Bio-Based Solvents for Green Extraction of Lipids from Oleaginous Yeast Biomass for Sustainable Aviation Biofuel

Abstract

Abstract:Lipid-based oleaginous microorganisms are potential candidates and resources for the sustainable production of biofuels. This study was designed to evaluate the performance of several alternative bio-based solvents for extracting lipids from yeasts. We used experimental design and simulation with Hansen solubility simulations and the conductor-like screening model for realistic solvation (COSMO-RS) to simulate the solubilization of lipids in each of these solvents. Lipid extracts were analyzed by high performance thin-layer chromatography (HPTLC) to obtain the distribution of lipids classes and gas chromatography coupled with a flame ionization detector (GC/FID) to obtain fatty acid profiles. Our aim was to correlate simulation with experimentation for extraction and solvation of lipids with bio-based solvents in order to make a preliminary evaluation for the replacement of hexane to extract lipids from microorganisms. Differences between theory and practice were noted for several solvents, such as CPME, MeTHF and ethyl acetate, which appeared to be good candidates to replace hexane.

Green extraction of oil from Carum carvi seeds using bio‐based solvent and supercritical fluid: Evaluation of its antioxidant and anti‐inflammatory activities

“…Ben Youssef et al ., extracted date palm seeds of three cultivars (Deglet Nour, Allig, and Belah) with the two solvents (hexane and MeTHF) for 8 h by soxhlet and reported a cultivar‐dependent effect where MeTHF was the best solvent to extract oil from Bellah and Allig varieties while hexane remained the better solvent to extract oil from Deglet Nour variety. Moreover, MeTHF appeared to be a good candidate to replace hexane for lipid extraction from oleaginous yeast biomass …”

“Bligh and Dyer” and Folch Methods for Solid–Liquid–Liquid Extraction of Lipids from Microorganisms. Comprehension of Solvatation Mechanisms and towards Substitution with Alternative Solvents

“…The protocol of quantification of phospholipids and neutral lipids was explained in our previous work: Breil et al [11]. …”

“…However, these methods have serious disadvantages in terms of safety, especially with chloroform, which is highly toxic and carcinogenic [11], making it inappropriate for large-scale application.…”

Cyclopentyl Methyl Ether (CPME): A Versatile Eco‐Friendly Solvent for Applications in Biotechnology and Biorefineries

“…Extractants are commonly dissolved in water-immiscible solvents to simultaneously control the concentration and reduce viscosity while diluents should display high solubility in the extractant and conversely low solubility in water. Breil et al [56] compared the behavior of CPME with other solvents in extracting lipids from oleaginousy east. Nevertheless, archetypal ethers (Et 2 O, THF,1 ,4-dioxane), because of their low boiling points and relatively high watersolubility,a re often not the desired options.…”

“…[3a] CPME is particularly attractive fort he extractiono fd ifferent lipids from biomass, [55] which constitutes ap romisingo ption for the sustainable production of liquid biofuels. Breil et al [56] compared the behavior of CPME with other solvents in extracting lipids from oleaginousy east. An experimental study,b ased on the oil extraction from Yarrowia lipolytica IFP29 by using gas chromatography and high-performance thin-layer chromatography (HPTLC),w as compared with two theoretical approaches.…”