Bio-Hydrogen Production using a Two-Stage Fermentation Process

Alalayah, Walid M.; Kalil, Mohd Sahaid; Kadhum, Abdul Amir H.; Jahim, Jamaliah Md; Jaapar, Syaripah Za`imah Sye; Alauj, Najeeb M.

doi:10.3923/pjbs.2009.1462.1467

Cited by 23 publications

(2 citation statements)

References 25 publications

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“…Clostridia are known as classical acid producers and usually ferment glucose to butyrate, acetate, carbon dioxide and molecular hydrogen (Alalayah et al, 2009b). Several statistical-design approaches used to optimise the hydrogen yield in fermentation processes have been reviewed (Wang and Wan, 2009).…”

Section: Reformation (Steam Reforming) Of Hydrogen-richmentioning

confidence: 99%

Applications of the Box-Wilson Design Model for Bio-hydrogen Production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Alalayah

Kalil²,

Kadhum³

et al. 2010

Pakistan J. of Biological Sciences

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Box-Wilson Design (BWD) model was applied to determine the optimum values of influencing parameters in anaerobic fermentation to produce hydrogen using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The main focus of the study was to find the optimal relationship between the hydrogen yield and three variables including initial substrate concentration, initial medium pH and reaction temperature. Microbial growth kinetic parameters for hydrogen production under anaerobic conditions were determined using the Monod model with incorporation of a substrate inhibition term. The values of µ (maximum specific max growth rate) and K (saturation constant) were 0.398 hG and 5.509 g LG , respectively, using glucose as the substrate concentration, 37°C reaction temperature and 6.0±0.2 initial medium pH gave 80% of the predicted maximum yield of hydrogen where as the experimental yield obtained in this study was 77.75% exhibiting a close accuracy between estimated and experimental values. This is the first report to predict bio-hydrogen yield by applying Box-Wilson Design in anaerobic fermentation while optimizing the effects of environmental factors prevailing there by investigating the effects of environmental factors.

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Section: Reformation (Steam Reforming) Of Hydrogen-richmentioning

confidence: 99%

Applications of the Box-Wilson Design Model for Bio-hydrogen Production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Alalayah

Kalil²,

Kadhum³

et al. 2010

Pakistan J. of Biological Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Unlike in dark fermentative H 2 production, that is, H 2 fermentation via hydrogenase by using nonphotosynthetic bacteria or archaea (5,6), PNSB can utilize short-chain organic acids as the substrate for H 2 production (1). Hence, various combinations of dark fermentation and photofermentation, for example, cocultures or two-stage processes, have been studied to develop a high-yield biohydrogen production system to secure a steady supply of H 2 as a renewable fuel alternative to fossil fuels (7)(8)(9)(10). As acetate is a fermentation product by many organisms from a variety of substrates, it is also a major by-product of dark fermentation (11).…”

mentioning

confidence: 99%

Introduction of Glyoxylate Bypass Increases Hydrogen Gas Yield from Acetate and l -Glutamate in Rhodobacter sphaeroides

Shimizu

Teramoto

Inui

2019

Appl Environ Microbiol

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Rhodobacter sphaeroides produces hydrogen gas (H2) from organic compounds via nitrogenase under anaerobic-light conditions in the presence of poor nitrogen sources, such as l-glutamate. R. sphaeroides utilizes the ethylmalonyl-coenzyme A (EMC) pathway for acetate assimilation, but its H2 yield from acetate in the presence of l-glutamate has been reported to be low. In this study, the deletion of ccr encoding crotonyl-coenzyme A (crotonyl-CoA) carboxylase/reductase, a key enzyme for the EMC pathway in R. sphaeroides, revealed that the EMC pathway is essential for H2 production from acetate and l-glutamate but not for growth and acetate consumption in the presence of l-glutamate. We introduced a plasmid expressing aceBA from Rhodobacter capsulatus encoding two key enzymes for the glyoxylate bypass into R. sphaeroides, which resulted in a 64% increase in H2 production. However, compared with the wild-type strain expressing heterologous aceBA genes, the strain with aceBA introduced in the genetic background of an EMC pathway-disrupted mutant showed a lower H2 yield. These results indicate that a combination of the endogenous EMC pathway and a heterologously expressed glyoxylate bypass is beneficial for H2 production. In addition, introduction of the glyoxylate bypass into a polyhydroxybutyrate (PHB) biosynthesis-disrupted mutant resulted in a delay in growth along with H2 production, although its H2 yield was comparable to that of the wild-type strain expressing heterologous aceBA genes. These results suggest that PHB production is important for fitness to the culture during H2 production from acetate and l-glutamate when both acetate-assimilating pathways are present. IMPORTANCE As an alternative to fossil fuel, H2 is a promising renewable energy source. Although photofermentative H2 production from acetate is key to developing an efficient process of biohydrogen production from biomass-derived sugars, H2 yields from acetate and l-glutamate by R. sphaeroides have been reported to be low. In this study, we observed that in addition to the endogenous EMC pathway, heterologous expression of the glyoxylate bypass in R. sphaeroides markedly increased H2 yields from acetate and l-glutamate. Therefore, this study provides a novel strategy for improving H2 yields from acetate in the presence of l-glutamate and contributes to a clear understanding of acetate metabolism in R. sphaeroides during photofermentative H2 production.

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Enhancement of Biohydrogen Production by Two-Stage Systems: Dark and Photofermentation

Keskin

Hallenbeck

2012

Biomass Conversion

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Bio-Hydrogen Production using a Two-Stage Fermentation Process

Cited by 23 publications

References 25 publications

Applications of the Box-Wilson Design Model for Bio-hydrogen Production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Applications of the Box-Wilson Design Model for Bio-hydrogen Production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Introduction of Glyoxylate Bypass Increases Hydrogen Gas Yield from Acetate and l -Glutamate in Rhodobacter sphaeroides

Enhancement of Biohydrogen Production by Two-Stage Systems: Dark and Photofermentation

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