Binding the Atypical RA Domain of Ste50p to the Unfolded Opy2p Cytoplasmic Tail Is Essential for the High-Osmolarity Glycerol Pathway

Ekiel, Irena; Sulea, Traian; Jansen, Gregor; Kowalik, M.; Minailiuc, Ovidiu M.; Cheng, Jing; Harcus, Doreen; Cygler, Mirosław; Whiteway, Malcolm; Wu, Cunle

doi:10.1091/mbc.e09-07-0645

Cited by 34 publications

(52 citation statements)

References 45 publications

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“…Thus, Mig1 and Mig2 may specifically regulate the filamentation MAPK. The inhibitory effect of Opy2/Mig1 might occur through Ste50, which is a target of Kss1 (Jansen et al 2001;Ramezani-Rad 2003;Ekiel et al 2009;Yamamoto et al 2010). Indeed, overexpression of Ste50 also dampened FUS1-HIS3 expression ( Figure 6B).…”

Section: Mig1 and Opy2 Contribute To A Pathwayspecific Responsementioning

confidence: 97%

“…Opy2 functions in the HOG pathway (Wu et al 2006;Ekiel et al 2009;Yang et al 2009;Yamamoto et al 2010;Cappell and Dohlman 2011) and may also regulate the filamentous growth pathway (Yang et al 2009;Yamamoto et al 2010), although this possibility has not been extensively explored (Wu et al 2006;Chen and Thorner 2007). To determine whether Opy2 regulates the filamentous growth pathway, the OPY2 gene was deleted in strains of the filamentous ( P 1278b) background (Liu et al 1996).…”

Section: Opy2 Regulates the Filamentous Growth Pathway By Plasma-membmentioning

confidence: 99%

“…Two alleles were identified: OPY2 G207D was partially defective for pathway activity ( Figure 2E, PKss1). OPY2 G207D contains a mutation in a Ste50-binding site ( Figure 3A) (Ekiel et al 2009;Yamamoto et al 2010) and was partially defective for binding to Ste50 by two-hybrid analysis ( Figure 2F).…”

Section: Opy2 Regulates the Filamentous Growth Pathway By Plasma-membmentioning

confidence: 99%

“…This connection was uncovered by characterizing the newly identified plasma-membrane regulator Opy2 (Wu et al 2006;Ekiel et al 2009;Yang et al 2009;Yamamoto et al 2010;Cappell and Dohlman 2011), which regulates the Ste11 branch of the high-osmolarity glycerol response (HOG) MAPK pathway (Hohmann et al 2007;Saito 2010). Opy2 is also thought to regulate the filamentous growth pathway (Yang et al 2009;Yamamoto et al 2010).…”

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confidence: 99%

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The Filamentous Growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 Transcriptional Repressors in Saccharomyces cerevisiae

Karunanithi

Cullen

2012

Genetics

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In the budding yeast S. cerevisiae, nutrient limitation induces a MAPK pathway that regulates filamentous growth and biofilm/mat formation. How nutrient levels feed into the regulation of the filamentous growth pathway is not entirely clear. We characterized a newly identified MAPK regulatory protein of the filamentous growth pathway, Opy2. A two-hybrid screen with the cytosolic domain of Opy2 uncovered new interacting partners including a transcriptional repressor that functions in the AMPK pathway, Mig1, and its close functional homolog, Mig2. Mig1 and Mig2 coregulated the filamentous growth pathway in response to glucose limitation, as did the AMP kinase Snf1. In addition to associating with Opy2, Mig1 and Mig2 interacted with other regulators of the filamentous growth pathway including the cytosolic domain of the signaling mucin Msb2, the MAP kinase kinase Ste7, and the MAP kinase Kss1. As for Opy2, Mig1 overproduction dampened the pheromone response pathway, which implicates Mig1 and Opy2 as potential regulators of pathway specificity. Taken together, our findings provide the first regulatory link in yeast between components of the AMPK pathway and a MAPK pathway that controls cellular differentiation.

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Section: Mig1 and Opy2 Contribute To A Pathwayspecific Responsementioning

confidence: 97%

Section: Opy2 Regulates the Filamentous Growth Pathway By Plasma-membmentioning

confidence: 99%

Section: Opy2 Regulates the Filamentous Growth Pathway By Plasma-membmentioning

confidence: 99%

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confidence: 99%

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The Filamentous Growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 Transcriptional Repressors in Saccharomyces cerevisiae

Karunanithi

Cullen

2012

Genetics

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“…We have recently shown that the HMH domains bind to the extracellular Cys-rich region of Opy2 (22,29). Opy2 is a single-path transmembrane protein whose cytoplasmic tail binds to the Ste50 adaptor protein (36,37). Because Ste50 also binds to Ste11 (27,28), Opy2 indirectly recruits Ste11 to the plasma membrane.…”

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confidence: 99%

Scaffold Protein Ahk1, Which Associates with Hkr1, Sho1, Ste11, and Pbs2, Inhibits Cross Talk Signaling from the Hkr1 Osmosensor to the Kss1 Mitogen-Activated Protein Kinase

Nishimura

Yamamoto

Oyama

et al. 2016

Molecular and Cellular Biology

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Negative feedback-loop mechanisms regulating HOG- and pheromone-MAPK signaling in yeast

et al. 2020

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Binding the Atypical RA Domain of Ste50p to the Unfolded Opy2p Cytoplasmic Tail Is Essential for the High-Osmolarity Glycerol Pathway

Abstract: Activation of the high-osmolarity glycerol (HOG) pathway for osmoregulation in the yeast

Cited by 34 publications

References 45 publications

The Filamentous Growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 Transcriptional Repressors in Saccharomyces cerevisiae

The Filamentous Growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 Transcriptional Repressors in Saccharomyces cerevisiae

Scaffold Protein Ahk1, Which Associates with Hkr1, Sho1, Ste11, and Pbs2, Inhibits Cross Talk Signaling from the Hkr1 Osmosensor to the Kss1 Mitogen-Activated Protein Kinase

Negative feedback-loop mechanisms regulating HOG- and pheromone-MAPK signaling in yeast

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