Abstract:Drop-on-demand bioprinting allows the controlled placement of living cells, and will benefit research in the fields of tissue engineering, drug screening and toxicology. We show that a bio-ink based on a novel microgel suspension in a surfactant-containing tissue culture medium can be used to reproducibly print several different cell types, from two different commercially available drop-on-demand printing systems, over long printing periods. The bio-ink maintains a stable cell suspension, preventing the settli… Show more
“…Chahal et al successfully addressed this issue by using a surfactant (Ficoll-PM 400) to control the ink density resulting in reliable cell printing through a commercial single nozzle (MicroFab, Germany) over 90 min (Chahal et al 2012). Ferris et al showed that a bio-ink based on a novel microgel suspension in surfactant-containing tissue culture media could be used to prevent cell settling and aggregation (Ferris et al 2013). The stable suspension and optimal fluid properties of the bio-ink allowed reproducible printing of several different cell types, from two different commercially available drop-on-demand printing systems, over long printing periods.…”
Section: Parsa Et Al Used Single Piezoelectric Nozzle Ejectors (60-1mentioning
confidence: 99%
“…The issue of settling and aggregation of cells has recently been successfully addressed (Chahal et al 2012;Ferris et al 2013). Furthermore, work to date has utilised thermal inkjet , single-nozzle piezoelectric, and multiple nozzle piezoelectric printheads (Ferris et al 2013).…”
Section: Comparison Of Approaches and Remaining Challengesmentioning
confidence: 99%
“…Furthermore, work to date has utilised thermal inkjet , single-nozzle piezoelectric, and multiple nozzle piezoelectric printheads (Ferris et al 2013).…”
Section: Comparison Of Approaches and Remaining Challengesmentioning
The development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable and reproducible printing of cells. This review outlines the general principles and current progress and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs.
AbstractThe development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable, reproducible printing of cells. This review outlines the general principles, current progress, and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs.
“…Chahal et al successfully addressed this issue by using a surfactant (Ficoll-PM 400) to control the ink density resulting in reliable cell printing through a commercial single nozzle (MicroFab, Germany) over 90 min (Chahal et al 2012). Ferris et al showed that a bio-ink based on a novel microgel suspension in surfactant-containing tissue culture media could be used to prevent cell settling and aggregation (Ferris et al 2013). The stable suspension and optimal fluid properties of the bio-ink allowed reproducible printing of several different cell types, from two different commercially available drop-on-demand printing systems, over long printing periods.…”
Section: Parsa Et Al Used Single Piezoelectric Nozzle Ejectors (60-1mentioning
confidence: 99%
“…The issue of settling and aggregation of cells has recently been successfully addressed (Chahal et al 2012;Ferris et al 2013). Furthermore, work to date has utilised thermal inkjet , single-nozzle piezoelectric, and multiple nozzle piezoelectric printheads (Ferris et al 2013).…”
Section: Comparison Of Approaches and Remaining Challengesmentioning
confidence: 99%
“…Furthermore, work to date has utilised thermal inkjet , single-nozzle piezoelectric, and multiple nozzle piezoelectric printheads (Ferris et al 2013).…”
Section: Comparison Of Approaches and Remaining Challengesmentioning
The development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable and reproducible printing of cells. This review outlines the general principles and current progress and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs.
AbstractThe development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable, reproducible printing of cells. This review outlines the general principles, current progress, and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs.
“…pigmented inks), also generate another set of constraints (particle size, morphology and concentration) which are normally empirically evaluated for a given ink and print head combination. While a large number of different materials have been successfully printed by inkjet, including cells, colloids and nanomaterials (Ferris et al 2013, Perelaer et al 2006, van Deen et al 2013, ensuring that a material will print reliably and consistently in an industrial context is much more challenging and requires careful and long-term testing of the proposed system in conjunction with continued quality control.…”
Section: Section 1 Introduction To Inkjet Printing Technologiesmentioning
Abstract:Global regulatory, manufacturing and consumer trends are driving a need for change in current pharmaceutical sector business models, with a specific focus on the inherently expensive research costs, high-risk capital-intensive scale-up and the traditional centralised batch manufacturing paradigm. New technologies, such as inkjet printing, are being explored to radically transform pharmaceutical production processing and the end-to-end supply chain. This review provides a brief summary of inkjet printing technologies and their current applications in manufacturing before examining the business context driving the exploration of inkjet printing in the pharmaceutical sector. We then examine the trends reported in the literature for pharmaceutical printing, followed by the scientific considerations and challenges facing the adoption of this technology. We demonstrate that research activities are highly diverse, targeting a broad range of pharmaceutical types and printing systems. To mitigate this complexity we show that by categorising findings in terms of targeted business models and Active Pharmaceutical Ingredient (API) chemistry we have a more coherent approach to comparing research findings and can drive efficient translation of a chosen drug to inkjet manufacturing.
“…In the work by Ferris et al shown in Fig. 7a, neural (PC-12) and skeletal muscle (C2C12) cells were encapsulated within the low-acyl gellan gum by a droplet-based bioprinting technique [107]. After 8 days of culture, the construct exhibited the extension of dense neural networks from PC12 cells into surrounding areas populated by skeletal muscle cells.…”
Three-dimensional (3D) bioprinting is a computer-assisted technology which precisely controls spatial position of biomaterials, growth factors and living cells, offering unprecedented possibility to bridge the gap between structurally mimic tissue constructs and functional tissues or organoids. We briefly focus on diverse bioinks used in the recent progresses of biofabrication and 3D bioprinting of various tissue architectures including blood vessel, bone, cartilage, skin, heart, liver and nerve systems. This paper provides readers a guideline with the conjunction between bioinks and the targeted tissue or organ types in structuration and final functionalization of these tissue analogues. The challenges and perspectives in 3D bioprinting field are also illustrated.
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