Binding of β2-glycoprotein I to platelets: Effect of adenylate cyclase activity

Schousboe, Inger

doi:10.1016/0049-3848(80)90421-1

Cited by 112 publications

(54 citation statements)

References 23 publications

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“…9 One of the bridging proteins, which directly mediates attractive interactions between charged-charged, chargedneutral, and neutral-neutral phospholipid membranes, is β2GPI. 79,80 It was found that β2GPI binds to structures with negatively charged phospholipid molecules, thrombocytes, 81 erythrocytes, 80 and serum lipoproteins; 82,83 moreover, it binds to phospholipid layers with electrostatic and also hydrophobic interactions. 79,80,84 β2GPI has a positive ζ-potential of 17.80 mV.…”

Section: Discussionmentioning

confidence: 99%

Binding of plasma proteins to titanium dioxide nanotubes with different diameters

Kulkarni

Flašker

Lokar

et al. 2015

IJN

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Titanium and titanium alloys are considered to be one of the most applicable materials in medical devices because of their suitable properties, most importantly high corrosion resistance and the specific combination of strength with biocompatibility. In order to improve the biocompatibility of titanium surfaces, the current report initially focuses on specifying the topography of titanium dioxide (TiO 2 ) nanotubes (NTs) by electrochemical anodization. The zeta potential (ζ-potential) of NTs showed a negative value and confirmed the agreement between the measured and theoretically predicted dependence of ζ-potential on salt concentration, whereby the absolute value of ζ-potential diminished with increasing salt concentrations. We investigated binding of various plasma proteins with different sizes and charges using the bicinchoninic acid assay and immunofluorescence microscopy. Results showed effective and comparatively higher protein binding to NTs with 100 nm diameters (compared to 50 or 15 nm). We also showed a dose-dependent effect of serum amyloid A protein binding to NTs. These results and theoretical calculations of total available surface area for binding of proteins indicate that the largest surface area (also considering the NT lengths) is available for 100 nm NTs, with decreasing surface area for 50 and 15 nm NTs. These current investigations will have an impact on increasing the binding ability of biomedical devices in the body leading to increased durability of biomedical devices.

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Section: Discussionmentioning

confidence: 99%

Binding of plasma proteins to titanium dioxide nanotubes with different diameters

Kulkarni

Flašker

Lokar

et al. 2015

IJN

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“…The concentration of apolipoprotein H in adult serum is 170 mg͞liter (13), and we found similar concentrations in the neonatal lamb and piglet as well as FCS. Infusion of apolipoprotein H should bind circulating bacterial phosphatidylglycerol or cardiolipin, but the rate of binding may not be fast enough to prevent unliganded phospholipid from reaching the pulmonary circulation (14). Binding could inhibit the hypertensive effect, although it might actually promote the hypertensive effect because apolipoprotein H binds to endothelial cells (15).…”

Section: Purification and Identification Of The Active Componentsmentioning

confidence: 99%

“…Binding could inhibit the hypertensive effect, although it might actually promote the hypertensive effect because apolipoprotein H binds to endothelial cells (15). We therefore tested the effect of cardiolipin, which was mixed with a equimolar concentration of purified apolipoprotein H and allowed to stand overnight before testing, assuring that the binding reaction had gone to completion (14). The peak hypertensive effect and increase in plasma thromboxane were equivalent to that of the cardiolipin infused without apolipoprotein H (as in Fig.…”

Section: Purification and Identification Of The Active Componentsmentioning

confidence: 99%

Group B streptococcal phospholipid causes pulmonary hypertension

Curtis

Kim

Wehr

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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Group B Streptococcus is the most common cause of bacterial infection in the newborn. Infection in many cases causes persistent pulmonary hypertension, which impairs gas exchange in the lung. We purified the bacterial components causing pulmonary hypertension and identified them as cardiolipin and phosphatidylglycerol. Synthetic cardiolipin or phosphatidylglycerol also induced pulmonary hypertension in lambs. The recognition that bacterial phospholipids may cause pulmonary hypertension in newborns with Group B streptococcal infection opens new avenues for therapeutic intervention.G roup B Streptococcus (GBS) is the most frequent cause of sepsis and meningitis in the human newborn (1). Despite prompt treatment with antibiotics, neonates with GBS infection are often quite ill and the fatality rate is 5% (2). Respiratory distress, a prominent sign in these babies, is caused by pulmonary hypertension induced by the GBS infection (3). The pulmonary hypertension reflects an increase in pulmonary vascular resistance, which impairs exchange of oxygen and carbon dioxide. Infusion of live or heat-killed GBS into sheep promptly induces pulmonary hypertension, with little or no effect on systemic pressure (4). This response has been reproduced by many investigators, who also established that the pulmonary hypertension is not a consequence of simple embolization because infusion of latex beads of the same size as GBS caused no change in pulmonary physiology (5). The hypertension is mediated by an increase in thromboxane A 2 , so that treatment with cyclooxygenase inhibitors such as indomethacin or with thromboxane synthesis inhibitors prevents or reverses the increase in pulmonary pressure (5, 6). However, the component(s) of GBS that activate thromboxane synthesis are unknown. Guided by bioassays performed in neonatal lambs (7), we undertook the purification and identification of these components, using standard biochemical techniques. Materials and Methods Purification and Analysis of Pulmonary Hypertensive Compounds fromGBS. Biochemical fractionation procedures were linked in series to develop an initial purification protocol, which was modified to give the final protocol described here. At each step, fractions were assayed for pulmonary arterial hypertensive activity in 3-to 12-day-old lambs of Ϸ5 kg, as described (7). We previously demonstrated that pulmonary arterial pressure measurements were a valid surrogate for measurement of pulmonary vascular resistance in this animal model (7). Fractions were infused peripherally into the femoral vein, not directly into the pulmonary artery. Fractions were considered active if they increased pulmonary arterial pressure by at least 50% in at least two lambs tested on different days.GBS type III, strain 878 was grown to mid-or late-log phase (7), washed with sterile normal saline, heat-killed by incubation in a 60°C water bath for 90 min, pelleted by centrifugation, and resuspended in saline to an OD of 2.0 at 650 nm. Heat-killed GBS (3-gm wet weight) were extracted with 40 ml of ...

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“…Different physiological activities have been attributed to apoH, such as a possible role in the coagulation pathway, exhibiting an inhibitory effect on ADP-mediated platelet aggregations (16,17) and on prothrombinase activity (18). Moreover, apoH may be involved in the metabolism of triglyceride-rich lipoproteins as well as in the regulation of haemostasis and in the clearance of apoptotic bodies, mediating the phagocytic uptake of phosphatidylserine containing liposomes by macrophages (19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

Ragusa

Costa²,

Cefalù³

et al. 2006

Int J Mol Med

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Abstract. In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.

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Binding of β2-glycoprotein I to platelets: Effect of adenylate cyclase activity

Cited by 112 publications

References 23 publications

Binding of plasma proteins to titanium dioxide nanotubes with different diameters

Binding of plasma proteins to titanium dioxide nanotubes with different diameters

Group B streptococcal phospholipid causes pulmonary hypertension

RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

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