Binding to DNA of selected lexitropsins and effects on prokaryotic topoisomerase activity

Burckhardt, G.; Luck, G.; Störl, K.; Zimmer, C.; Lown, J. William

doi:10.1016/0167-4781(93)90123-u

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

1993

DOI: 10.1016/0167-4781(93)90123-u

|View full text |Cite

Binding to DNA of selected lexitropsins and effects on prokaryotic topoisomerase activity

G. Burckhardt

G. Luck

K. Störl

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1997

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

DNA binding properties of minor groove binders and their influence on the topoisomerase II cleavage reaction

Bell¹,

Kittler²,

Löber³

et al. 1997

J. Mol. Recognit.

View full text Add to dashboard Cite

We present titrations of the human delta beta-globin gene region with DNA minor groove binders netropsin, bisnetropsin, distamycin, chromomycin and four bis-quaternary ammonium compounds in the presence of calf thymus topoisomerase II and DNase I. With increasing ligand concentration, stimulation and inhibition of enzyme activity were detected and quantitatively evaluated. Additionally we show a second type of stimulation, the appearance of strong new topoisomerase II cleavage sites at high ligand concentrations. The specific binding sites of the minor groove binders of the DNA sequence and their microscopic binding constants were determined from DNase I footprints. A binding mechanism for minor groove binders is proposed in order to explain these results especially when ligand concentration is increased.

show abstract

DNA binding properties of minor groove binders and their influence on the topoisomerase II cleavage reaction

Bell¹,

Kittler²,

Löber³

et al. 1997

J. Mol. Recognit.

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Binding to DNA of selected lexitropsins and effects on prokaryotic topoisomerase activity

Cited by 1 publication

References 26 publications

DNA binding properties of minor groove binders and their influence on the topoisomerase II cleavage reaction

DNA binding properties of minor groove binders and their influence on the topoisomerase II cleavage reaction

Contact Info

Product

Resources

About