Bio-informatics and in Vitro Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells

Abstract

Schisandrin A (SchA) has been reported to have good anti-cancer effects. However, its anticancer mechanism in breast cancer remains unknown. This study aimed to explore the mechanism of SchA in breast cancer treatment using bio-informatics analysis and in vitro experiments. The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Cards, and PharmMapper databases were used to screen the candidate targets of SchA against MDA-MB-231 cells selected as the tested cell line through MTT analysis. The functions and pathways of the targets were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and further analyzed using DAVID 6.8.1 database. Network pharmacology analysis revealed 77 candidate targets, 31 signal pathways, and 208 GO entries (P < 0.05). The targets regulated serine-type endopeptidase and protein tyrosine kinase activities, thereby promoting the migration and inhibiting the apoptosis of MDA-MB-231 cells. Comprehensive analysis of the "Protein-Protein Interaction" (PPI) and "Component-Targets-Pathways" (C-T-P) networks constructed using Cytoscape 3.7.1 software revealed four core targets: EGFR, PIK3R1, MMP9 and Caspase 3. Their docking scores with SchA were subsequently investigated through molecular docking. The wound healing, Hoechst 33342/PI, and western blot assays confirmed that SchA significantly down-regulated EGFR, PIK3R1, and MMP9, but up-regulated cleaved-caspase 3, thus inhibiting the migration and promoting the apoptosis of MDA-MB-231 cells. Reckoning the findings of the study, SchA is a potential adjuvant treatment for breast cancer.

In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

“…For example, although no relationship between serine-type endopeptidase activity and cervical cancer has been reported, it has been shown to be involved in and affecting many cancers, such as pancreatic adenocarcinoma, 45 ovarian cancer, 46 and breast cancer. 47 Collectively, the identified glycoproteins are significant in biological processes and molecular functions related to cervical cancer growth and may be directly or indirectly linked to cancer evolution and advancement. The above results indicate that Fe 3 O 4 @COF@MOF-PS is certainly excellent at isolating and enriching N -glycopeptides from complex samples and has the potential for screening possible disease markers of cervical cancer.…”

Transcriptomic analysis and experiments revealed that remimazolam promotes proliferation and G1/S transition in HCT8 cells

“…We also used combined EdU and PI staining to verify that remimazolam promoted the proliferation and cell-cycle progression of HCT8 cells. Integrated bioinformatics analysis is a significant approach for identifying main genes and pathways involved in disease pathogenesis (21). Our analysis revealed a total of 1,096 DEGs following remimazolam treatment, of which 673 genes were downregulated and 423 genes were upregulated.…”

“…Integrated bioinformatics analysis is a significant approach for identifying main genes and pathways involved in disease pathogenesis ( 21 ). Our analysis revealed a total of 1,096 DEGs following remimazolam treatment, of which 673 genes were downregulated and 423 genes were upregulated.…”

Uncovering the action mechanism of homoharringtonine against colorectal cancer by using network pharmacology and experimental evaluation

“…Network pharmacology is a promising approach to establishing ‘drug-target-disease’ network models by integrating system biology, bioinformatics and high-throughput screening technology [ 18 ]. Network pharmacology has been widely used for the mechanism research of natural products in different human diseases, including cancer [ 19 , 20 ]. In this study, we aimed to comprehensively elucidate the potential targets, biological processes, and molecular pathways of HHT to treat CRC by using the network pharmacology method.…”