Bio-efficacy of insecticidal molecule emodin against dengue, filariasis, and malaria vectors

Ragavendran, Chinnasamy; Balasubramani, Govindasamy; Venkatesh, Manigandan; Magudeeswaran, Sivanandam; Arulbalachandran, D.; Willie, Peijnenburg; Vivekanandhan, Perumal; Mekchay, Supamit; Krutmuang, Patcharin

doi:10.1007/s11356-023-26290-0

Cited by 9 publications

(1 citation statement)

References 85 publications

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“…When various chemical insecticides, such as permethrin, cypermethrin, monocrotophos, and malathion, are repeatedly used, insects develop resistance to these chemical pesticides ( Metcalf, 1989 ; Gahukar and Kishore, 1995 ; Vivekanandhan et al, 2021 ). As a result, various methods of controlling insects using secondary metabolites from plants, bacteria, and fungi have shown greater efficacy ( Balumahendhiran et al, 2019 ; Logeswaran et al, 2019 ; Pratheeba et al, 2019 ; Vivekanandhan et al, 2022a , b ; Chinnasamy et al, 2023 ; Kannan et al, 2023 ; Krutmuang et al, 2023 ; Perumal et al, 2023a , b , c ).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol effects of chemical molecules derived from Beauveria bassiana against larvae of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Vivekanandhan,

Swathy,

Alahmadi

et al. 2024

Front. Microbiol.

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In this study, we conducted tests on the isolation, identification, characterization, and extraction of chemical molecules from Beauveria bassiana against Tuta absoluta larvae. The enzyme responses of T. absoluta to the crude extract were examined 24 h after treatment, and the number of dead larvae was calculated 24 and 48 h after treatment. Molecular docking studies were conducted to assess the interaction of important molecules with the acetylcholinesterase enzyme. The larvicidal activity of crude chemicals from fungi was high 24 h after treatment, with LC50 and LC90 values of 25.937 and 33.559 μg/mL, respectively. For a period of 48 h, the LC50 and LC90 values were 52.254 and 60.450 μg/mL, respectively. The levels of acetylcholinesterase, α-carboxylesterase, and β-carboxylesterase enzymes were lower in the treatment group after 24 h compared to the control group. The GC-MS test revealed that the crude extract consisted mainly of 9,10-octadecadienoic acid, which was the primary compound. Docking results indicated that 9,10-octadecadienoic acid showed a strong interaction with acetylcholinesterase (AChE). Our findings suggest that the chemical molecule 9,10-octadecadienoic acid derived from the entomopathogenic fungus B. bassiana is more toxic to T. absoluta larvae. We plan to conduct studies to test its effectiveness in semi-field conditions and to evaluate its stability in field conditions. We believe that this 9,10-octadecadienoic acid molecule could be used to control T. absoluta larvae in the near future without causing environmental pollution.

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Section: Introductionmentioning

confidence: 99%

Biocontrol effects of chemical molecules derived from Beauveria bassiana against larvae of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Vivekanandhan,

Swathy,

Alahmadi

et al. 2024

Front. Microbiol.

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Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae)

Vivekanandhan,

Alahmadi,

Ansari

2024

J Basic Microbiol

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Tenebrio molitor L., also known as the mealworm, is a polyphagous insect pest that infests various stored grains worldwide. Both the adult and larval stages can cause significant damage to stored grains. The present study focused on isolating entomopathogenic fungi from an infected larval cadaver under environmental conditions. Fungal pathogenicity was tested on T. molitor larvae and pupae for 12 days. Entomopathogenic fungi were identified using biotechnological methods based on their morphology and the sequence of their nuclear ribosomal internal transcribed spacer (ITS). The results of the insecticidal activity indicate that the virulence of fungi varies between the larval and pupal stages. In comparison to the larval stage, the pupal stage is highly susceptible to Metarhizium rileyi, exhibiting 100% mortality rates after 12 days (lethal concentration 50 [LC50] = 7.8 × 106 and lethal concentration 90 (LC90) = 2.1 × 1013 conidia/mL), whereas larvae showed 92% mortality rates at 12 days posttreatment (LC50 = 1.0 × 106 and LC90 = 3.0 × 109 conidia/mL). The enzymatic analyses revealed a significant increase in the levels of the insect enzymes superoxide dismutase (4.76–10.5 mg−1) and glutathione S‐transferase (0.46–6.53 mg−1) 3 days after exposure to M. rileyi conidia (1.5 × 105 conidia/mL) compared to the control group. The findings clearly show that M. rileyi is an environmentally friendly and effective microbial agent for controlling the larvae and pupae of T. molitor.

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Biocontrol efficacy of entomopathogenic fungi Beauveria bassiana conidia against agricultural insect pests

Swathy,

Parmar,

Vivekanandhan

2024

Environmental Quality Mgmt

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Insect pests are serious threats to agriculture, forestry, and human health because they damage crops and trees and spread diseases. Chemical insecticides control insect pests quickly and effectively, protecting crops. Environmental and health concerns arise from their use. Long‐term exposure can cause pesticide‐resistant insects, requiring stronger chemicals. Beneficial insects and wildlife may be harmed. Some chemical insecticides persist in the environment, causing long‐term ecological damage. The present study was to isolate, identify, and characterize entomopathogenic fungi from the soil, evaluate their pathogenicity against major insect species, and evaluate the non‐target effect on soil bioindicator species. Bioassay results show that Beauveria bassiana conidia are more pathogenic to all three species at 10 days after treatment, causing 100% mortality in Halyomorpha halys and Tenebrio molitor within 10 days. The lethal concentration showed lower LC50 values of 9.5 × 103 conidia/mL in H. halys, 2.6 × 103 conidia/mL in T. molitor, and 8.3×104 conidia/mL in P. japonica, B. bassiana treatment results showed a shortened insect life time LT50 of H. halys (6.0 days), T. molitor (5.3 days), and P. japonica (6.9 days). The present study concluded that B. bassiana fungi conidia are more efficient against three major insect pests.

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Bio-efficacy of insecticidal molecule emodin against dengue, filariasis, and malaria vectors

Cited by 9 publications

References 85 publications

Biocontrol effects of chemical molecules derived from Beauveria bassiana against larvae of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Biocontrol effects of chemical molecules derived from Beauveria bassiana against larvae of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae)

Biocontrol efficacy of entomopathogenic fungi Beauveria bassiana conidia against agricultural insect pests

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