inter

305

200

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Surface interaction at the biomaterial–cell interface is essential for a variety of cellular functions, such as adhesion, proliferation, and differentiation. Nevertheless, changes in the biointerface enable to trigger specific cell signaling and result in different cellular responses. In order to manufacture biomaterials with higher functionality, biomaterials containing immobilized bioactive ligands have been widely introduced and employed for tissue engineering and regenerative medicine applications. Moreover, a number of physical and chemical strategies have been used to improve the functionality of biomaterials and specifically at the material interface. Here, the interactions between materials and cells at the interface levels are described. Then, the importance of surface properties in cell function is discussed and recent methods for surface modifications are systematically highlighted. Additionally, the impact of bulk material properties on the cellular responses is briefly reviewed.

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Section: Surface Modification Methodsmentioning

confidence: 99%

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Amani

Arzaghi

Bayandori

et al. 2019

Adv Materials Inter

305

200

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“…It is known that mechanical signals from materials have a profound impact on the lineage specification of MSCs [4–6]. The matrix stiffness is one of the most important physical attributes of the solid extracellular matrix (ECM) and directs the lineage specification of naive MSCs [7,8].…”

Section: Introductionmentioning

confidence: 99%

Mechanically cartilage-mimicking poly(PCL-PTHF urethane)/collagen nanofibers induce chondrogenesis by blocking NF–kappa B signaling pathway

et al. 2018

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Cartilage cannot self-repair and thus regeneration is a promising approach to its repair. Here we developed new electrospun nanofibers, made of poly (ε-caprolactone)/polytetrahydrofuran (PCL-PTHF urethane) and collagen I from calf skin (termed PC), to trigger the chondrogenic differentiation of mesenchymal stem cells (MSCs) and the cartilage regeneration in vivo. We found that the PC nanofibers had a modulus (4.3 Mpa) lower than the PCL-PTHF urethane nanofibers without collagen I from calf skin (termed P) (6.8 Mpa) although both values are within the range of the modulus of natural cartilage (1-10 MPa). Both P and PC nanofibers did not show obvious difference in the morphology and size. Surprisingly, in the absence of the additional chondrogenesis inducers, the softer PC nanofibers could induce the chondrogenic differentiation in vitro and cartilage regeneration in vivo more efficiently than the stiffer P nanofibers. Using mRNA-sequence analysis, we found that the PC nanofibers outperformed P nanofibers in inducing chondrogenesis by specifically blocking the NF-kappa B signaling pathway to suppress inflammation. Our work shows that the PC nanofibers can serve as building blocks of new scaffolds for cartilage regeneration and provides new insights on the effect of the mechanical properties of the nanofibers on the cartilage regeneration.

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“…2 However, the nanofibers themselves often lack intrinsic biofunctionalities, leading to poor cell adhesion, uncontrolled osteogenesis of progenitor cells, and eventually limitations in bone ingrowth. 3,4 Hence, many studies in this field have proposed the introduction of osteoinductive molecules such as osteogenic peptides or proteins on the surfaces of nanofibers through physical and chemical immobilization techniques. 5,6 For example, poly(lactic-co-glycolic acid) nanofibers immobilized with bone morphogenetic protein 2 enhanced in vivo bone regeneration by two times compared with that of unmodified fibers when they were transplanted into criticalsized femoral bone defects in rats.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Membrane Immobilized with Lactoferrin for Modulation of Bone Regeneration and Inflammation

Lee

et al. 2020

Tissue Engineering Part A

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Guided bone regeneration refers to the process in which bone defects could be regenerated by facilitated healing through the use of membranes, potentially with the delivery of osteoinductive molecules, however, the regeneration often failed due to inflammation during bone formation. In this study, we developed a membrane immobilized with lactoferrin to modulate both bone regeneration and inflammatory responses. Lactoferrin was immobilized on electrospun nanofibers (LF50) by exploiting an adhesive polydopamine coating method. When human adipose-derived stem cells (hADSCs) were seeded onto the nanofibers, the LF50 significantly increased the osteogenic differentiation. For example, the gene expression of osteopontin was 6.9 -2.3 times greater in the cells on LF50 than the cells on unmodified nanofibers without lactoferrin. In addition, the gene expression of tumor necrosis factor-alpha (TNF-a) of the macrophage cell line (RAW264.7) cultured on the LF50 was 0.3 -0.1 times reduced, indicating the lactoferrin was able to reduce inflammatory response. With implantation of nanofibers on in vivo mouse calvarial defects, the LF50 resulted in 60.9% -4.5% of new bone formation, which was six times greater than the results of other groups. Furthermore, when the fibers were implanted onto the in vivo mouse subcutaneous model challenged with lipopolysaccharide and interferon-g, the area of inflammatory tissue was significantly reduced in the LF50 implanted group as 0.6 -0.1 mm 2 as compared with the control group (1.1 -0.1 mm 2 ). Taken together, the lactoferrin immobilization onto the nanofiber by polydopamine chemistry may be an effective delivery method for improving bone regeneration while regulating the inflammation.In vivo critical-sized bone reconstruction remains challenging due to the severe inflammation, which would be an unavoidable problem during surgical process. Therefore, the present study aims to develop a guided nanofibrous membrane immobilized with lactoferrin, which has dual functions with osteoinduction and anti-inflammation. The lactoferrinimmobilized fibers demonstrated significantly enhanced in vitro osteogenic differentiation of adipose-derived stem cells as well as decreased polarization of macrophage to M1 with relatively reduced amount than that reported from previous reports. We also found that the membrane improved in vivo bone regeneration and decreased inflammatory tissue formation. Taken together, this system would be a new platform for successful bone regeneration.

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Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering

Cited by 172 publications

References 52 publications

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Mechanically cartilage-mimicking poly(PCL-PTHF urethane)/collagen nanofibers induce chondrogenesis by blocking NF–kappa B signaling pathway

Bioactive Membrane Immobilized with Lactoferrin for Modulation of Bone Regeneration and Inflammation

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