Bio-electrosprays and Aerodynamically Assisted Bio-jets, Flow Cytometry Concepts for Interrogating Living Cells and Whole Organisms

Abstract

Flow cytometry (1), a Florescent Activated Cell Sorting (FACScan) approach has truly revolutionised the identification and interrogation of cellular health through rapid single cell analysis (2). This unique cell assessment approach has not only capabilities for identifying the cell's health and other molecular components of cells, but has become a viable approach for sorting homogeneous populations of cells from heterogeneous cellular populations (3). Today flow cytometry enormously contributes to both research and clinical studies. In fact, post-flow cytometered cells have been explored in in vivo research and clinical studies (4, 5).Although, flow cytometry has achieved some notable milestones, one feature that has not been addressed by flow cytometry is the ability to apply this methodology for interrogating and sorting living whole organisms. In most embryo research, scientist sort embryos (for e.g. transgenic organisms) manually and thus, is time consuming