Bio-fabrication of peptide-modified alginate scaffolds: Printability, mechanical stability and neurite outgrowth assessments

Sarker, Md; Naghieh, Saman; McInnes, Adam D.; Ning, Liqun; Schreyer, David J.; Chen, Daniel

doi:10.1016/j.bprint.2019.e00045

Cited by 55 publications

(50 citation statements)

References 29 publications

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“…In another study, the influence of ionic crosslinkers on printability was investigated without considering other factors [10]. In some studies, only printing parameters were investigated as critical factors influencing printability [2,11]. In another study, the gelation properties of materials during the printing process were studied to achieve a mechanically stable structure [12].…”

Section: Introductionmentioning

confidence: 99%

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

et al. 2019

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Extrusion-based bioprinting of hydrogel scaffolds is challenging due to printing-related issues, such as the lack of capability to precisely print or deposit hydrogels onto three-dimensional (3D) scaffolds as designed. Printability is an index to measure the difference between the designed and fabricated scaffold in the printing process, which, however, is still under-explored. While studies have been reported on printing hydrogel scaffolds from one or more hydrogels, there is limited knowledge on the printability of hydrogels and their printing processes. This paper presented our study on the printability of 3D printed hydrogel scaffolds, with a focus on identifying the influence of hydrogel composition and printing parameters/conditions on printability. Using the hydrogels synthesized from pure alginate or alginate with gelatin and methyl-cellulose, we examined their flow behavior and mechanical properties, as well as their influence on printability. To characterize the printability, we examined the pore size, strand diameter, and other dimensions of the printed scaffolds. We then evaluated the printability in terms of pore/strand/angular/printability and irregularity. Our results revealed that the printability could be affected by a number of factors and among them, the most important were those related to the hydrogel composition and printing parameters. This study also presented a framework to evaluate alginate hydrogel printability in a systematic manner, which can be adopted and used in the studies of other hydrogels for bioprinting.

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Section: Introductionmentioning

confidence: 99%

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

et al. 2019

Self Cite

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“…However, an increase in extracellular matrix stiffness can affect cell development and differentiation [37][38][39] . For example, stem cell differentiation either into neuron-like cells or into muscles is respectively modulated by stiffness values of the environment with low (0.1-1 kPa) and intermediate stiffness (8)(9)(10)(11)(12)(13)(14)(15)(16)(17) 40 . For buccal mucosa HGF development, an equilibrium development was reached at a stiffness of 2 kPa 41 .…”

Section: Resultsmentioning

confidence: 99%

Nucleotide lipid-based hydrogel as a new biomaterial ink for biofabrication

Dessane

Smirani

Bouguéon

et al. 2020

Sci Rep

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One of the greatest challenges in the field of biofabrication remains the discovery of suitable bioinks that satisfy physicochemical and biological requirements. Despite recent advances in tissue engineering and biofabrication, progress has been limited to the development of technologies using polymer-based materials. Here, we show that a nucleotide lipid-based hydrogel resulting from the self-assembly of nucleotide lipids can be used as a bioink for soft tissue reconstruction using injection or extrusion-based systems. To the best of our knowledge, the use of a low molecular weight hydrogel as an alternative to polymeric bioinks is a novel concept in biofabrication and 3D bioprinting. Rheological studies revealed that nucleotide lipid-based hydrogels exhibit suitable mechanical properties for biofabrication and 3D bioprinting, including i) fast gelation kinetics in a cell culture medium and ii) shear moduli and thixotropy compatible with extruded oral cell survival (human gingival fibroblasts and stem cells from the apical papilla). This polymer-free soft material is a promising candidate for a new bioink design.Biofabrication is a growing field in regenerative medicine and represents a promising tool for the construction of complex 3D structures 1-4 . Several approaches 5,6 allow 3D-bioprinted construction to be achieved, with three main techniques: inkjet, laser and extrusion-based bioprinting 7 . Extrusion-based bioprinting (EBB) is one of the most widespread tools used in biofabrication, mainly due to its capacity to build large-volume constructs such as tissue or organ equivalents 5,8 . This technique offers a wide range of opportunities because it is suitable for various polymeric materials including scaffold-based (hydrogels, microcarriers, decellularised matrix components) as well as scaffold-free (cell aggregates) materials 5 .Murphy et al. 1 presented their concept of the "ideal material", which needs to display several features, including printability, biocompatibility, good degradation kinetics, and favourable structural and mechanical properties, as well as material biomimicry. According to recent definitions 9 , a bioink can be defined as "a formulation of cells that is suitable to be processed by an automated biofabrication technology". The presence of cells is a key element, and formulations that do not contain cells or involve the seeding of cells after printing do not qualify as bioinks. The term "biomaterial ink" (BmI) is then used. Consequently, this distinction will be used throughout our work.Hydrogels are commonly used for the formulation of bioinks or BmIs and seem to be promising biomaterials because they are affordable, are easy to handle and can mimic the extracellular matrix 10 . Initial approaches of EBB used synthetic polymers such as polyethylene glycol (PEG) or gelatine methacrylate (GelMA) as scaffold materials, but they suffered from poor biological properties 11 . As an alternative, natural polymers derived from fibrous proteins or peptides have been developed due to their intr...

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“…In this example, the NGF gradient is intended to attract sensory axons, and the GDNF gradient increases Schwann cell migration within a specific pathway. Since both Schwann cells and DRG cells preferentially follow the RGD peptide and laminin pathways, coating these additives on the surface of a silicone scaffold could be useful to enhance and direct axonal growth . Nevertheless, transplantation of these scaffolds into a 10 mm complex nerve injury in rats demonstrated successfully guided nerve regeneration after 4–6 weeks in vivo, resulting in enhanced functional recovery of the regenerated nerve .…”

Section: Peripheral Nervous System Regenerationmentioning

confidence: 99%

3D Printed Neural Regeneration Devices

Joung

Lavoie

Guo

et al. 2019

Adv Funct Materials

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Neural regeneration devices interface with the nervous system and can provide flexibility in material choice, implantation without the need for additional surgeries, and the ability to serve as guides augmented with physical, biological (e.g., cellular), and biochemical functionalities. Given the complexity and challenges associated with neural regeneration, a 3D printing approach to the design and manufacturing of neural devices can provide next-generation opportunities for advanced neural regeneration via the production of anatomically accurate geometries, spatial distributions of cellular components, and incorporation of therapeutic biomolecules. A 3D printing-based approach offers compatibility with 3D scanning, computer modeling, choice of input material, and increasing control over hierarchical integration. Therefore, a 3D printed implantable platform can ultimately be used to prepare novel biomimetic scaffolds and model complex tissue architectures for clinical implants in order to treat neurological diseases and injuries. Further, the flexibility and specificity offered by 3D printed in vitro platforms have the potential to be a significant foundational breakthrough with broad research implications in cell signaling and drug screening for personalized healthcare. This progress report examines recent advances in 3D printing strategies for neural regeneration as well as insight into how these approaches can be improved in future studies.spinal cord, and the peripheral nervous system (PNS), composed of the cranial and spinal nerves along with their associated ganglia that connect the CNS to the body. These systems are interconnected via an extensive network of nerves and neural cells (i.e., neurons and supporting glial cells) to facilitate communication and relay information (sensorimotor signals) to and from all parts of the body.

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Bio-fabrication of peptide-modified alginate scaffolds: Printability, mechanical stability and neurite outgrowth assessments

Cited by 55 publications

References 29 publications

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

Nucleotide lipid-based hydrogel as a new biomaterial ink for biofabrication

3D Printed Neural Regeneration Devices

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