Bio-fabrication of silver nanoparticles by phycocyanin, characterization, in vitro anticancer activity against breast cancer cell line and in vivo cytotxicity

Abstract

In recent decades, researchers were attracted towards cyanobacterial components which are potential low-cost biological reagents for silver nanoparticle biosynthesis. This article describes the biological synthesis of silver nanoparticles using a proteinaceous pigment phycocyanin extracted from Nostoc linckia as reducing agent. The synthesized silver nanoparticles have a surface plasmon resonance band centered at 425 nm. Face-centered central composite design used for optimization of silver nanoparticles (AgNPs) biosynthesis using phycocyanin. The maximum AgNPs biosynthesis obtained using the optimized four variables, initial pH level (10), AgNO3 concentration (5 mM), phycocyanin pigment concentration (1 mg/mL) and incubation period (24 h) was 1100.025 µg/mL. The TEM analysis of AgNPs showed spherical nanoparticles with mean size between 9.39 to 25.89 nm. FTIR spectra showed major peaks of proteins involved in AgNPs biosynthesis by identifying different functional groups involved in effective capping of AgNPs. The biosynthesized AgNPs significantly inhibited the growth of medically important pathogenic Gram-positive (Staphylococcus aureus), Gram-negative bacteria (Pseudomonas aeruginosa, E. coli and Klebsiella pneumonia). The synthesized AgNPs exhibited effective cytotoxic activity against MCF-7 and the inhibitory concentration (IC50) was recorded at 27.79 ± 2.3 µg/mL. The in vivo studies clearly indicated that AgNPs has a capacity to inhibit the growth of tumor in Ehrlich ascites carcinoma bearing mice.

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

“…Biofunctional silver nanoparticles synthesized within various plant extracts of clove and guava evidenced the satisfying anti-cancer impact versus four different human cancer cell lines; colorectal adenocarcinoma, kidney, chronic myelogenous, leukemia, bone marrow, and cervix. 59 Moreover, Kishore et al 55 reported that apoptotic morphological changes were caused by AgNPs using DAPI staining method. 57…”

Silver nanoparticles: A novel antibacterial agent for control of Cronobacter sakazakii

“…In this study, the synthesized AgNP were spherical with a mean particle size of 31.2 nm, similar to the results of Ranoszek-Soliwoda et al (2017) but with a narrower size distribution due to the reducing capacity of both citrate and glycerol in boiling systems. The zeta potential was +18.7 mV, carrying the opposite charge to certain silver nanoparticles prepared by bio-fabrication, for example, using a proteinaceous pigment phycocyanin extracted from Nostoc linckia as the reducing reagent (El-Naggar et al, 2017). Meanwhile, the AgNP in the current study proved to be stable in aqueous solution with such zeta potential due to sufficient electrostatic repulsion.…”

“…This result showed the involvement in AgNP of nitrogen atoms, which might have originated from the dispersant PVP used in the preparation process. The bands at 1137.05, 1050.43, and 995.95 cm −1 were assigned to the stretching vibrations of primary amine C-N and C-C (Jyoti et al, 2016;El-Naggar et al, 2017). These bands were shifted to a lower wavenumber at 925.47 cm −1 due to the spatial resistance of AgNP.…”

“…Silver nanoparticles are metal nanoparticles with a size distribution from 1 to 100 nm in 3-dimensional space, and AgNP have generated significant interest as effective antimicrobial agents in recent years (El-Naggar et al, 2017;Chai et al, 2018). Silver nanoparticles exhibit strong antibacterial activity against Escherichia coli, Staphylococcus aureus, Vibrio cholerae, Pseudomonas aeruginosa, Eberthella typhi, and Bacillus subtilis (Morones et al, 2005;Ruparelia et al, 2008;Ramalingam et al, 2016;Gomaa, 2017).…”

“…The antibacterial property depends on the size distribution, crystal structure, and surface zeta potential of silver nanoparticles (Pal et al, 2007;Carlson et al, 2008). El-Naggar et al (2017) hypothesized that Ag nanoparticles cause cell death due to a decrease in cellular ATP production and DNA damage.…”