Binding Properties and Immunolocalization of a Fatty Acid–binding Protein in Giardia Lamblia

Hassan, Sabih-Ul; Maache, Mimoun; Guardia, R. Díaz de la; Córdova, Ofelia; García, Javier Fernández; Galiana, M.; Castroviejo, Darío Acuña; Martins, Márcio O.; Osuna, Antonio

doi:10.1645/ge-3352

Cited by 5 publications

(11 citation statements)

References 55 publications

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“…Giardial lipid and fatty-acid transport proteins may also participate in translocating lipid molecules that may travel along the microtubules to reach perinuclear membranes. The presence of a fatty-acid binding protein (~8 KD) has been reported in Giardia (Hassan et al 2005). The transport of ER/perinuclear PG via exocytic vesicles may be regulated by microtubule filaments and not by actin cytoskeleton (Fig.…”

Section: Import Of Lipids and Fatty Acids By Giardiamentioning

confidence: 97%

Lipid metabolism inGiardia: a post-genomic perspective

et al. 2010

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SUMMARY Giardia lamblia, a protozoan parasite, infects a wide variety of vertebrates, including humans. Studies indicate that this anaerobic protist possesses a limited ability to synthesize lipid molecules de novo and depends on supplies from its environment for growth and differentiation. It has been suggested that most lipids and fatty acids are taken up by endocytic and non-endocytic pathways and are used by Giardia for energy production and membrane/organelle biosynthesis. The purpose of this article is to provide an update on recent progress in the field of lipid research of this parasite and the validation of lipid metabolic pathways through recent genomic information. Based on current cellular, biochemical and genomic data, a comprehensive pathway has been proposed to facilitate our understanding of lipid and fatty acid metabolism/syntheses in this waterborne pathogen. We envision that the current review will be helpful in identifying targets from the pathways that could be used to design novel therapies to control giardiasis and related diseases.

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Section: Import Of Lipids and Fatty Acids By Giardiamentioning

confidence: 97%

Lipid metabolism inGiardia: a post-genomic perspective

et al. 2010

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“…After purification (Table I), the final yield was 62.71%, i.e., a yield increase when compared with the purification procedures using an affinity column prepared with stearic acid as the ligand (Hasan et al, 2005). The method used here is, therefore, more efficient than the one previously described for the purification of these proteins in G. lamblia.…”

Section: Discussionmentioning

confidence: 82%

“…After the purification, the final yield was 62.71%; when stearic acid was used as the ligand, the yield was 53.13% (Hasan et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…The quantification of proteins was performed using the Bradford (1976) method, with bovine serum albumin as the protein standard; the purification yield of fatty acid binding proteins was determined using 11-(dansylamino) undecanoic acid (DAUDA) (Hasan et al, 2005) and a Hitachi F 2000 spectrofluorimeter.…”

Section: Determination Of the Purificationmentioning

confidence: 99%

“…It also possesses a high degree of immunogenicity (Hasan, Maache, et al, 2002). The variability in amino acid composition of HLBP and FABP proteins (Veerkamp et al, 1991;Arend et al, 2004) and their location on the parasite make it a good candidate for use as a vaccine.…”

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confidence: 99%

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Purification and Characterization of a Protein Capable of Binding To Fatty Acids and Bile Salts in Giardia lamblia

Guardia

López

Burgos

et al. 2011

Journal of Parasitology

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A specific fatty acid binding protein was isolated from Giardia lamblia, using an affinity column with butyric acid acting as a ligand in place of stearic acid. This method has proved to be more efficient than the one previously described using stearic acid as ligand. The purified fraction showed 8 electrophoretic bands of proteins, with molecular weights ranging between 8 and 80 kDa. This pattern is a consequence of the aggregation of a protein with a molecular weight of 8,215 Da, corresponding to the lower molecular weight band, the only one capable of binding to fatty acids. The labeled oleic acid bound to these purified proteins was replaced by a 100-fold greater concentration of taurocholate, glycocholate, deoxycholate, palmitic acid, and arachidonic acid, having a greater displacement of the bile salts than the free fatty acids.

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Host humoral immune response to Leishmania lipid‐binding protein

Maache

Azzouz

Guardia

et al. 2005

Parasite Immunology

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SUMMARY We report on the use of Leishmania donovani lipid-binding proteins (LBPs) as antigens capable of being recognized by serum from immunocompetent patients from southern Spain suffering from visceral leishmaniasis and from Peruvian patients with localized cutaneous leishmaniasis caused by Leishmania braziliensis. The absorbance found by immunoenzymatic techniques gave significantly different results for the serum samples from patients with and without leishmaniasis. Specificity by ELISA testing was 93.2% and sensibility 100%. Dot blots from human patient serum samples or naturally infected dogs from Spain gave similarly significant results. All the human serum samples from individuals with visceral leishmaniasis and the Leishmania-positive canine samples recognized two bands, with molecular weights of 8 and 57 kDa. The serum from individuals with cutaneous leishmaniasis caused by L. braziliensis recognized an additional band of 16 kDa. We discuss the role of Leishmania FABP and compare the immunological reactions found with serum samples from other protozoan infections such as toxoplasma and Chagas as well as bacterial infections such as tuberculosis and syphilis.

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Binding Properties and Immunolocalization of a Fatty Acid–binding Protein in Giardia Lamblia

Cited by 5 publications

References 55 publications

Lipid metabolism inGiardia: a post-genomic perspective

Lipid metabolism inGiardia: a post-genomic perspective

Purification and Characterization of a Protein Capable of Binding To Fatty Acids and Bile Salts in Giardia lamblia

Host humoral immune response to Leishmania lipid‐binding protein

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