Bio‐functional hydrogel with antibacterial and anti‐inflammatory dual properties to combat with burn wound infection

Abstract

Burn infection delays wound healing and increases the burn patient mortality. Consequently, a new dressing with antibacterial and anti‐inflammatory dual properties is urgently required for wound healing. In this study, we propose a combination of methacrylate gelatin (GelMA) hydrogel system with silver nanoparticles embed in γ‐cyclodextrin metal–organic frameworks (Ag@MOF) and hyaluronic acid‐epigallocatechin gallate (HA‐E) for the burn wound infection treatment. Ag@MOF is used as an antibacterial agent and epigallocatechin gallate (EGCG) has exhibited biological properties of anti‐inflammation and antibacterial. The GelMA/HA‐E/Ag@MOF hydrogel enjoys suitable physical properties and sustained release of Ag + . Meanwhile, the hydrogel has excellent biocompatibility and could promote macrophage polarization from M1 to M2. In vivo wound healing evaluations further demonstrate that the GelMA/HA‐E/Ag@MOF hydrogel reduces the number of the bacterium efficiently, accelerates wound healing, promotes early angiogenesis, and regulates immune reaction. A further evaluation indicates that the noncanonical Wnt signal pathway is significantly activated in the GelMA/HA‐E/Ag@MOF hydrogel treated group. In conclusion, the GelMA/HA‐E/Ag@MOF hydrogel could serve as a promising multifunctional dressing for the burn wound healing.

Copper-Epigallocatechin Gallate Enhances Therapeutic Effects of 3D-Printed Dermal Scaffolds in Mitigating Diabetic Wound Scarring

“…The compressive modulus of 20% EM is the highest, which is 258 kPa, but it breaks when the deformation exceeds 40.6%. The compression modulus of 15% EM is 201 kPa, which can withstand 74% deformation and still maintain its shape, which is larger than the dermis of human skin …”

“…Swelling experiments were conducted according to the gravimetric method reported previously . Briefly, the hydrogel samples were weighed, and the initial weight was recorded as W 0 .…”

“…Swelling experiments were conducted according to the gravimetric method reported previously. 27 Briefly, the hydrogel samples were weighed, and the initial weight was recorded as W 0 . Subsequently, they were immersed in PBS solution at 37 °C and retrieved at certain time points (1, 2, 3, 4, 5, and 6 h).…”

“…The compression modulus of 15% EM is 201 kPa, which can withstand 74% deformation and still maintain its shape, which is larger than the dermis of human skin. 27 Referring to previous studies, the viscoelasticity of the material was used to characterize the stability of the hydrogels. The storage modulus (G′) of all concentrations of EM is significantly larger than the loss modulus (G″), which illustrates their solid elastic properties (Figure 2H).…”

Recent progress of antibacterial hydrogel materials for biomedical applications

“…For burn-infected wounds, wound healing can be accelerated by reducing bacterial numbers, regulating immune responses, and promoting early angiogenesis. Xiong et al 206 used a methacrylate gelatin (GelMA) hydrogel system with embedded g-cyclodextrin metal-organic frameworks (Ag@MOF) and hyaluronic acid-epigallocatechin gallate (HA-E) to prepare a novel dressing with antibacterial and anti-inflammatory properties for the treatment of infection in burn wounds. GelMA/HA-E/Ag@MOF hydrogels can effectively reduce bacterial population, accelerate wound healing, promote early angiogenesis, and modulate the immune response.…”