Bio Efficacy of Atalantia monophylla (L) Correa (Rutaceae) against Spodoptera litura Fabricius (Lepidoptera: Noctuidae)

Abstract

Hexane, chloroform and ethyl acetate extracts of Atalantia monophylla were evaluated for their larvicidal and pupicidal activities against Spodoptera litura. Bioefficacy of A. monophylla leaf extracts were studied leaf disc no choice method at 0.5, 1.0, 2.5 and 5.0% concentration of crude extracts and 125, 250, 500 and 1000 ppm concentration of fractions against S. litura. The maximum larvicidal and pupicidal activities were noticed in hexane extract of A. monophylla. The hexane extract exhibited the least LC 50 value of 2.01% for larvicidal activity and regression value (R 0.98 and Coefficients 9.33 ± 17.1) for concentration dependent larvicidal activity. The hexane extract was fractionated using increasing polarity of solvent system. Twelve fractions were isolated and were evaluated at four different concentrations. Maximum antifeedant, larvicidal and pupicidal activities was noticed in fraction 9 at 1000 ppm concentration. Ninth fraction exhibited concentration dependent antifeedant and larvicidal activities with liner regression of R 0.93, and 0.93 respectively. Least LC 50 value of 340.27 ppm was observed in fraction nine for larvicidal activity. All the concentrations of 9 th fraction showed good activity.