Bio-conversion of apple pomace into ethanol and acetic acid: Enzymatic hydrolysis and fermentation

Parmar, Indu; Rupasinghe, H.P. Vasantha

doi:10.1016/j.biortech.2012.12.084

Cited by 91 publications

(40 citation statements)

References 21 publications

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“…Large quantities of waste are produced worldwide every year, with 25–35 % of the 70 million metric tonnes produced ending up as waste (Food and Agricultural Organisation of the United Nations 2012; Gullon et al 2008), an estimated 17–24 million metric tonnes per year (Van Dyk et al 2013). In most cases, the pomace is disposed of, which poses major environmental and health problems due to its high moisture content (70–75 %), high chemical oxygen demand (COD, 10,000 mg/L) and biological oxygen demand (BOD) (Bhushan et al 2008; Capek et al 1995; Mahmood et al 2010; Parmar and Rupasinghe 2013). Combining treatment of this waste with production of value-added products can solve disposal problems, while potentially creating additional revenue in the bio-economy.…”

Section: Introductionmentioning

confidence: 99%

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Optimisation of enzymatic hydrolysis of apple pomace for production of biofuel and biorefinery chemicals using commercial enzymes

2015

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Apple pomace, a waste product from the apple juice industry is a potential feedstock for biofuel and biorefinery chemical production. Optimisation of hydrolysis conditions for apple pomace hydrolysis using Viscozyme L and Celluclast 1.5L was investigated using 1 L reaction volumes. The effects of temperature, pH, β-glucosidase supplementation and substrate feeding regimes were determined. Hydrolysis at room temperature using an unbuffered system gave optimal performance. Reactors in batch mode resulted in a better performance (4.2 g/L glucose and 16.8 g/L reducing sugar, 75 % yield for both) than fed-batch (3.2 g/L glucose and 14.6 g/L reducing sugar, 65.5 and 73.1 % yield respectively) in 72 h. The addition of Novozyme 188 to the core mixture of Viscozyme L and Celluclast 1.5L resulted in the doubling of glucose released. The main products (yield %) released from apple pomace hydrolysis were galacturonic acid (78 %), glucose (75 %), arabinose (90 %) and galactose (87 %). These products are potential raw materials for biofuel and biorefinery chemical production.

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Section: Introductionmentioning

confidence: 99%

“…(Bhushan et al 2008; Foyle et al 2007; Joshi and Attri 2006; Parmar and Rupasinghe 2013; Vendruscolo and Ninow 2014). Enzymatic degradation of lignocellulosic substrates requires a number of enzymes, working in synergy.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of enzymatic hydrolysis of apple pomace for production of biofuel and biorefinery chemicals using commercial enzymes

2015

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“…The difference with the results obtained in this work may be related to different substrates and the range of parameters used. In the work by Parmar and Rupasinghe (2013), pH, temperature, time and enzyme dosage were shown to be significant for the release of sugars from apple pomace using a combination of Celluclast 1.5L, Pectinex 3XL and Novozyme 188. Though the same substrate was used, the enzymes used were not all the same and the range of values for the parameters used were also different.…”

Section: Interactive Effect Of Different Hydrolysis Parametersmentioning

confidence: 99%

“…Apple pomace, a waste product from the apple juice industry, is a potential lignocellulosic feedstock for the production of biofuels and biorefinery chemicals (Gama et al 2015). Apple pomace is composed mainly of cellulose, pectin and hemicellulose, which can be converted to sugar monomers and other value-added products using multiple enzymes working in synergy (Bhushan et al 2008;Gama et al 2015;Joshi and Attri 2006;Parmar and Rupasinghe 2013). Enzymes required for the degradation of the cellulose component include cellobiohydrolases, endo-glucanases and b-glucosidases (Teeri 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Pectin degradation requires enzymes such as methyl esterase, acetyl esterase, pectate lyase, pectin lyase, endo-polygalacturonase, exopolygalacturonase, arabinase and galactanase (Gama et al 2015;Voragen et al 2009), while degradation of hemicellulose requires enzymes like endo-xylanases, bxylosidases, xyloglucanases, as well as possible debranching enzymes. Commercial enzyme cocktails are available which act on lignocellulosics, with different cocktails optimised for different substrates (Gama et al 2015;Parmar and Rupasinghe 2013).…”

Section: Introductionmentioning

confidence: 99%

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Using an artificial neural network to predict the optimal conditions for enzymatic hydrolysis of apple pomace

et al. 2017

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The enzymatic degradation of lignocellulosic biomass such as apple pomace is a complex process influenced by a number of hydrolysis conditions. Predicting optimal conditions, including enzyme and substrate concentration, temperature and pH can improve conversion efficiency. In this study, the production of sugar monomers from apple pomace using commercial enzyme preparations, Celluclast 1.5L, Viscozyme L and Novozyme 188 was investigated. A limited number of experiments were carried out and then analysed using an artificial neural network (ANN) to model the enzymatic hydrolysis process. The ANN was used to simulate the enzymatic hydrolysis process for a range of input variables and the optimal conditions were successfully selected as was indicated by the R 2 value of 0.99 and a small MSE value. The inputs for the ANN were substrate loading, enzyme loading, temperature, initial pH and a combination of these parameters, while release profiles of glucose and reducing sugars were the outputs. Enzyme loadings of 0.5 and 0.2 mg/g substrate and a substrate loading of 30% were optimal for glucose and reducing sugar release from apple pomace, respectively, resulting in concentrations of 6.5 g/L glucose and 28.9 g/L reducing sugars. Apple pomace hydrolysis can be successfully carried out based on the predicted optimal conditions from the ANN.

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Bioethanol from Waste – Prospects and Challenges of Current and Emerging Technologies

Valta

Papadaskalopoulou

Dimarogona

2019

Byproducts From Agriculture and Fisheries

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Bio-conversion of apple pomace into ethanol and acetic acid: Enzymatic hydrolysis and fermentation

Cited by 91 publications

References 21 publications

Optimisation of enzymatic hydrolysis of apple pomace for production of biofuel and biorefinery chemicals using commercial enzymes

Optimisation of enzymatic hydrolysis of apple pomace for production of biofuel and biorefinery chemicals using commercial enzymes

Using an artificial neural network to predict the optimal conditions for enzymatic hydrolysis of apple pomace

Bioethanol from Waste – Prospects and Challenges of Current and Emerging Technologies

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