Bio-coloration of bacterial cellulose assisted by immobilized laccase

Abstract

In this work a process for the bio-coloration of bacterial cellulose (BC) membranes was developed. Laccase from Myceliophthora thermophila was immobilized onto BC membranes and retained up to 88% of residual activity after immobilization. Four compounds belonging to the flavonoids family were chosen to test the in situ polymerase activity of immobilized laccase. All the flavonoids were successfully polymerized by laccase giving rise to yellow, orange and dark brown oligomers which conferred color to the BC support. The optimal bio-coloration conditions were studied for two of the tested flavonoids, catechol and catechin, by varying the concentration and time of incubation. High color depth and resistance to washing were obtained for both compounds. The highly porous bacterial cellulose material demonstrated great performance as a bio-coloration support, in contrast to other materials cited in literature, like cotton or wool. The process developed is presented as an environmentally friendly alternative for bacterial cellulose bio-coloration and will contribute deeply for the development of new fashionable products within this material.

Effect of Additives on the in situ Laccase-Catalyzed Polymerization of Aniline Onto Bacterial Cellulose

“…Regarding specific applications, bacterial cellulose has been explored as a template for the in situ polymerization of a panoply of compounds from phenols to polyamines. An example has been reported by Song et al who studied the in situ polymerization of several flavonoids by laccase onto a bacterial cellulose support (Song et al, 2018). The usage of bacterial cellulose as a template for aniline polymerization has also been undertaken.…”

Functionalization of Bacterial Cellulose Nonwoven by Poly(fluorophenol) to Improve Its Hydrophobicity and Durability

“…However, at higher monomer concentrations of up to 20 mM, the polymerization yield decreased when the incremental tendency of the contact angle began to weaken and reached the maximum value. This is explained by the CF 2 on the fiber surface being nearly saturated; therefore, increasing the deposition of fluorophenol concentration did not have a significant impact on the wettability of the fiber surface (Song et al, 2018).…”

“…As shown in Figure 1, BC was first swelled as described by Song et al (2018). After swelling, laccase was entrapped into swelled BC nanofiber by adding 20% (W/V) of laccase (245.5 U/ml) to 2 ± 0.8 g of BC in a 10 ml acetate buffer (pH 5, 0.1 M) for 30 min.…”

Conductive bacterial cellulose by in situ laccase polymerization of aniline

“…The polymers like polyaniline, synthesized by laccase, have been described as potential dyestuff for materials green coloration. The products of enzymatic polymerization of some substrates undergo coloration and are able to confer color to the substrates when applied in situ [21, 22], as can be found in literature [16, 23, 24]. Previously Song et al .…”

“…Previously Song et al . explored the biocoloration of BC by laccase-assisted polymerization of phenolics and found that this substrate is easily colored by the new polymers formed revealing leaching resistance when washed [24]. Another study was conducted by Jing et al describing the production of colored and conductive cotton by laccase-assisted polymerization aniline [25].…”