Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of Aquilaria sinensis

Feng, Jie; Yang, Xiu‐Wei; Wang, Rufeng

doi:10.1016/j.phytochem.2010.11.025

Cited by 151 publications

(118 citation statements)

References 25 publications

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“…Gilg has been reported to inhibit α-glucosidase. 28 Here we report the α-glucosidase inhibitory activity of three species of Swertia and the xanthones 1 and 6 in addition to 4. Acarbose, the commercial α-glucosidase inhibitor had much higher activity than the isolated compounds and the crude extracts.…”

Section: Glycosidase Inhibitory Activitymentioning

confidence: 87%

Antioxidant, anti-acetylcholinesterase and anti-glycosidase properties of three species of Swertia, their xanthones and amarogentin: A comparative study

Nag

Das²,

Das³

et al. 2015

Phcog J

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Aim:The aim of the study was to analyze the antioxidant, anti-amylase, anti-glucosidase and antiacetylcholinesterase (anti-AChE) properties of the leafy shoots of three Indian species of Swertia e.g. Swertia chirata and its substitutes Swertia bimaculata, and Swertia decussata, their xanthones and amarogentin. Methods: Antioxidant activity of the methanolic extracts of leafy shoots was measured in terms of DPPH, superoxide and nitric oxide radical scavenging activities as well as metal chelating properties. Enzyme inhibitory properties were measured using AChE, α-amylase and α-glucosidase respectively. Five xanthones bellidifolin (1), swerchirin (2), decussatin (3), mangiferin (4) and 1-hydroxy-3,5,8-trimethoxy xanthone (6) and one iridoid, amarogentin (5) were isolated from Swertia chirata. The activities of the isolated components were compared. Results: Swertia chirata exhibited best antioxidant and antiAChE properties than the other two species. The plants also possessed α-glucosidase inhibitory properties but weak α-amylase inhibitory activity. Highest activities were observed in Swertia chirata. We report here, for the first time, the antioxidant, anti-AChE and anti-glycosidase activity of 1-hydroxy-3,5,8-trimethoxy xanthone. This xanthone had strongest DPPH radical scavenging activity and anti-AChE property. Conclusion: The results suggest the beneficial effects of the xanthones of Swertia chirata. But further study should be carried out to prove the efficacy in vivo.

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Section: Glycosidase Inhibitory Activitymentioning

confidence: 87%

Antioxidant, anti-acetylcholinesterase and anti-glycosidase properties of three species of Swertia, their xanthones and amarogentin: A comparative study

Nag

Das²,

Das³

et al. 2015

Phcog J

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“…Its molecular formula was assigned to be H COSY and HMBC spectra, which also were similar to those of 1, compound 2 was determined to be 3α-hydroxy-chirat-16-en-24-oic acid and named kouitchenoid B. Compounds 1-4 were evaluated fort their α-glucosiade inhibitory activity using p-nitrophenyl-α-Dglucopyranoside (PNPG) as the substrate [8]. Although, not stronger than the activity of the reference drug acarbose, these compounds still exerted mild inhibitory activity against α-glucosidase (Table 2).…”

Section: Resultsmentioning

confidence: 98%

“…α-Glucosidase (from Saccharomyces cerevisiae, Sigma-Aldrich, St. Louis, MO, USA) inhibitory activities were determined by using p-nitrophenyl-α-D-glucopyranoside (PNPG) as the substrate, according to the reported method [8]. Briefly, 20 μL of enzyme solution [0.6 U/mL α-glucosidase in 0.1 M potassium phosphate buffer (pH 6.8)] and 120 μL of the test compound in water containing 0.5% DMSO were mixed, and was preincubated for 15 min at 37 °C prior to initiation of the reaction by adding the substrate.…”

Section: In Vitro Inhibitory Activity Against α-Glucosidasementioning

confidence: 99%

Two New Chiratane-Type Triterpenoids from Swertia kouitchensis

Wan

Liu

Lin³

et al. 2013

Molecules

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Abstract:Two rare new chiratane-type triterpenoids, kouitchenoids A and B (1, 2), together with oleanolic acid (3) and ursolic acid (4), were isolated from ethanol extract of Swertia kouitchensis. The new structures were determined by the analysis of MS and NMR data. In addition, compounds 1-4 showed moderate inhibitory activity against the α-glucosidase (with IC 50 values ranging from 1,812 to 2,027 μM).

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“…The assay was conducted for each extracts (300 µL) in 96-well plates (SPL Lifescience Co., Pocheon, Korea) with a microplate reader (UVM340, Biochrom, Cambridge, UK) according to procedures previously described (10). Briefly, the enzyme (α-glucosidase) and substrate (p-NPG) were dissolved in 67 mM sodium phosphate buffer (pH 6.8) at 0.5 U/mL and 5 mM, respectively.…”

Section: α-Glucosidase Inhibition Assaymentioning

confidence: 99%

ACE and α-glucosidase Inhibitory Activity of the Glucosinolates in Dolsan Leaf Mustard Pickle during Storage

Son¹,

Oh²,

Tsukamoto³

et al. 2016

KSBB Journal

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This study was carried out for investigate that physiological activity, quantification and qualitative were sinigrin of Dolsan leaf mustard pickle (DLMP) during storage. sinigrin contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. ACE inhibitory activity was ranged between 43.2 and 79.4%. DLMP methanol extracts demonstrated highest ACE inhibitory activity at 79.4% on day 14, whereas DLMP ethanol extracts demonstrated highest Angiotensin I-converting Enzyme (ACE) inhibitory activity of 43.2% at day 0. The α-glucosidase inhibitory activity of positive control 0.02% (v/v) acarbose was 78%. The DLMP methanol extracts had the highest α-glucosidase inhibitory activity at 64.0% on day 14, whereas DLMP ethanol extracts had the lowest α-glucosidase inhibitory activity of 42.8% at day 28. Sinigrin was high in DLMP methanol extracts at 49.55 µg/ml on day 14 of storage. Sinigrin standard was eluted at 2.73 min and MS analysis was m/z 283.03 along with fragment ions at m/z 204 and 149.06. These data show that sinigrin formed desulfo-gluco-Sinigrin concentration increased until day 14 and then decreased after that. DLMP methanol extracts had consistently higher sinigrin concentration than DLMP acetonitrile extracts during 28 days of storage.

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Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of Aquilaria sinensis

Cited by 151 publications

References 25 publications

Antioxidant, anti-acetylcholinesterase and anti-glycosidase properties of three species of Swertia, their xanthones and amarogentin: A comparative study

Antioxidant, anti-acetylcholinesterase and anti-glycosidase properties of three species of Swertia, their xanthones and amarogentin: A comparative study

Two New Chiratane-Type Triterpenoids from Swertia kouitchensis

ACE and α-glucosidase Inhibitory Activity of the Glucosinolates in Dolsan Leaf Mustard Pickle during Storage

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