Bio‐aerosol fluorescence sensor

Reyes, Felix; Jeys, T. H.; Newbury, Nathan R.; Primmerman, C. A.; Rowe, G. S.; Sánchez, A.

doi:10.1002/(sici)1520-6521(1999)3:4/5<240::aid-fact3>3.0.co;2-#

Cited by 74 publications

(43 citation statements)

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“…However, these technical and scientific developments have been significant contributions to the scientific community (e.g. Hill et al, 1995;Cheng et al, 1999;Reyes et al, 1999;Seaver et al, 1999;Kopczynski et al, 2005;Cabredo et al, 2007;Campbell et al, 2007;Manninen et al, 2008;Pan et al, 2009;Sivaprakasam et al, 2009).…”

Section: Modern Analysis Methodsmentioning

confidence: 99%

“…Fluorescence microscopy is both tedious and labour intensive, although the use of fluorescence aids the identification of biological particles compared to light microscopy. Recently, more automatic techniques have been attempted, including computer analysis of microscopic images (Carrera et al, 2005) and fluorescence spectroscopy (Reyes et al, 1999;Courvoisier et al, 2008). Other modern fluorescence-based techniques will be described in the next section.…”

Section: Light Microscopymentioning

confidence: 99%

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Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,159

1,114

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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Section: Modern Analysis Methodsmentioning

confidence: 99%

Section: Light Microscopymentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,159

1,114

View full text Add to dashboard Cite

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“…They are also relevant to other types of instruments. Several bioaerosol detectors measure UV fluorescence from biological particles [14][15][16][17][18][19] . More intense UV excitation generally leads to more intense fluorescence 20 , which is beneficial, but if the excitation is too intense the aerosol particles will be damaged potentially interfering with more selective methods of subsequent analysis.…”

Section: Introductionmentioning

confidence: 99%

Desorption/Ionization Fluence Thresholds and Improved Mass Spectral Consistency Measured Using a Flattop Laser Profile in the Bioaerosol Mass Spectrometry of Single Bacillus Endospores

et al. 2005

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Bioaerosol mass spectrometry (BAMS) is being developed to analyze and identify biological aerosols in real-time. Mass spectra of individual Bacillus endospores were measured here with a bipolar aerosol time-of-flight mass spectrometer in which molecular desorption and ionization were produced using a single laser pulse from a Q-switched, frequency-quadrupled Nd:YAG laser that was modified to have an approximately flattop profile. The flattened laser profile allowed the minimum fluence required to desorb and ionize significant numbers of ions from single aerosol particles to be determined. For Bacillus spores this threshold had a mean value of approximately 1 nJ/µm 2 (0.1 J/cm 2 ). Thresholds for individual spores, however, could apparently deviate by 20% or more from the mean. Threshold distributions for clumps of MS2 bacteriophage and bovine serum albumin were subsequently determined. Finally, the flattened profile was observed to increase the reproducibility of single spore mass spectra. This is consistent with the general conclusions of our earlier paper on the fluence * E-mail: frank1@llnl.gov Telephone: 925-423-5068 Fax: 925-424-2778 2 dependence of single spore mass spectra and is particularly significant because it is expected to enable more robust differentiation and identification of single bioaerosol particles.

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“…On the other hand, optical techniques provide information in ''real time'' but until now lack in specificity. In particular, several optical systems, based on fluorescence [11][12][13][14] and/or elastic scattering 15,16 have been developed to distinguish bio-from non-bioaerosols. The most advanced experiments address individual aerosol particles, within which fluorescence is spectrally analyzed.…”

Section: Introductionmentioning

confidence: 99%

Identification of biological microparticles using ultrafast depletion spectroscopy

Courvoisier¹,

Bonacina²,

Boutou³

et al. 2008

Faraday Discuss.

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We show how an ultrafast pump-pump excitation induces strong fluorescence depletion in biological samples, such as bacteria-containing droplets, in contrast with fluorescent interferents, such as polycyclic aromatic compounds, despite similar spectroscopic properties. Application to the optical remote discrimination of biotic versus non-biotic particles is proposed. Further improvement is required to allow the discrimination of one pathogenic among other non-pathogenic micro-organisms. This improved selectivity may be reached with optimal coherent control experiments, as discussed in the paper.

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Bio‐aerosol fluorescence sensor

Cited by 74 publications

References 1 publication

Primary biological aerosol particles in the atmosphere: a review

Primary biological aerosol particles in the atmosphere: a review

Desorption/Ionization Fluence Thresholds and Improved Mass Spectral Consistency Measured Using a Flattop Laser Profile in the Bioaerosol Mass Spectrometry of Single Bacillus Endospores

Identification of biological microparticles using ultrafast depletion spectroscopy

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