Bio-Encapsulation Within Sol-Gel Glasses

Bergogne, L.; Fennouh, Souad; Guyon, Stéphanie; Livage, Jacques; Roux, Cécile

doi:10.1080/10587250008023605

Cited by 13 publications

(9 citation statements)

References 20 publications

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“…Despite the undisputed importance and technical challenges involved, there are only a few reports on the encapsulation of live cells in sol−gel matrixes. − Carturan and co-workers reported the immobilization of Saccharomyces cerivisiae yeasts and Coronilla vaginalis plant cells in silicate matrixes . Carturan and co- workers further showed viability of rat hepatocytes in layered SiO 2 −collagen matrixes, and Pope and co-workers 8 encapsulated pancreatic cells in sol−gel silicates.…”

Section: Introductionmentioning

confidence: 99%

“…Carturan and co- workers further showed viability of rat hepatocytes in layered SiO 2 −collagen matrixes, and Pope and co-workers 8 encapsulated pancreatic cells in sol−gel silicates. Braun used silica−alginate encapsulation for studies of the biosynthetic pathway of itaconic acid in fungus Aspergillus terreus . , Livage and Roux studied the encapsulation of Leishmania parasites for recognition of antibodies in human blood . Zink, Dunn, and co-workers patterned silicate networks with bacterial cells …”

Section: Introductionmentioning

confidence: 99%

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Fluorescent Bacteria Encapsulated in Sol−Gel Derived Silicate Films

et al. 2002

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Recombinant fluorescent E. coli cells were encapsulated in sol-gel derived silicate films. Green and red fluorescent proteins expression by recombinant E. coli strains within the silicate film were studied. The effect of the preparation protocol on the viability of the encapsulated cells and their ability to express the fluorescent proteins in response to genotoxicity stress were evaluated. Confocal microscopy studies revealed a homogeneous distribution of the recombinant cells within the silicate film. We further showed by confocal microscopy studies that the encapsulated cells do not divide within the silicate film. Further, dynamic green fluorescent protein expression studies of a single encapsulated cell were carried out for the first time. No contamination of the surroundings by the encapsulated cells or contamination of the encapsulated culture by the surrounding population of bacteria was observed. The implications of these observations for dual sensing and multistep biosynthesis and biodegradation are discussed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fluorescent Bacteria Encapsulated in Sol−Gel Derived Silicate Films

et al. 2002

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“…Some bioencapsulation studies have quantified the containment and outgrowth of encapsulated cells (Campostrini et al 1996Dire´et al 1992, Farid et al 1994, Inama et al 1993. However, typical sol-gel synthetic methods involving alkoxide precursors resulted in microporous gels (average pore size <2 nm), which restricted diffusion of nutrients, resulting in poor growth parameters (Bergogne et al 2000, Coiffier et al 2001. Furthermore, gels formed by conventional alkoxide routes release alcohols, which are toxic to the encapsulated cells.…”

Section: Introductionmentioning

confidence: 99%

“…Cell-immobilisation and encapsulation techniques using porous solid phases have been widely investigated for microbial systems (Bergogne et al 2000, Coiffier et al 2001, Dire´1992, Finnie et al 2000, Karube et al 1984. Some bioencapsulation studies have quantified the containment and outgrowth of encapsulated cells (Campostrini et al 1996Dire´et al 1992, Farid et al 1994, Inama et al 1993.…”

Section: Introductionmentioning

confidence: 99%

Quantification of the contribution of surface outgrowth to biocatalysis in sol-gels: oxytetracycline production by Streptomyces rimosus

Taylor

Finnie

Bartlett

et al. 2004

Biotechnology Letters

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A technique was developed for differentiating the activity of microbes solely within sol gels by using the contribution of biomass outgrowth. Streptomyces rimosus was immobilised in colloidal silica gels and biomass growth, oxytetracycline synthesis, pH and carbohydrate consumption were compared for UV surface-sterilised gels, untreated gels, and liquid cultures. Absolute and biomass specific oxytetracycline yields were higher for non-sterile gels than for liquid culture. Biomass solely within colloidal silica gels (1.7 mg ml(-1)), and gels obtained from colloidal silica modified by addition of larger silica particles (1.2 mg ml(-1)) yielded 27 and 21 microg ml(-1) oxytetracycline compared with 97 and 104 microg ml(-1) for unsterilised gels (3.6 and 5.2 mg ml(-1) biomass) displaying outgrowth. It was therefore apparent that biomass and antibiotic production within the gels was limited and that optimisation requires gel modification.

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“…Depending on experimental conditions, sol−gel protocols enable encapsulation of biomolecules retaining their conformation and catalytic activity. Even viable cells such as yeasts − or bacteria − can be embedded and still maintain their viability (“living composites, biocers”) . The reliable immobilization of biocomponents is crucial for application of biocers in remediation technologies.…”

Section: Introductionmentioning

confidence: 99%

Biosorption of Uranium and Copper by Biocers

et al. 2002

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Biological ceramic composites (biocers) made according to aqueous sol-gel protocol were used as selective metal binding filters. The biological component of the biocers Bacillus sphaericus JG-A12 was isolated from a uranium mining waste pile. Vegetative cells and spores of this strain are known to bind selectively U, Cu, Al, Cd, and Pb in large amounts. Sol-gel ceramics were prepared by dispersing vegetative cells, spores, and stabilized surfacelayer proteins (S-layer) in aqueous silica nanosols, gelling, and drying. The biosorption of uranium and copper by the three kinds of biocers and by their single components was investigated with dependence on time, concentration, and preparation conditions. Biocers with cells possess the highest binding capacity compared to matrixes with spores and an S-layer. Freeze-drying of prepared biocers or adding water-soluble compounds as sorbitol lead to higher porosity and faster metal binding. Uranium was bound mainly to the biological component but also to the SiO 2 network. In contrast, copper was only bound by the cells, spores, or S-layer. Bound uranium and copper were completely removed by washing with aqueous citric acid.

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Bio-Encapsulation Within Sol-Gel Glasses

Cited by 13 publications

References 20 publications

Fluorescent Bacteria Encapsulated in Sol−Gel Derived Silicate Films

Fluorescent Bacteria Encapsulated in Sol−Gel Derived Silicate Films

Quantification of the contribution of surface outgrowth to biocatalysis in sol-gels: oxytetracycline production by Streptomyces rimosus

Biosorption of Uranium and Copper by Biocers

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