Binding Studies of Small Molecules to Telomeric Quadruplex DNA for Targeted Singlet Oxygen Production

Kopkalli, Yasemin; Biegel, Craig; Khemraj, Ryan; Davenport, Lesley

doi:10.1016/j.bpj.2014.11.2166

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2019

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“…Unlike TMPyP4 and related planar porphyrins, which have demonstrated DNA photocleavage activity, no studies investigating the DNA‐photocleavage activity of NMM have been reported. One published abstract has interestingly reported that NMM photosensitized the generation of singlet oxygen in the presence of G4 but not duplex DNA . We have therefore investigated the G4 photocleavage potential of NMM, and report here that this nonplanar porphyrin is an extremely selective photocleavage agent for G4 versus duplex DNA.…”

Section: Methodsmentioning

confidence: 90%

N‐Methylmesoporphyrin IX Exhibits G‐Quadruplex‐Specific Photocleavage Activity

et al. 2019

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N‐Methylmesoporphyrin IX (NMM) has long been known as a G‐quadruplex DNA (G4) ligand. However, there has been little investigation into its G‐quadruplex photocleavage activity. Herein, we demonstrate that NMM is a highly selective photocleavage agent for G4 structures but not duplex DNA. Analysis of the cleavage products by PAGE demonstrates that G4 photocleavage by NMM occurs at sites similar to those cleaved by TMPyP4, a nonselective DNA photocleavage agent. Although NMM is shown here to generate singlet oxygen in the presence of both duplex and G4, the lack of increased photocleavage in D2O indicated that singlet oxygen is not involved in the photocleavage of G4 by NMM.

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Section: Methodsmentioning

confidence: 90%