Bio-harvesting and pyrolysis of the microalgae Botryococcus braunii

An optimized method for the bio-harvesting of microalgae, Botryococcus braunii, using Aspergillus sp. in large-scale studies

“…They are also finite resources and not a sustainable energy source (non-renewable). Some microalgae such Botryococcus braunii can be a viable alternative source of fuel, such as biodiesel and bio-oil, which are highly degradable [ 2 ]. This is because they are non-food feedstocks and are therefore not affected by co-demand by human population for use as food.…”

“… Method validation As the harvested B. braunii was likely to be used for biofuel production, it was crucial to show that harvesting these strains with the fungus did not cause any significant alteration in microalgae composition. Bioenergy analyses of the microalgae biomass harvested by flocculation with fungus and that of the microalgae harvested by centrifugation were carried out using pyrolysis (analytical Py–GC–MS and preparative pyrolysis) and compared [ 2 ]. Fig.…”

Co-culture for lipid production: Advances and challenges

“…This innovative approach was successfully investigated successfully with Aspergillus fumigatus [30,89]. The ability of fungi to flocculate microalgal species was widely investigated for wastewater treatment and Wrede et al [30] have conclusively demonstrated that bio-flocculation tends to enhance biomass and lipid production [30].…”

SingleChlamydomonas reinhardi(C. reinhardtii) cell separation from bacterial cells and auto-fluorescence tracking with a nano-sieve device

“…It has been shown that contaminations such as bacteria can cause nutrition competition and affect biomass productivity (Lian et al, 2018). Cell separation methods such as centrifugation, filtration, flocculation, ultrasonic acoustics, and fluorescence activated sorting are typically used to eliminate contaminations (Heaney et al, 1977; Al-Hothaly et al, 2015; Van den Hende et al, 2011; Bosma et al, 2003; Sensen et al, 1993). However, these traditional cell separation methods have several limitations such as cell damage, labor-intensive processing, high expense, difficulty in scaling, and contamination from reagents used in processing.…”