Bio-functionalisation of polydimethylsiloxane with hyaluronic acid and hyaluronic acid – Collagen conjugate for neural interfacing

Yue, Zhilian; Liu, Xiao; Molino, Paul J.; Wallace, Gordon G.

doi:10.1016/j.biomaterials.2011.03.032

Cited by 58 publications

(59 citation statements)

References 44 publications

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“…PDMS is a suitable polymer due to its biocompatibility, 20,21 chemical stability, 22 modifiable stiffness, [23][24][25] and ability to be reused. PDMS micropillars can measure forces in the range of pico-Newtons to 100s of micro-Newtons.…”

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confidence: 99%

Colored polydimethylsiloxane micropillar arrays for high throughput measurements of forces applied by genetic model organisms

et al. 2015

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Measuring forces applied by multi-cellular organisms is valuable in investigating biomechanics of their locomotion. Several technologies have been developed to measure such forces, for example, strain gauges, micro-machined sensors, and calibrated cantilevers. We introduce an innovative combination of techniques as a high throughput screening tool to assess forces applied by multiple genetic model organisms. First, we fabricated colored Polydimethylsiloxane (PDMS) micropillars where the color enhances contrast making it easier to detect and track pillar displacement driven by the organism. Second, we developed a semi-automated graphical user interface to analyze the images for pillar displacement, thus reducing the analysis time for each animal to minutes. The addition of color reduced the Young's modulus of PDMS. Therefore, the dye-PDMS composite was characterized using Yeoh's hyperelastic model and the pillars were calibrated using a silicon based force sensor. We used our device to measure forces exerted by wild type and mutant Caenorhabditis elegans moving on an agarose surface. Wild type C. elegans exert an average force of ∼1 μN on an individual pillar and a total average force of ∼7.68 μN. We show that the middle of C. elegans exerts more force than its extremities. We find that C. elegans mutants with defective body wall muscles apply significantly lower force on individual pillars, while mutants defective in sensing externally applied mechanical forces still apply the same average force per pillar compared to wild type animals. Average forces applied per pillar are independent of the length, diameter, or cuticle stiffness of the animal. We also used the device to measure, for the first time, forces applied by Drosophila melanogaster larvae. Peristaltic waves occurred at 0.4 Hz applying an average force of ∼1.58 μN on a single pillar. Our colored microfluidic device along with its displacement tracking software allows us to measure forces applied by multiple model organisms that crawl or slither to travel through their environment.

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confidence: 99%

Colored polydimethylsiloxane micropillar arrays for high throughput measurements of forces applied by genetic model organisms

et al. 2015

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“…The amounts of surface accessible heparin were quantified using a TBO assay [29,30]. In general, AZ31 samples were immersed in a freshly prepared TBO solution (0.04 wt% in 0.01 M HCl/0.2 wt% NaCl solution, 2.0 ml per sample) and shaken gently at RT for 4 h followed by rinsing five times with MilliQ water.…”

Section: Physiochemical Characterisation Of the Modified Az31 Samplesmentioning

confidence: 99%

Biofunctionalized anti-corrosive silane coatings for magnesium alloys

et al. 2013

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Biodegradable magnesium alloys are advantageous in various implant applications, as they reduce the risks associated with permanent metallic implants. However, a rapid corrosion rate is usually a hindrance in biomedical applications. Here we report a facile two step procedure to introduce multifunctional, anticorrosive coatings on Mg alloys, such as AZ31. The first step involves treating the NaOH-activated Mg with bistriethoxysilylethane to immobilize a layer of densely crosslinked silane coating with good corrosion resistance; the second step is to impart amine functionality to the surface by treating the modified Mg with 3-amino-propyltrimethoxysilane. We characterized the two-layer anticorrosive coating of Mg alloy AZ31 by Fourier transform infrared spectroscopy, static contact angle measurement and optical profilometry, potentiodynamic polarization and AC impedance measurements. Furthermore, heparin was covalently conjugated onto the silane-treated AZ31 to render the coating haemocompatible, as demonstrated by reduced platelet adhesion on the heparinized surface. The method reported here is also applicable to the preparation of other types of biofunctional, anti-corrosive coatings and thus of significant interest in biodegradable implant applications.Keywords coatings, magnesium, alloys, corrosive, biofunctionalized, silane, anti Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsLiu, X., Yue, Z., Romeo, T., Weber, J., Scheuermann, T., Moulton, S. & Wallace, G. (2013). Biofunctionalized anti-corrosive silane coatings for magnesium alloys. Acta Biomaterialia, 9 (10), 8671-8677. AuthorsXiao Liu, Zhilian Yue, Tony Romeo, Jan Weber, Torsten Scheuermann, Simon E. Moulton, and Gordon G. Wallace AbstractBiodegradable magnesium (Mg) alloys are advantageous in various implant applications, as they reduce the risks associated with permanent metallic implants. However, the fast corrosion rate is usually a hindrance in biomedical applications. Here we report a facile twostep procedure to introduce multifunctional, anticorrosive coatings on Mg alloys, such as AZ31. The first step involves treating the NaOH-activated Mg with bistriethoxysilylethane (BTSE) to immobilise a layer of densely crosslinked silane coating with good corrosion resistance; the second step is to impart amine functionality to the surface by treating the modified Mg with 3-amino-propyltrimethoxysilane (γ-APS). We characterised the two-layer anticorrosive coating of AZ31 Mg alloy by Fourier transform infrared spectroscopy, static contact angle measurement and optical profilometry, potentiodynamic polarization and AC impedance measurements. Furthermore, heparin was covalently conjugated onto the silane treated AZ31 to render the coating haemocompatible, as demonstrated by reduced platelet adhesion on the heparinised surface. The method reported here is also applicable to the preparation of other types of biofunctional, anticorrosive coatings, and thus of significant interest in biodegradable implant applications.

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“…Our study [41], as well as others [27], has revealed that the environmental cue via cell adhesion molecules is essential for achieving optimal effect of neurotrophin on neurite extension. Indeed, controlling the presentation of cell adhesion molecules has been seen as an important strategy to improve neural-implant interfaces [80,81,82,83,84]. For instance, a synthetic protein bearing fibronectin fragments, SLPF, or a laminin fragment, CDPGYIGSR, was incorporated through the electrochemical deposition of PPy onto micro-machined silicon recording probes ( Fig.…”

Section: Ocp Coatings For Delivery Of Neuroactive Moleculesmentioning

confidence: 99%

Controlled delivery for neuro-bionic devices

Yue

Moulton

Cook

et al. 2013

Advanced Drug Delivery Reviews

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Implantable electrodes interface with the human body for a range of therapeutic as well as diagnostic applications. Here we provide an overview of controlled delivery strategies used in neuro-bionics. Controlled delivery of bioactive molecules has been used to minimise reactive cellular and tissue responses and/or promote nerve preservation and neurite outgrowth toward the implanted electrode. These effects are integral to establishing a chronically stable and effective electrode-neural communication. Drug-eluting bioactive coatings, organic conductive polymers, or integrated microfabricated drug delivery channels are strategies commonly used.

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Bio-functionalisation of polydimethylsiloxane with hyaluronic acid and hyaluronic acid – Collagen conjugate for neural interfacing

Cited by 58 publications

References 44 publications

Colored polydimethylsiloxane micropillar arrays for high throughput measurements of forces applied by genetic model organisms

Colored polydimethylsiloxane micropillar arrays for high throughput measurements of forces applied by genetic model organisms

Biofunctionalized anti-corrosive silane coatings for magnesium alloys

Controlled delivery for neuro-bionic devices

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