Bio-inspired green fabrication of silver nanoparticles (AgNPs) using aqueous leaves extract of Ipomoea carnea Jacq. To tackle multiple drug resistance MTCC bacterial strains

Singh, Ramveer; Sagar, Narashans Alok; Kumar, Nirbhay

doi:10.1016/j.ejmcr.2022.100066

Cited by 4 publications

(4 citation statements)

References 24 publications

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“…A lethality of 20% was identified at the highest concentration of 169.8 μg/mL with LC 50 value of 38062.7 μg/ml and cytotoxicity concentration corresponding to the published articles ( Kumar et al, 2012 ; Arulvasu et al, 2014 ). The findings of Yi Low S et al and Singh R. showed that LC 50 of AgNPs is recorded at higher concentrations ( Low et al, 2022 ; Singh et al, 2022 ). Whereas other research revealed that the cytotoxicity (LC 50 ) of AgNPs made using different approaches and reagents was seen at lower concentrations, i.e., 4 nM ( Arulvasu et al, 2014 ), 50 μg/mL ( Priyaragini et al, 2013 ), 518 μg/mL ( Asghar et al, 2020 ).…”

Section: Discussionmentioning

confidence: 93%

Molecular marker identification, antioxidant, antinociceptive, and anti-inflammatory responsiveness of malonic acid capped silver nanoparticle

Fatima,

Abrar,

Jahan

et al. 2024

Front. Pharmacol.

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Nano-sized silver has drawn a great deal of attention in the field of health sciences owing to its remarkable therapeutic applications. Interestingly, the method applied to synthesize nanoparticles and the choice of reagents considerably influence their therapeutic potential and toxicities. Current research has explored the toxicity, anti-inflammatory, antinociceptive, and antioxidant responses of the malonic acid-capped silver nanoparticles (MA-AgNPs (C) by using sodium borohydride as a reducing agent at low temperatures by employing both in vitro and in vivo approaches. Furthermore, it has highlighted the synergistic effect of these novel compounds with conventional anti-inflammatory therapeutic agents. Acute and sub-acute toxicity analysis performed following OECD guidelines showed that the studied MA-AgNPs (C) are safer, and prominent toxic signs have not been detected at the highest studied dose of 2,000 mg/kg. Cytotoxicity evaluation through brine shrimp lethality revealed 20% lethality at the highest concentration of 169.8 μg/mL. Significantly, positive anti-inflammatory and analgesic responses alone as well as synergism with the standard were identified through in vitro as well as in vivo methods which were more potent at a lower dose (200 mg/kg). Notably synergistic outcomes were more pronounced than individual ones, indicating their prominent effect as a feasible drug delivery system. IL-6 and TNF-α assessment in excised paw tissue through RTPCR technique further supported their anti-inflammatory potential. DPPH assay revealed eminent in vitro antioxidant activity which was further corroborated by in vivo antioxidant assessment through evaluation of SOD in excised paw tissue.

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Section: Discussionmentioning

confidence: 93%

Molecular marker identification, antioxidant, antinociceptive, and anti-inflammatory responsiveness of malonic acid capped silver nanoparticle

Fatima,

Abrar,

Jahan

et al. 2024

Front. Pharmacol.

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“…S. aureus wild-type strain MTCC 1144 was chosen for bacterial studies, which is a well-known clinical strain [26,27]. A single colony was picked from the overnight grown culture of S. aureus and inoculated into 5 ml of LB broth.…”

Section: Bacterial Studiesmentioning

confidence: 99%

“…Fenton reaction-mediated ROS generation and mechanical rupture by IONPs are responsible for its antibacterial activity [29]. The antibacterial test was conducted on the S. aureus MTCC 1144 strain, which is a clinical pathogen [26,27]. The bacterial strain was incubated with pristine and different OA-IONPs for 24 h, and the percentage survival of the bacteria with each treatment is shown in figure 5(a).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Influence of oleic acid coating on the magnetic susceptibility and Fenton reaction-mediated ROS generation by the iron oxide nanoparticles

Aadinath,

Muthuvijayan

2024

Nano Ex.

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Fenton reaction-mediated reactive oxygen species (ROS) generation by the iron oxide nanoparticles (IONPs) is responsible for its antibacterial activity. In general, IONPs are surface-coated to facilitate stability, control over size, biocompatibility, solubility, etc. We hypothesize that the extent of surface coating onto the IONPs might affect Fenton reaction-mediated ROS generation, which would eventually impact its antibacterial activity. In the present study, IONPs were prepared using the co-precipitation method, and different weights of oleic acid (OA) were loaded onto the IONPs. Pristine IONPs and oleic acid-coated iron oxide nanoparticles (OA-IONPs) were characterized using Fourier transform-infrared spectroscopy, dynamic light scattering, transmission electron microscopy, X-ray diffraction, vibrating sample magnetometry, goniometer, and thermogravimetric analysis. We found that magnetic susceptibilities of the IONPs were significantly enhanced with an increase in OA loading on the IONPs. The antibacterial study showed that the percentage inhibition was inversely related to the extent of oleic acid coating on the IONPs. The dependency of ROS generation on the extent of surface coating over IONPs was demonstrated using the 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) assay. Although pristine IONPs showed the least ROS generation, they exhibited maximum percentage inhibition of bacteria. This might be due to mechanical damage to the bacterial cells because of their crystalline nature. An in vitro biocompatibility study conducted on L929 fibroblast cell lines indicated that all the nanoparticle preparations were cytocompatible. This study concluded that the extent of surface coating influences the Fenton reaction-mediated ROS generation and also the magnetic susceptibilities of the IONPs.

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“…15,16 Among various metal nanoparticles, silver nanoparticles (AgNPs) have attracted much attention for applications in various fields, including biosensing materials, biomedicine, and wound dressings. [17][18][19] In addition, AgNPs are known as strong antibacterial agents due to their cytotoxic effects against various bacteria, the inhibition rate reached 100% at 125 ppm of AgNPs. 20 However, AgNPs aggregation in solution and duration of recovery are considered two critical challenges for the broad application of these nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Novel Bacterial Cellulose Based Silver-Metal Organic Frameworks (BC@Ag-MOF) as Antibacterial Wound Healing

Puspitasari,

Arief,

Ismadji

et al. 2023

Fine Chemical Engineering

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Bacterial Cellulose (BC) is a polymer derived from the bacterium Komagataeibacter xylinus with great potential for biomedical applications due to its high biocompatibility and biodegradability. In addition, the polymer is naturally biosynthesized by bacteria as hydrogels which can be used as optimal substrates for wound healing. However, the drawback of BC is the absence of antibacterial properties. Nowadays, some infections become more prevalent and harder to treat because of antimicrobial resistance, therefore, it is necessary to develop a strategy for modifying BC as wound healing that provides protection against bacterial contamination. In this work, silver-based Metal Organic Frameworks (MOFs) were immobilized into Bacterial Cellulose (BC). MOFs are porous coordination materials consisting of metal ions and multidentate organic ligands that have the potential as a matrix for metal ions due to their ability to gradually release metal ions. The structure and morphology of BC@Ag-MOF were successfully confirmed by Fourier-Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM). Evidently, BC@Ag-MOF exhibited a higher silver content (63.19%) than BC@Ag without immobilization into MOF (48.46%). Therefore, it indicated that MOF has large pores for enhancing the capacity for silver ion absorption in BC. The modified BC has never been reported and achieved the highest antibacterial activity of 99.99% against Gram-negative bacteria Escherichia coli. Moreover, BC@Ag-MOF has a higher antibacterial efficiency of 97% compared to BC@Ag without matrix. This study expands the potential application of BC modification in the field of biological antibacterial. has never been reported and achieved the highest antibacterial activity of 99.99% against Gram-negative bacteria Escherichia coli. Moreover, BC@Ag-MOF has a higher antibacterial efficiency of 97% compared to BC@Ag without matrix. This study expands the potential application of BC modification in the field of biological antibacterial.

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Bio-inspired green fabrication of silver nanoparticles (AgNPs) using aqueous leaves extract of Ipomoea carnea Jacq. To tackle multiple drug resistance MTCC bacterial strains

Cited by 4 publications

References 24 publications

Molecular marker identification, antioxidant, antinociceptive, and anti-inflammatory responsiveness of malonic acid capped silver nanoparticle

Molecular marker identification, antioxidant, antinociceptive, and anti-inflammatory responsiveness of malonic acid capped silver nanoparticle

Influence of oleic acid coating on the magnetic susceptibility and Fenton reaction-mediated ROS generation by the iron oxide nanoparticles

Synthesis of Novel Bacterial Cellulose Based Silver-Metal Organic Frameworks (BC@Ag-MOF) as Antibacterial Wound Healing

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