Bio-efficacy of Different Strains of Bacillus spp. against  Meloidogyne incognita under in vitro

Mahesha, H. S.; Ravichandra, N. G.; Rao, M. S.; Narasegowda, N.C.; Sonyal, Shreeshail; Hotkar, Shivalingappa

doi:10.20546/ijcmas.2017.611.295

Cited by 8 publications

(9 citation statements)

References 2 publications

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“…Among nematodes, the viability of T. colubriformis eggs was reduced by 89.96% 1 h after treatment with 0.13 μg mL –1 Btk solution 32 . Eggs of M. incognita treated with various strains of Bt for 120 h resulted in a loss of viability of 33–76.67% 42 . The correlation between inviability and concentration of the entomopathogen used in this study is similar to that observed in P. xylostella eggs treated with Btk.…”

Section: Discussionsupporting

confidence: 79%

Bioactivity of Bacillus thuringiensis (Bacillales: Bacillaceae) on Diatraea saccharalis (Lepidoptera: Crambidae) eggs

Daquila

Dossi

Moi

et al. 2021

Pest Management Science

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BACKGROUND Bacillus thuringiensis (Bt) is a Gram‐positive bacterium that synthesizes specific protein toxins, which can be exploited for control of various insect pests, including Diatraea saccharalis, a lepidopteran that severely damages sugarcane crops. Although studies have described the effects of Bt in the larval phases of D. saccharalis, few have examined its effect on insect eggs. Herein, we studied the entomopathogenic potential of Bacillus thuringiensis serovar Aizawai GC‐91 (Bta) during D. saccharalis embryo development with the aim of understanding the entomopathogenic mechanism and developing new biological control techniques for target insects. RESULTS Bta concentrations of 5, 10 and 20 g L–1 demonstrated the strongest bioactivity, reducing D. saccharalis egg viability by 28.69%, 33.91% and 34.98%, respectively. The lethal concentrations (LCs) were estimated as: LC50 = 28.07 g L–1 (CI 95% = 1.89–2.38) and LC90 = 65.36 g L–1 (CI 95% = 4.19–5.26). Alterations in egg coloration, melanization and granule accumulation were observed at 24 h, persisting until 144 h. The embryo digestive systems were severely damaged, including narrowing of the intestinal lumen, vesiculations and degenerated cells, causing embryonic death. CONCLUSION The toxicity caused by Bta in D. saccharalis embryos demonstrated its potential as a biological control agent and as a sustainable alternative for integrated management of D. saccharalis infestation. © 2020 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 79%

Bioactivity of Bacillus thuringiensis (Bacillales: Bacillaceae) on Diatraea saccharalis (Lepidoptera: Crambidae) eggs

Daquila

Dossi

Moi

et al. 2021

Pest Management Science

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“…It was also noted that 90.0% of hatching got inhibited at 2 WAI by 10.0% concentration of both bacteria. In a similar experiment, Mahesha et al (2017) tested the bio-efficacy of different strains of Bacillus spp., including B . subtilis , against M .…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial inoculum was streaked on the solidified medium in the petri dish. The media was then incubated at 28 °C at for 24 h ( Mahesha et al, 2017 ). A loop full of bacterial colony was taken out of the petri dish and aseptically transferred to the nutrient broth in a 250 ml conical flask.…”

Section: Methodsmentioning

confidence: 99%

“…Conical flask was then incubated in an electric shaker for 48 h at 200 rpm at 32 °C ( Sela et al, 1998 ). After incubation, culture filtrates were harvested by centrifugation of the nutrient broth at 6000 rpm for 10 min ( Mahesha et al, 2017 ). Afterwards, the supernatant was passed through membrane filters having the pore size of 0.45 and 0.20 μm (CHROMAFIL® Xtra) subsequently and stored as stock solution in the refrigerator.…”

Section: Methodsmentioning

confidence: 99%

“…Data was recorded at every 1 week interval and continued until 6th week. Percent egg hatch inhibition over control was calculated using the formula ( Mahesha et al, 2017 ):

where C = Number of hatched J 2 in control and T = Number of hatched J 2 in treatment…”

Section: Methodsmentioning

confidence: 99%

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Functional evaluation of culture filtrates of Bacillus subtilis and Pseudomonas fluorescens on the mortality and hatching of Meloidogyne javanica

Das

Wadud

Khokon

2021

Saudi Journal of Biological Sciences

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Rhizospheric bacteria Bacillus subtilis and Pseudomonas fluorescens are two widely tested biological control agents against root-knot nematodes (RKN) of different crops. However, their performance as bio-control agents varies with their place of origin. Culture filtrates of rhizospheric bacteria contain some intermediary metabolites that have nematicidal activity. An in vitro experiment was undertaken to evaluate the functionality of culture filtrates of B. subtilis (MN252542.1) and P. fluorescens (MN256394.1) at different concentrations (1.0%, 2.5%, 5.0%, 7.0%, 10.0% and 25.0%) on the hatching and mortality of Meloidogyne javanica at different time span. Bacterial strains were isolated from rhizospheric soils of Bangladesh. At three days after incubation (DAI), 25.0% concentration of culture filtrates of both B. subtilis and P. fluorescens showed 100.0% mortality of second stage juveniles (J 2 ) of M. javanica . Additionally, 25.0% concentration of culture filtrates of both bacteria showed 100.0% inhibition of hatching at one week after incubation (WAI). A decreasing trend in hatching of M. javanica was observed with the increment of the concentration of culture filtrates and progression of incubation time. The findings of this experiment reveal that culture filtrates of these accessions of B. subtilis and P. fluorescens are effective for controlling M. javanica and would be potential candidates for developing bio-nematicides.

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Biorational management of root-knot of brinjal (Solanum melongena L.) caused by Meloidogyne javanica

Das

Wadud

Chakraborty

et al. 2022

Heliyon

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Bio-efficacy of Different Strains of Bacillus spp. against Meloidogyne incognita under in vitro

Cited by 8 publications

References 2 publications

Bioactivity of Bacillus thuringiensis (Bacillales: Bacillaceae) on Diatraea saccharalis (Lepidoptera: Crambidae) eggs

Bioactivity of Bacillus thuringiensis (Bacillales: Bacillaceae) on Diatraea saccharalis (Lepidoptera: Crambidae) eggs

Functional evaluation of culture filtrates of Bacillus subtilis and Pseudomonas fluorescens on the mortality and hatching of Meloidogyne javanica

Biorational management of root-knot of brinjal (Solanum melongena L.) caused by Meloidogyne javanica

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