Bio-Functionalized Tapered Multimode Fiber Coated With Dengue Virus NS1 Glycoprotein for Label Free Detection of Anti-Dengue Virus NS1 IgG Antibody

Mustapa, M. A.; Bakar, M. H. Abu; Kamil, Yasmin Mustapha; Syahir, Amir; Mahdi, Mohd Adzir

doi:10.1109/jsen.2018.2813385

Cited by 14 publications

(12 citation statements)

References 29 publications

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“…It can be hypothesized that the lower limit of detection of this study is 0.00008 nM as any concentration less than 0.00008 nM is not detectable. The detection limit obtained was then compared with some of the recently published data as shown in Table 2 , which clearly shows that the Au/DSU/NH 2 rGO–PAMAM/IgM sensor film-based SPR sensor has the lowest detection limit so far [ 62 , 63 , 73 , 81 , 82 ]. Herein, the inclusion of DSU as a self-assembly monolayer and NH 2 rGO–PAMAM composite as a sensing layer has provided strong support for IgM immobilization for selective detection of dengue virus.…”

Section: Resultsmentioning

confidence: 85%

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

et al. 2021

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This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins.

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Section: Resultsmentioning

confidence: 85%

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

et al. 2021

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“…In the realm of photonics, several in-situ dengue diagnostics have been developed recently. A label free detection using multimode-tapered fiber, which is bio-functionalized with glycoprotein specifically reacts to the NS1 and IgG proteins under two minutes [9]. The use of surface plasmon resonance (SPR) method to examine the dynamics of IgM antigens has also gained traction recently [10].…”

Section: Rapid Detection Methods and Arising Issuesmentioning

confidence: 99%

Precursors to non-invasive clinical dengue screening: Multivariate signature analysis of in-vivo diffuse skin reflectance spectroscopy on febrile patients in Malaysia

et al. 2020

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Dengue diagnostics have come a long way. Attempts at breaking away from lab-oriented dengue detection, such as NS1 antigen, IgM or IgG antibodies detection have extensively received numerous coverage. As a result, rapid detection tests (RDTs) have started to gain inroads in medical practice. Rapid detection tests notwithstanding, analysis of blood serum is still a relatively complicated task. This includes the necessity of phlebotomy, centrifugation for blood serum, and other reagent-based tests. Therefore, a non-invasive method of dengue detection was considered. In this study, we present the utility of diffuse reflectance skin spectroscopy (bandwidth of 200-2500nm) on the forearm during the triaging period for dengue screening potential. This is performed with multivariate analysis of 240 triaged febrile/suspected dengue patients. The data is then scrutinized for its clinical validity to be included as either confirmed or probable dengue, or a control group. Based on discriminant analysis of several data normalization models, we can predict the patients' clinical denguepositivity at ranges of accuracy between~93-98% depending on mode of the data, with a probably optimal sensitivity and specificity to the clinical diagnosis of~89% and~100% respectively. From the outcomes of this study, we recommend further trials with cautious optimism. With these findings, it is hoped that the elusive non-invasive detection of tropical diseases may gain platform in the near future.

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“…This method has been widely used to detect different influenza A viruses, i.e., H1N1, H2N2, and H3N2 by labeling them with different colors. [ 35,36,56–58 ] The mass of individual viruses can be detected using silicon cantilever beams with a nanoscale thickness as shown in Figure 2e. The cantilever beam was fabricated with a dimension of 4–5 mm in length, 1–2 mm in width, and 20–30 nm in thickness.…”

Section: Flow‐free Optical Detectionmentioning

confidence: 99%

On‐Chip Optical Detection of Viruses: A Review

Shi

Liu

et al. 2021

Advanced Photonics Research

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The current outbreak of the coronavirus disease‐19 (COVID‐19) pandemic worldwide has caused millions of fatalities and imposed a severe impact on our daily lives. Thus, the global healthcare system urgently calls for rapid, affordable, and reliable detection toolkits. Although the gold‐standard nucleic acid amplification tests have been widely accepted and utilized, they are time‐consuming and labor‐intensive, which exceedingly hinder the mass detection in low‐income populations, especially in developing countries. Recently, due to the blooming development of photonics, various optical chips have been developed to detect single viruses with the advantages of fast, label‐free, affordable, and point of care deployment. Herein, optical approaches especially in three perspectives, e.g., flow‐free optical methods, optofluidics, and surface‐modification‐assisted approaches, are summarized. The future development of on‐chip optical‐detection methods in the wave of emerging new ideas in nanophotonics is also briefly discussed.

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Bio-Functionalized Tapered Multimode Fiber Coated With Dengue Virus NS1 Glycoprotein for Label Free Detection of Anti-Dengue Virus NS1 IgG Antibody

Cited by 14 publications

References 29 publications

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

Precursors to non-invasive clinical dengue screening: Multivariate signature analysis of in-vivo diffuse skin reflectance spectroscopy on febrile patients in Malaysia

On‐Chip Optical Detection of Viruses: A Review

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