Bio-competitive exclusion: efficacy of non-aflatoxigenic Aspergillus section Flavi-L morphotypes in control of aflatoxigenic Aspergillus flavus in groundnuts (Arachis hypogaea L.)

Chofamba, Anyway

doi:10.1186/s43088-021-00129-4

Cited by 2 publications

(2 citation statements)

References 25 publications

(30 reference statements)

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“…Further studies are warranted to determine whether the L‐morphotypes isolated in this study can be used as biological agent against toxigenic Aspergillus species. In many countries, sclerotium‐forming species of A. flavus , particularly the L‐morphotype, are being tested for their inability to produce aflatoxin and their potential use as a biological agent in control of aflatoxigenic A. flavus (Camiletti et al, 2018; Chofamba, 2021; El‐Aziz et al, 2021; Zanon et al, 2018; Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Aflatoxin levels and Aspergillus species in maize in the Province of Isabela, Philippines

Romero

Cumagun

2023

Journal of Phytopathology

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The province of Isabela is the top maize producer in the Philippines. The intensive cultivation and the tropical climate in the region may favour fungal growth and aflatoxin contamination in maize grains. Thus, the study aimed to determine the occurrence of aflatoxin and mycotoxigenic Aspergillus species in maize varieties in this region. Samples were obtained in six municipalities from hybrid (Bt) maize (n = 101) and open‐pollinated varieties (n = 6) during the dry season (March and April) of 2019. Aflatoxin levels were quantified through enzyme‐linked immunosorbent assay, and Aspergillus species were identified through cultural and molecular methods. Aflatoxin was detected in 50.5% of maize samples; 49.5% of samples were less than the limit of detection (3 μg/kg), 16.8% with 3–20 μg/kg, 10.9% with 21–50 μg/kg and 22.8% above 50 μg/kg. Samples within the acceptable level were 66.3% for food (<20 μg/kg) and 77.2% for animal feed (<50 μg/kg), while 22.8% of samples were above the acceptable level of the Philippine National Standard for raw maize grains. More than 90% of Aspergillus species detected were A. flavus. Other species identified were A. tamarii and A. terreus. Despite the dry production season in the province with low relative humidity during harvesting, inadequate post‐harvest practices and the presence of A. flavus elevated the level of aflatoxin in sample grains. Additional work involving multi‐year surveys is needed to confirm the results and conclusions of this study.

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Section: Discussionmentioning

confidence: 99%

Aflatoxin levels and Aspergillus species in maize in the Province of Isabela, Philippines

Romero

Cumagun

2023

Journal of Phytopathology

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“…This was taken to strongly support our suggestion that sexual reproduction between compatible isolates, leading to recombinant offspring, was an occurrence in the field. Such a projection is not favorable for the aflatoxin biocontrol strategies which rely on the competitive advantage of large populations of atoxigenic A. flavus over toxigenic field isolates [19,[54][55][56].…”

Section: Discussionmentioning

confidence: 99%

Cocoa‐associated filamentous fungi for the biocontrol of aflatoxigenic Aspergillus flavus

et al. 2023

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Aflatoxin and other mycotoxin contamination are major threats to global food security and present an urgent need to secure the global food crop against spoilage by mycotoxigenic fungi. Cocoa material is noted for naturally low aflatoxin contamination. This study was designed to assess the potential for harnessing cocoa‐associated filamentous fungi for the biocontrol of aflatoxigenic Aspergillus flavus. The candidate fungi were isolated from fermented cocoa beans collected from four cocoa‐growing areas in Ghana. Molecular characterization included Internal Transcribed Spacer (ITS)‐sequencing for identification and polymer chain reaction (PCR) to determine mating type. Effects of the candidate isolates on growth and aflatoxin‐production by an aflatoxigenic A. flavus isolate (BANGA1) were assessed. Aflatoxin production was monitored by UV fluorescence and quantified by enzyme‐linked immunosorbent assay (ELISA). Thirty‐six filamentous fungi were cultured and identified as Aspergillus, Cladosporium, Lichtheimia, or Trichoderma spp. isolates. The isolates generally interacted negatively with BANGA1 growth and aflatoxin production. The Aspergillus niger and Aspergillus aculeatus biocontrol candidates showed the strongest colony antagonism (54%–94%) and reduction in aflatoxin production (12%–50%) on agar. In broth, the A. niger isolates reduced aflatoxin production by up to 97%. Metabolites from the A. niger isolates showed the strongest inhibition of growth by BANGA1 and inhibited aflatoxin production. Four of the candidate isolates belonged to the MAT1‐1 mating type and 12 identified as MAT1‐2. This may be indicative of the potential for genetic recombination events between fungi in the field, a finding which is particularly relevant to the risk posed by A. flavus biocontrol measures that rely on atoxigenic A. flavus strains.

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Bio-competitive exclusion: efficacy of non-aflatoxigenic Aspergillus section Flavi-L morphotypes in control of aflatoxigenic Aspergillus flavus in groundnuts (Arachis hypogaea L.)

Cited by 2 publications

References 25 publications

Aflatoxin levels and Aspergillus species in maize in the Province of Isabela, Philippines

Aflatoxin levels and Aspergillus species in maize in the Province of Isabela, Philippines

Cocoa‐associated filamentous fungi for the biocontrol of aflatoxigenic Aspergillus flavus

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