Bio-Based Self-Healing Concrete: From Research to Field Application

Tziviloglou, Eirini; Tittelboom, Kim Van; Palin, Damian; Wang, Jianyun; Sierra-Beltran, M.G.; Erşan, Yusuf Çağatay; Mors, R.M.; Wiktor, Virginie; Jonkers, H.M.; Schlangen, Erik; Belie, Nele De

doi:10.1007/12_2015_332

Cited by 52 publications

(16 citation statements)

References 44 publications

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“…It is believed that the MICP process is driven primarly by urea hydrolysis [1, 10, 14, 17, 25, 35]. Caclium carbonate precipitation and the crystal morphology are likely influenced by two major factors: i) biochemical induction and/or ii) purely chemical induction.…”

Section: Resultsmentioning

confidence: 99%

“…It is being investigated for possible usefulness in a multitude of applications including underground storage of carbon, healing masonry structures of archaeological importance, and long-term sealing of geologic cracks in large-scale structures [3, 15, 20–24]. The MICP phenomenon has been exhaustively studied in bulk systems, sand columns [20, 25, 26], and bio-cementation processes [26, 27] to understand the strength provided by the CaCO 3 . The bacterial involvement has also been explored to understand its importance, control mineral deposition rate, urease activity, crystal size and participation of bacterial cells.…”

Section: Introductionmentioning

confidence: 99%

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Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface

et al. 2019

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The bacterium Sporosarcina pasteurii (SP) is known for its ability to cause the phenomenon of microbially induced calcium carbonate precipitation (MICP). We explored bacterial participation in the initial stages of the MICP process at the cellular length scale under two different growth environments (a) liquid culture (b) MICP in a soft agar (0.5%) column. In the liquid culture, ex-situ imaging of the cellular environment indicated that S. pasteurii was facilitating nucleation of nanoscale crystals of calcium carbonate on bacterial cell surface and its growth via ureolysis. During the same period, the meso-scale environment (bulk medium) was found to have overgrown calcium carbonate crystals. The effect of media components (urea, CaCl2), presence of live and dead in the growth medium were explored. The agar column method allows for in-situ visualization of the phenomena, and using this platform, we found conclusive evidence of the bacterial cell surface facilitating formation of nanoscale crystals in the microenvironment. Here also the bulk environment or the meso-scale environment was found to possess overgrown calcium carbonate crystals. Extensive elemental analysis using Energy dispersive X-ray spectroscopy (EDS) and X-ray powder diffraction (XRD), confirmed that the crystals to be calcium carbonate, and two different polymorphs (calcite and vaterite) were identified. Active participation of S. pasteurii cell surface as the site of calcium carbonate precipitation has been shown using EDS elemental mapping with Scanning transmission electron microscopy (STEM) and scanning electron microscopy (SEM).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface

et al. 2019

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“…They were cracked in a four-point bending setup and a comparison of the self-healing efficiency was done. The same size of beams and a similar test approach was used to investigate the crack filling in concrete with ureolytic mixed self-protected bacterial cultures [9]. Araújo et al [10] cast beams (40 cm × 20 cm × 250 cm) with concrete containing mixed-in glass capsules or polymethyl methacrylate capsules (both filled with water repellent agent) to validate the self-sealing efficiency of these two encapsulation methods.…”

Section: Introductionmentioning

confidence: 99%

“…Al-Tabbaa et al [24] reported that the panel with the microcapsules was able to heal cracks faster and more complete than a control panel, resulting in a significant impermeability recovery. Aside from the application of self-healing additives in self-healing concrete, several case studies with self-healing repair products have also been reported [9,20,21,25].…”

Section: Introductionmentioning

confidence: 99%

First Large Scale Application with Self-Healing Concrete in Belgium: Analysis of the Laboratory Control Tests

et al. 2020

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Due to the negative impact of construction processes on the environment and a decrease in investments, there is a need for concrete structures to operate longer while maintaining their high performance. Self-healing concrete has the ability to heal itself when it is cracked, thereby protecting the interior matrix as well as the reinforcement steel, resulting in an increased service life. Most research has focused on mortar specimens at lab-scale. Yet, to demonstrate the feasibility of applying self-healing concrete in practice, demonstrators of large-scale applications are necessary. A roof slab of an inspection pit was cast with bacterial self-healing concrete and is now in normal operation. As a bacterial additive to the concrete, a mixture called MUC+, made out of a Mixed Ureolytic Culture together with anaerobic granular bacteria, was added to the concrete during mixing. This article reports on the tests carried out on laboratory control specimens made from the same concrete batch, as well as the findings of an inspection of the roof slab under operating conditions. Lab tests showed that cracks at the bottom of specimens and subjected to wet/dry cycles had the best visual crack closure. Additionally, the sealing efficiency of cracked specimens submersed for 27 weeks in water, measured by means of a water permeability setup, was at least equal to 90%, with an efficiency of at least 98.5% for the largest part of the specimens. An inspection of the roof slab showed no signs of cracking, yet favorable conditions for healing were observed. So, despite the high healing potential that was recorded during lab experiments, an assessment under real-life conditions was not yet possible.

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“…So far, regardless of the proposed metabolic pathway, MSCC perform better than conventional mortars in terms of water tightness regain 7,9,10 . Such regain in functionality is desired to hinder the ingress of aforementioned aggressive substances and to protect the good state of the rebar 11–13 . In previous microbe-based self-healing studies, it was claimed that biomineralization in microcracks is an effective method for protection of the steel reinforcement 4–6,9,14 .…”

Section: Introductionmentioning

confidence: 99%

Nitrite producing bacteria inhibit reinforcement bar corrosion in cementitious materials

Erşan

Tittelboom

Boon

et al. 2018

Sci Rep

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Chemicals and synthetic coatings are widely used to protect steel against corrosion. Bio-based corrosion inhibition strategies can be an alternative in the arising bioeconomy era. To maintain the good state of steel reinforcement in cracked concrete, microbe-based self-healing cementitious composites (MSCC) have been developed. Yet, proposed strategies involve reasonably slow crack filling by biomineralization and thus risk the possible rebar corrosion during crack healing. Here we upgrade the rebar protection to a higher level by combining MSCC with microbial induced corrosion inhibition. Presented NO reducing bacterial granules inhibit rebar corrosion by producing the anodic corrosion inhibitor NO and meanwhile heal a 300-µm-wide crack in 28 days. During 120 days exposure to 0.5 M Cl solution, the rebars in cracked MSCC keep showing open circuit potentials above the critical value of -250 mV and they lose less than 2% of the total rebar material which corresponds to half the material loss in cracked plain mortar. Overall, the obtained rebar protection performance is comparable with that of uncracked mortar and mortar containing chemical inhibitor, hence the microbe-based system becomes an alternative to the traditional methods.

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Bio-Based Self-Healing Concrete: From Research to Field Application

Cited by 52 publications

References 44 publications

Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface

Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface

First Large Scale Application with Self-Healing Concrete in Belgium: Analysis of the Laboratory Control Tests

Nitrite producing bacteria inhibit reinforcement bar corrosion in cementitious materials

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