Bio‐Inspired Antioxidant Heparin‐Mimetic Peptide Hydrogel for Radiation‐Induced Skin Injury Repair

Abstract

Radiotherapy is one of the most important means of cancer treatment, however, radiation can also cause adverse reactions and even serious injuries to the skin. Radiation‐induced excess reactive oxygen species (ROS) production and inflammatory infiltration make skin wounds difficult to heal compared to normal skin injuries. Herein, an antioxidant heparin‐mimetic peptide hydrogel (K16, KYKYEYEYAGEGDSS‐4Sa) is designed for radiation‐induced skin injury (RISI) repair. First, the K16 peptide can self‐assemble into a hydrogel with a 3D mesh‐like porous nanofiber structure, which can provide certain physical support for skin repair like extracellular matrix (ECM). Then, K16 hydrogel not only scavenges ROS and prevents radiation damage to cellular DNA, but also promotes cell proliferation, migration, and angiogenesis. Meanwhile, 4‐sulfobenzoic acid (4Sa) modified at the N‐terminal end of the K16 peptide can adsorb inflammatory cytokines, thus acting to eliminate inflammation at the wound site. In vivo experiments showed that K16 hydrogel can inhibit early wound degradation, reduce inflammatory infiltration, and promote angiogenesis and collagen deposition, thus promoting wound healing. Therefore, the K16 hydrogel designed in this study has good potential for application in the field of radiation‐induced skin injury repair.

Hydrophobic Tetracycline Immobilized in Fibrous Hyaluronan Regulates Adhesive Collagen‐Based Hydrogel Stability for Infected Wound Healing

“…Cells Scratch Assay: The cell scratch assay was assessed by co-culture of hydrogel and cells, with some modifications based on the methods reported in the literature. [49] L929 cells were used in this study. Detailed methods are available in the Supporting Information.…”

Antioxidant Hydrogels: Antioxidant Mechanisms, Design Strategies, and Applications in the Treatment of Oxidative Stress‐Related Diseases

“…fabricated a 3D mesh‐like porous nanofiber structure hydrogel based on heparin‐mimicking polypeptide (AGEGDSS 4Sa) and self‐assembly peptide (KYKYEYEY). [ 46 ] The hydrogel was co‐synthesized by heparin's functional groups of AGEGDSS 4Sa with KYKYEYEY through a one‐step solid‐phase synthesis ( Figure a,b). The tyrosine within the KYKYEYEY structure could react with free radicals, thereby safeguarding cells against oxidative stress damage.…”

Extracellular Matrix Mimicking Wound Microenvironment Responsive Amyloid–Heparin@TAAgNP Co-Assembled Hydrogel: An Effective Conductive Antibacterial Wound Healing Material

“…Interestingly, earlier research results have established that heparin sulfate-based biomedical therapeutics can significantly reduce the LPS-induced inflammatory repercussions and act as a chemokine sequester. 48,49 Hence, our research was intended to investigate the potential of this impact in mitigating the production of ROS induced by LPS on macrophage cell lines. To do this, we have performed a cytocompatibility assessment for the LZ-HS(2:1)@TA AgNP20 assembly on cultured RAW264.7 cells.…”