Bio-inspired adhesive porous particles with human MSCs encapsulation for systemic lupus erythematosus treatment

Abstract

Mesenchymal stem cells (MSCs) therapy is a promising treatment for Systemic lupus erythematosus (SLE) patients. However, this method is encumbered by suboptimal phenotype of MSCs used in clinical settings, and a short in vivo persistence time. Herein, inspired by the natural microstructure of the sand tower worm nest, we proposed novel adhesive porous particles with human MSCs encapsulation via microfluidic electrospray technology for SLE treatment. The porous microparticles were formed by immediate gelation reaction between sodium alginate (ALG) and poly- d -lysine (PDL), and then sacrificed polyethylene oxide (PEO) to form the pores. The resultant microparticles could protect MSCs from immune cells while maintain their immune modulating functions, and achieve rapid exchange of nutrients from the body. In addition, owing to the electrostatic adsorption and covalent bonding between PDL and tissues, the porous microparticles could adhere to the bowel surfaces tightly after intraperitoneal injection. Through in vivo imaging system (IVIS) methods and in vivo study, it was demonstrated that the MSCs-encapsulated porous adhesive microparticles could significantly increase the cellular half-life, turn activated inflammatory macrophages into an anti-inflammatory profile, and ameliorate disease progression in MRL/ lpr mice. Thus, the MSCs-encapsulated porous microparticles showed distinctive functions in chronic SLE treatment, with additional potential to be used in a variety of biomedical applications.

Biomass Microcapsules with Stem Cell Encapsulation for Bone Repair

“…In addition, the diameter of the microcapsules began to decrease with the increasing voltage (Fig. S3c), which was consistent with previous studies [ 39 , 40 ]. The reason could be ascribed to that high dose of the biomass solution demonstrated a higher adhesion force between microcapsules, and a larger diameter of outer capillary led to larger droplets and therefore larger microcapsules [ 26 ].…”

“…In general, the microcapsules diameter was controlled by the common action of electric field force and liquid surface tension at the nozzle [ 39 , 40 ]. Taylor cone appears at the nozzle when the electric field force gradually increases.…”

How Microgels Can Improve the Impact of Organ-on-Chip and Microfluidic Devices for 3D Culture: Compartmentalization, Single Cell Encapsulation and Control on Cell Fate

“…Indeed, spatially confined cells can be precisely organized in space to achieve the desired 3D geometry, thus enabling the formation of either tunable or compartmentalized cell constructs. Microgels and their hydrophilic polymer networks can also confine cells while allowing the free diffusion of chemicals, nutrients, and metabolites and this is advantageous for cell therapy and modular tissue engineering, to guarantee immunoisolation, precise cell administration, and enhanced cell retention at the transplantation site [ 32 , 33 , 34 , 35 , 36 ]. When used in oil suspension, the confinement of cells in microgels facilitates the accumulation of cell secretions, enzyme molecules, and their catalytic products and that is attractive for the study of cellular functions, stimulus response, and cell-cell interactions [ 23 , 37 , 38 ].…”

Living Materials for Life Healthcare

“…Thus, the oxygen and nutrient transport was enhanced, and the cells demonstrated viability after 14 days of proliferation, as shown in Figure b. A similar method was later adopted for the encapsulation of human mesenchymal stem cells (MSCs) . The size of the pores was adjusted to protect the MSCs from immune cells while retaining their intrinsic functions.…”

“…Once injected, the NK cells could secret cytokines such as perforin, granzymes, and interferon-γ (IFN-γ), which would be sustainably released and trigger the apoptosis of tumor cells (Figure a). Similarly, a microsphere-based mesenchymal stem cell (MSC) therapy method has been implemented by encapsulating human MSCs for the treatment of systemic lupus erythematosus …”